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http://purl.uniprot.org/citations/21513557http://www.w3.org/1999/02/22-rdf-syntax-ns#typehttp://purl.uniprot.org/core/Journal_Citation
http://purl.uniprot.org/citations/21513557http://www.w3.org/2000/01/rdf-schema#comment"

Background

Allogeneic hematopoietic stem cell transplantation (allo-HSCT) leads to a prolonged state of immunodeficiency and requires reconstitution of normal T-cell immunity. Signal joint T-cell receptor excision DNA circles (sjTRECs) are markers of developmental proximity to the thymus that have been used to evaluate thymic function related to T-cell immune reconstitution after HSCT. To assess the proliferative history in different T-cell receptor beta variable region (TRBV) subfamilies of T cells after HSCT, expansion of TRBV subfamily-naive T cells was determined by analysis of a series of TRBV-BD1 sjTRECs.

Methods

sjTRECs levels were detected by real-time quantitative polymerase chain reaction (PCR) in peripheral blood mononuclear cells (PBMCs) from 43 Chinese acute leukemia patients who underwent allo-HSCT. Twenty-three TRBV-BD1 sjTRECs were amplified by semi-nested PCR. Sixteen age-matched healthy volunteers served as normal controls.

Results

sjTRECs levels were low or undetectable in the first 6 weeks after allo-HSCT and increased after 8 weeks post HSCT; however, sjTRECs levels at week 20 post-HSCT were still less than normal controls. Frequencies of TRBV subfamily sjTRECs in PBMCs from recipients at week 8 post-HSCT (29.17 ± 20.97%) or at week 16 post-HSCT (38.33 ± 9.03%) were significantly lower than those in donors (47.92 ± 13.82%) or recipients at pre-HSCT (45.83 ± 14.03%). However, frequencies of TRBV subfamily sjTRECs in recipients at week 30 post-HSCT (42.71 ± 21.62%) were similar to those in donors and recipients at pre-HSCT. sjTRECs levels in donors had a positive linear correlation with sjTRECs levels in recipients within 8-12 weeks post-HSCT. Patients with acute graft-versus-host disease (GVHD) or chronic GVHD had profoundly reduced TRECs levels during the first year post-HSCT. Frequencies of BV22-BD1 sjTRECs and BV23-BD1 sjTRECs in patients with GVHD were significantly lower than those in recipients at pre-HSCT, and the frequencies of BV22-BD1 sjTRECs in patients with GVHD were significantly lower than those in donors.

Conclusions

Reconstitution of thymic output function resulted in a period of immunodeficiency, with low or undetectable TRECs after transplantation, although fludarabine-based dose-reduced conditioning regimens were used. GVHD could affect reconstitution of thymic output function and reduce sjTRECs levels and frequencies of TRBV-BD1 sjTRECs. Low frequency of BV22-BD1 and BV23-BD1 sjTRECs might be associated with GVHD."xsd:string
http://purl.uniprot.org/citations/21513557http://purl.org/dc/terms/identifier"doi:10.1186/1756-8722-4-19"xsd:string
http://purl.uniprot.org/citations/21513557http://purl.uniprot.org/core/author"Chen S."xsd:string
http://purl.uniprot.org/citations/21513557http://purl.uniprot.org/core/author"Liu Q."xsd:string
http://purl.uniprot.org/citations/21513557http://purl.uniprot.org/core/author"Li Y."xsd:string
http://purl.uniprot.org/citations/21513557http://purl.uniprot.org/core/author"Wu J."xsd:string
http://purl.uniprot.org/citations/21513557http://purl.uniprot.org/core/author"Yang L."xsd:string
http://purl.uniprot.org/citations/21513557http://purl.uniprot.org/core/author"Wu X."xsd:string
http://purl.uniprot.org/citations/21513557http://purl.uniprot.org/core/author"Zhu K."xsd:string
http://purl.uniprot.org/citations/21513557http://purl.uniprot.org/core/author"Du X."xsd:string
http://purl.uniprot.org/citations/21513557http://purl.uniprot.org/core/date"2011"xsd:gYear
http://purl.uniprot.org/citations/21513557http://purl.uniprot.org/core/name"J Hematol Oncol"xsd:string
http://purl.uniprot.org/citations/21513557http://purl.uniprot.org/core/pages"19"xsd:string
http://purl.uniprot.org/citations/21513557http://purl.uniprot.org/core/title"Frequency analysis of TRBV subfamily sjTRECs to characterize T-cell reconstitution in acute leukemia patients after allogeneic hematopoietic stem cell transplantation."xsd:string
http://purl.uniprot.org/citations/21513557http://purl.uniprot.org/core/volume"4"xsd:string
http://purl.uniprot.org/citations/21513557http://www.w3.org/2004/02/skos/core#exactMatchhttp://purl.uniprot.org/pubmed/21513557
http://purl.uniprot.org/citations/21513557http://xmlns.com/foaf/0.1/primaryTopicOfhttps://pubmed.ncbi.nlm.nih.gov/21513557
http://purl.uniprot.org/uniprot/#_P0DTU4-mappedCitation-21513557http://www.w3.org/1999/02/22-rdf-syntax-ns#objecthttp://purl.uniprot.org/citations/21513557
http://purl.uniprot.org/uniprot/#_P0DSE2-mappedCitation-21513557http://www.w3.org/1999/02/22-rdf-syntax-ns#objecthttp://purl.uniprot.org/citations/21513557
http://purl.uniprot.org/uniprot/P0DSE2http://purl.uniprot.org/core/mappedCitationhttp://purl.uniprot.org/citations/21513557
http://purl.uniprot.org/uniprot/P0DTU4http://purl.uniprot.org/core/mappedCitationhttp://purl.uniprot.org/citations/21513557