| http://purl.uniprot.org/citations/21518758 | http://www.w3.org/1999/02/22-rdf-syntax-ns#type | http://purl.uniprot.org/core/Journal_Citation |
| http://purl.uniprot.org/citations/21518758 | http://www.w3.org/2000/01/rdf-schema#comment | "A plethora of peptides are generated intracellularly, and most peptide-human leukocyte antigen (HLA)-I interactions are of a transient, unproductive nature. Without a quality control mechanism, the HLA-I system would be stressed by futile attempts to present peptides not sufficient for the stable peptide-HLA-I complex formation required for long term presentation. Tapasin is thought to be central to this essential quality control, but the underlying mechanisms remain unknown. Here, we report that the N-terminal region of tapasin, Tpn(1-87), assisted folding of peptide-HLA-A*02:01 complexes according to the identity of the peptide. The facilitation was also specific for the identity of the HLA-I heavy chain, where it correlated to established tapasin dependence hierarchies. Two large sets of HLA-A*02:01 binding peptides, one extracted from natural HLA-I ligands from the SYFPEITHI database and one consisting of medium to high affinity non-SYFPEITHI ligands, were studied in the context of HLA-A*02:01 binding and stability. We show that the SYFPEITHI peptides induced more stable HLA-A*02:01 molecules than the other ligands, although affinities were similar. Remarkably, Tpn(1-87) could functionally discriminate the selected SYFPEITHI peptides from the other peptide binders with high sensitivity and specificity. We suggest that this HLA-I- and peptide-specific function, together with the functions exerted by the more C-terminal parts of tapasin, are major features of tapasin-mediated HLA-I quality control. These findings are important for understanding the biogenesis of HLA-I molecules, the selection of presented T-cell epitopes, and the identification of immunogenic targets in both basic research and vaccine design."xsd:string |
| http://purl.uniprot.org/citations/21518758 | http://purl.org/dc/terms/identifier | "doi:10.1074/jbc.m111.230151"xsd:string |
| http://purl.uniprot.org/citations/21518758 | http://purl.uniprot.org/core/author | "Rasmussen M."xsd:string |
| http://purl.uniprot.org/citations/21518758 | http://purl.uniprot.org/core/author | "Buus S."xsd:string |
| http://purl.uniprot.org/citations/21518758 | http://purl.uniprot.org/core/author | "Roder G."xsd:string |
| http://purl.uniprot.org/citations/21518758 | http://purl.uniprot.org/core/author | "Paulsson K."xsd:string |
| http://purl.uniprot.org/citations/21518758 | http://purl.uniprot.org/core/author | "Harndahl M."xsd:string |
| http://purl.uniprot.org/citations/21518758 | http://purl.uniprot.org/core/author | "Geironson L."xsd:string |
| http://purl.uniprot.org/citations/21518758 | http://purl.uniprot.org/core/date | "2011"xsd:gYear |
| http://purl.uniprot.org/citations/21518758 | http://purl.uniprot.org/core/name | "J Biol Chem"xsd:string |
| http://purl.uniprot.org/citations/21518758 | http://purl.uniprot.org/core/pages | "20547-20557"xsd:string |
| http://purl.uniprot.org/citations/21518758 | http://purl.uniprot.org/core/title | "Tapasin discriminates peptide-human leukocyte antigen-A*02:01 complexes formed with natural ligands."xsd:string |
| http://purl.uniprot.org/citations/21518758 | http://purl.uniprot.org/core/volume | "286"xsd:string |
| http://purl.uniprot.org/citations/21518758 | http://www.w3.org/2004/02/skos/core#exactMatch | http://purl.uniprot.org/pubmed/21518758 |
| http://purl.uniprot.org/citations/21518758 | http://xmlns.com/foaf/0.1/primaryTopicOf | https://pubmed.ncbi.nlm.nih.gov/21518758 |
| http://purl.uniprot.org/uniprot/#_A0A0A7C543-mappedCitation-21518758 | http://www.w3.org/1999/02/22-rdf-syntax-ns#object | http://purl.uniprot.org/citations/21518758 |
| http://purl.uniprot.org/uniprot/#_A0A0A7C548-mappedCitation-21518758 | http://www.w3.org/1999/02/22-rdf-syntax-ns#object | http://purl.uniprot.org/citations/21518758 |
| http://purl.uniprot.org/uniprot/#_A0A0A7C551-mappedCitation-21518758 | http://www.w3.org/1999/02/22-rdf-syntax-ns#object | http://purl.uniprot.org/citations/21518758 |
| http://purl.uniprot.org/uniprot/#_A0A0G2R0N3-mappedCitation-21518758 | http://www.w3.org/1999/02/22-rdf-syntax-ns#object | http://purl.uniprot.org/citations/21518758 |
| http://purl.uniprot.org/uniprot/#_A0A0G2R0N4-mappedCitation-21518758 | http://www.w3.org/1999/02/22-rdf-syntax-ns#object | http://purl.uniprot.org/citations/21518758 |
| http://purl.uniprot.org/uniprot/#_A0A076JXB7-mappedCitation-21518758 | http://www.w3.org/1999/02/22-rdf-syntax-ns#object | http://purl.uniprot.org/citations/21518758 |
| http://purl.uniprot.org/uniprot/#_A0A076L0P9-mappedCitation-21518758 | http://www.w3.org/1999/02/22-rdf-syntax-ns#object | http://purl.uniprot.org/citations/21518758 |
| http://purl.uniprot.org/uniprot/#_A0A0A0YLW6-mappedCitation-21518758 | http://www.w3.org/1999/02/22-rdf-syntax-ns#object | http://purl.uniprot.org/citations/21518758 |
| http://purl.uniprot.org/uniprot/#_A0A0A7C7I7-mappedCitation-21518758 | http://www.w3.org/1999/02/22-rdf-syntax-ns#object | http://purl.uniprot.org/citations/21518758 |