| http://purl.uniprot.org/citations/21518805 | http://www.w3.org/1999/02/22-rdf-syntax-ns#type | http://purl.uniprot.org/core/Journal_Citation |
| http://purl.uniprot.org/citations/21518805 | http://www.w3.org/1999/02/22-rdf-syntax-ns#type | http://purl.uniprot.org/core/Journal_Citation |
| http://purl.uniprot.org/citations/21518805 | http://www.w3.org/1999/02/22-rdf-syntax-ns#type | http://purl.uniprot.org/core/Citation |
| http://purl.uniprot.org/citations/21518805 | http://www.w3.org/2000/01/rdf-schema#comment | "Transfer RNAs contain various modified nucleotides that are introduced enzymatically at the post-transcriptional level. In Saccharomyces cerevisiae, 3-methylcytidine (m³C) is found at position 32 of the tRNAs for Thr and Ser. We used a systematic reverse genetic approach combined with mass spectrometry (ribonucleome analysis), and identified the actin-binding protein ABP140 as the protein responsible for m³C formation in both tRNA(Thr1) and tRNA(Ser1). ABP140 consists of an N-terminal actin-binding sequence and a C-terminal S-adenosylmethionine (Ado-Met) binding motif. Deletion of the actin-binding sequence in ABP140 did not affect m³C formation, indicating that subcellular localization of ABP140 to actin filaments is not involved in tRNA modification. m³C formation in tRNA(Thr1) could be reconstituted using recombinant Abp140p in the presence of Ado-Met, whereas m³C did not form in tRNA(Ser1) in vitro, indicating the absence of a factor(s) required for tRNA(Ser1) m³C formation. Thus, ABP140 has been designated TRM140 according to the preferred nomenclature. In addition, we observed a specific reduction of m³C formation in HeLa cells by siRNA-mediated knock down of the human ortholog of TRM140."xsd:string |
| http://purl.uniprot.org/citations/21518805 | http://purl.org/dc/terms/identifier | "doi:10.1261/rna.2653411"xsd:string |
| http://purl.uniprot.org/citations/21518805 | http://purl.org/dc/terms/identifier | "doi:10.1261/rna.2653411"xsd:string |
| http://purl.uniprot.org/citations/21518805 | http://purl.uniprot.org/core/author | "Katoh T."xsd:string |
| http://purl.uniprot.org/citations/21518805 | http://purl.uniprot.org/core/author | "Katoh T."xsd:string |
| http://purl.uniprot.org/citations/21518805 | http://purl.uniprot.org/core/author | "Takai Y."xsd:string |
| http://purl.uniprot.org/citations/21518805 | http://purl.uniprot.org/core/author | "Takai Y."xsd:string |
| http://purl.uniprot.org/citations/21518805 | http://purl.uniprot.org/core/author | "Suzuki T."xsd:string |
| http://purl.uniprot.org/citations/21518805 | http://purl.uniprot.org/core/author | "Suzuki T."xsd:string |
| http://purl.uniprot.org/citations/21518805 | http://purl.uniprot.org/core/author | "Yi S."xsd:string |
| http://purl.uniprot.org/citations/21518805 | http://purl.uniprot.org/core/author | "Yi S."xsd:string |
| http://purl.uniprot.org/citations/21518805 | http://purl.uniprot.org/core/author | "Noma A."xsd:string |
| http://purl.uniprot.org/citations/21518805 | http://purl.uniprot.org/core/author | "Noma A."xsd:string |
| http://purl.uniprot.org/citations/21518805 | http://purl.uniprot.org/core/author | "Suzuki T.'"xsd:string |
| http://purl.uniprot.org/citations/21518805 | http://purl.uniprot.org/core/author | "Suzuki T.'"xsd:string |
| http://purl.uniprot.org/citations/21518805 | http://purl.uniprot.org/core/date | "2011"xsd:gYear |
| http://purl.uniprot.org/citations/21518805 | http://purl.uniprot.org/core/date | "2011"xsd:gYear |
| http://purl.uniprot.org/citations/21518805 | http://purl.uniprot.org/core/name | "RNA"xsd:string |
| http://purl.uniprot.org/citations/21518805 | http://purl.uniprot.org/core/name | "RNA"xsd:string |
| http://purl.uniprot.org/citations/21518805 | http://purl.uniprot.org/core/pages | "1111-1119"xsd:string |
| http://purl.uniprot.org/citations/21518805 | http://purl.uniprot.org/core/pages | "1111-1119"xsd:string |
| http://purl.uniprot.org/citations/21518805 | http://purl.uniprot.org/core/title | "Actin-binding protein ABP140 is a methyltransferase for 3-methylcytidine at position 32 of tRNAs in Saccharomyces cerevisiae."xsd:string |