| http://purl.uniprot.org/citations/21536288 | http://www.w3.org/1999/02/22-rdf-syntax-ns#type | http://purl.uniprot.org/core/Journal_Citation |
| http://purl.uniprot.org/citations/21536288 | http://www.w3.org/2000/01/rdf-schema#comment | "Monocyte chemotactic protein-3 (MCP-3), also known as CCL7, belongs to the monocyte chemotactic protein (MCP) subfamily of the CC chemokines that includes MCP-1/CCL2, MCP-2/CCL8, MCP-4/CCL13, and MCP-5/CCL12. Few studies have examined the role of MCP-3 in vascular pathologies such as atherosclerosis and restenosis in which smooth muscle cell (SMC) proliferation plays an important role. In this study, we investigated the effect of MCP-3 on human coronary artery smooth muscle cell (CASMC) proliferation. MCP-3 induced concentration-dependent CASMC proliferation with the maximum stimulatory effect at 0.3 ng/mL (about 50% vs unstimulated cells) assessed by bromodeoxyuridine (BrdU) uptake and direct cell counting. Anti-MCP-3 antibody (20 ng/mL) completely inhibited cell proliferation, demonstrating the specificity of the proliferative effect of MCP-3. Moreover, the MCP-3-induced CASMC proliferation was blocked by RS 102895 (0.06-6 μM), a specific antagonist of chemokine receptor 2 (CCR2). The mitogenic effect of MCP-3 appeared to be dependent on ERK1/2 MAPK and PI3K signaling pathway activation, as demonstrated by the reduction of MCP-3-induced CASMC proliferation observed after the treatment of cells with U0126 (1 μM) and LY-294002 (5μM), selective inhibitors of ERK 1/2 and PI3K activation, respectively. We found no relationship between MCP-3-induced CASMC proliferation and nuclear factor-κB activation. Moreover, we found that tumor necrosis factor-α (TNF-α, 30 ng/mL) and interleukin-1β (IL-1β, 1 ng/mL) both induced time-dependent increase of MCP-3 production by CASMCs, which was reduced by the anti-MCP-3 antibody (20 ng/mL), suggesting that the mitogenic effect of these stimuli is due, at least in part, to MCP-3. In conclusion, our results demonstrate that MCP-3 is produced by human CASMCs and directly induces CASMC proliferation in vitro, suggesting a potential role for this chemokine in vascular pathology."xsd:string |
| http://purl.uniprot.org/citations/21536288 | http://purl.org/dc/terms/identifier | "doi:10.1016/j.atherosclerosis.2011.04.002"xsd:string |
| http://purl.uniprot.org/citations/21536288 | http://purl.uniprot.org/core/author | "Santamaria R."xsd:string |
| http://purl.uniprot.org/citations/21536288 | http://purl.uniprot.org/core/author | "Ialenti A."xsd:string |
| http://purl.uniprot.org/citations/21536288 | http://purl.uniprot.org/core/author | "Guglielmotti A."xsd:string |
| http://purl.uniprot.org/citations/21536288 | http://purl.uniprot.org/core/author | "Grassia G."xsd:string |
| http://purl.uniprot.org/citations/21536288 | http://purl.uniprot.org/core/author | "Maddaluno M."xsd:string |
| http://purl.uniprot.org/citations/21536288 | http://purl.uniprot.org/core/author | "Di Lauro M."xsd:string |
| http://purl.uniprot.org/citations/21536288 | http://purl.uniprot.org/core/author | "Di Pascale A."xsd:string |
| http://purl.uniprot.org/citations/21536288 | http://purl.uniprot.org/core/date | "2011"xsd:gYear |
| http://purl.uniprot.org/citations/21536288 | http://purl.uniprot.org/core/name | "Atherosclerosis"xsd:string |
| http://purl.uniprot.org/citations/21536288 | http://purl.uniprot.org/core/pages | "113-119"xsd:string |
| http://purl.uniprot.org/citations/21536288 | http://purl.uniprot.org/core/title | "Monocyte chemotactic protein-3 induces human coronary smooth muscle cell proliferation."xsd:string |
| http://purl.uniprot.org/citations/21536288 | http://purl.uniprot.org/core/volume | "217"xsd:string |
| http://purl.uniprot.org/citations/21536288 | http://www.w3.org/2004/02/skos/core#exactMatch | http://purl.uniprot.org/pubmed/21536288 |
| http://purl.uniprot.org/citations/21536288 | http://xmlns.com/foaf/0.1/primaryTopicOf | https://pubmed.ncbi.nlm.nih.gov/21536288 |
| http://purl.uniprot.org/uniprot/#_P80098-mappedCitation-21536288 | http://www.w3.org/1999/02/22-rdf-syntax-ns#object | http://purl.uniprot.org/citations/21536288 |
| http://purl.uniprot.org/uniprot/#_Q7Z7Q8-mappedCitation-21536288 | http://www.w3.org/1999/02/22-rdf-syntax-ns#object | http://purl.uniprot.org/citations/21536288 |
| http://purl.uniprot.org/uniprot/P80098 | http://purl.uniprot.org/core/mappedCitation | http://purl.uniprot.org/citations/21536288 |
| http://purl.uniprot.org/uniprot/Q7Z7Q8 | http://purl.uniprot.org/core/mappedCitation | http://purl.uniprot.org/citations/21536288 |