| http://purl.uniprot.org/citations/21511697 | http://www.w3.org/1999/02/22-rdf-syntax-ns#type | http://purl.uniprot.org/core/Journal_Citation |
| http://purl.uniprot.org/citations/21511697 | http://www.w3.org/2000/01/rdf-schema#comment | "AS160, a novel Akt substrate of 160 kDa, contains a Rab GTPase-activating protein (GAP) domain. The present study examined the role of Akt and AS160 in aquaporin-2 (AQP2) trafficking. The main strategy was to examine the changes in AQP2 translocation in response to small interfering RNA (siRNA)-mediated AS160 knockdown in mouse cortical collecting duct cells (M-1 cells and mpkCCDc14 cells). Short-term dDAVP treatment in M-1 cells stimulated phosphorylation of Akt (S473) and AS160, which was also seen in mpkCCDc14 cells. Conversely, the phosphoinositide 3-kinase (PI3K) inhibitor LY 294002 diminished phosphorylation of Akt (S473) and AS160. Moreover, siRNA-mediated Akt1 knockdown was associated with unchanged total AS160 but decreased phospho-AS160 expression, indicating that phosphorylation of AS160 is dependent on PI3K/Akt pathways. siRNA-mediated AS160 knockdown significantly decreased total AS160 and phospho-AS160 expression. Immunocytochemistry revealed that AS160 knockdown in mpkCCDc14 cells was associated with increased AQP2 density in the plasma membrane [135 ± 3% of control mpkCCDc14 cells (n = 65), P < 0.05, n = 64] despite the absence of dDAVP stimulation. Moreover, cell surface biotinylation assays of mpkCCDc14 cells with AS160 knockdown exhibited significantly higher AQP2 expression [150 ± 15% of control mpkCCDc14 cells (n = 3), P < 0.05, n = 3]. Taken together, PI3K/Akt pathways mediate the dDAVP-induced AS160 phosphorylation, and AS160 knockdown is associated with higher AQP2 expression in the plasma membrane. Since AS160 contains a GAP domain leading to a decrease in the active GTP-bound form of AS160 target Rab proteins for vesicle trafficking, decreased expression of AS160 is likely to play a role in the translocation of AQP2 to the plasma membrane."xsd:string |
| http://purl.uniprot.org/citations/21511697 | http://purl.org/dc/terms/identifier | "doi:10.1152/ajprenal.00519.2010"xsd:string |
| http://purl.uniprot.org/citations/21511697 | http://purl.uniprot.org/core/author | "Choi H.J."xsd:string |
| http://purl.uniprot.org/citations/21511697 | http://purl.uniprot.org/core/author | "Kim S.Y."xsd:string |
| http://purl.uniprot.org/citations/21511697 | http://purl.uniprot.org/core/author | "Lee Y.J."xsd:string |
| http://purl.uniprot.org/citations/21511697 | http://purl.uniprot.org/core/author | "Lim J.S."xsd:string |
| http://purl.uniprot.org/citations/21511697 | http://purl.uniprot.org/core/author | "Park E.J."xsd:string |
| http://purl.uniprot.org/citations/21511697 | http://purl.uniprot.org/core/author | "Kim H.Y."xsd:string |
| http://purl.uniprot.org/citations/21511697 | http://purl.uniprot.org/core/author | "Jung H.J."xsd:string |
| http://purl.uniprot.org/citations/21511697 | http://purl.uniprot.org/core/author | "Kwon T.H."xsd:string |
| http://purl.uniprot.org/citations/21511697 | http://purl.uniprot.org/core/date | "2011"xsd:gYear |
| http://purl.uniprot.org/citations/21511697 | http://purl.uniprot.org/core/name | "Am J Physiol Renal Physiol"xsd:string |
| http://purl.uniprot.org/citations/21511697 | http://purl.uniprot.org/core/pages | "F151-61"xsd:string |
| http://purl.uniprot.org/citations/21511697 | http://purl.uniprot.org/core/title | "Emerging role of Akt substrate protein AS160 in the regulation of AQP2 translocation."xsd:string |
| http://purl.uniprot.org/citations/21511697 | http://purl.uniprot.org/core/volume | "301"xsd:string |
| http://purl.uniprot.org/citations/21511697 | http://www.w3.org/2004/02/skos/core#exactMatch | http://purl.uniprot.org/pubmed/21511697 |
| http://purl.uniprot.org/citations/21511697 | http://xmlns.com/foaf/0.1/primaryTopicOf | https://pubmed.ncbi.nlm.nih.gov/21511697 |
| http://purl.uniprot.org/uniprot/#_E9Q6Q8-mappedCitation-21511697 | http://www.w3.org/1999/02/22-rdf-syntax-ns#object | http://purl.uniprot.org/citations/21511697 |
| http://purl.uniprot.org/uniprot/#_E0CX53-mappedCitation-21511697 | http://www.w3.org/1999/02/22-rdf-syntax-ns#object | http://purl.uniprot.org/citations/21511697 |
| http://purl.uniprot.org/uniprot/#_F6S7E8-mappedCitation-21511697 | http://www.w3.org/1999/02/22-rdf-syntax-ns#object | http://purl.uniprot.org/citations/21511697 |
| http://purl.uniprot.org/uniprot/#_F6SUV7-mappedCitation-21511697 | http://www.w3.org/1999/02/22-rdf-syntax-ns#object | http://purl.uniprot.org/citations/21511697 |
| http://purl.uniprot.org/uniprot/#_H7BX82-mappedCitation-21511697 | http://www.w3.org/1999/02/22-rdf-syntax-ns#object | http://purl.uniprot.org/citations/21511697 |
| http://purl.uniprot.org/uniprot/#_Q05BK2-mappedCitation-21511697 | http://www.w3.org/1999/02/22-rdf-syntax-ns#object | http://purl.uniprot.org/citations/21511697 |
| http://purl.uniprot.org/uniprot/#_Q3UQD4-mappedCitation-21511697 | http://www.w3.org/1999/02/22-rdf-syntax-ns#object | http://purl.uniprot.org/citations/21511697 |