http://purl.uniprot.org/citations/21539897 | http://www.w3.org/1999/02/22-rdf-syntax-ns#type | http://purl.uniprot.org/core/Journal_Citation |
http://purl.uniprot.org/citations/21539897 | http://www.w3.org/1999/02/22-rdf-syntax-ns#type | http://purl.uniprot.org/core/Journal_Citation |
http://purl.uniprot.org/citations/21539897 | http://www.w3.org/2000/01/rdf-schema#comment | "BackgroundMultifunctional l-amino acid oxidases (LAAOs) occur widely in snake venoms.MethodsThe l-AAO from Bothrops leucurus (Bl-LAAO) venom was purified using a combination of molecular exclusion and ion-exchange chromatographies. We report some biochemical features of Bl-LAAO associated with its effect on platelet function and its cytotoxicity.ResultsBl-LAAO is a 60kDa monomeric glycoprotein. Its N-terminal sequence shows high homology to other members of the snake-venom LAAO family. Bl-LAAO catalyzes oxidative deamination of l-amino acids with the generation of H₂O₂. The best substrates were: l-Met, l-Norleu, l-Leu, l-Phe and l-Trp. The effects of snake venom LAAOs in hemostasis, especially their action on platelet function remain largely unknown. Bl-LAAO dose-dependently inhibited platelet aggregation of both human PRP and washed platelets. Moreover, the purified enzyme exhibited a killing effect in vitro against Leishmania sp., promastigotes, with a very low EC(50) of 0.07μM. Furthermore, the cytotoxicity of Bl-LAAO was observed in the stomach cancer MKN-45, adeno carcinoma HUTU, colorectal RKO and human fibroblast LL-24 cell lines. The enzyme released enough H₂O₂ in culture medium to induce apoptosis in cells in a dose- and time-dependent manner. The biological effects were inhibited by catalase.ConclusionBl-LAAO, a major component of B. leucurus venom, is a cytotoxin acting primarily via the generation of high amounts of H₂O₂ which kill the cells.General significanceThese results allow us to consider the use of LAAOs as anticancer agents, as tools in biochemical studies to investigate cellular processes, and to obtain a better understanding of the envenomation mechanism."xsd:string |
http://purl.uniprot.org/citations/21539897 | http://purl.org/dc/terms/identifier | "doi:10.1016/j.bbagen.2011.04.003"xsd:string |
http://purl.uniprot.org/citations/21539897 | http://purl.org/dc/terms/identifier | "doi:10.1016/j.bbagen.2011.04.003"xsd:string |
http://purl.uniprot.org/citations/21539897 | http://purl.uniprot.org/core/author | "Richardson M."xsd:string |
http://purl.uniprot.org/citations/21539897 | http://purl.uniprot.org/core/author | "Richardson M."xsd:string |
http://purl.uniprot.org/citations/21539897 | http://purl.uniprot.org/core/author | "Sanchez E.F."xsd:string |
http://purl.uniprot.org/citations/21539897 | http://purl.uniprot.org/core/author | "Sanchez E.F."xsd:string |
http://purl.uniprot.org/citations/21539897 | http://purl.uniprot.org/core/author | "Eble J.A."xsd:string |
http://purl.uniprot.org/citations/21539897 | http://purl.uniprot.org/core/author | "Eble J.A."xsd:string |
http://purl.uniprot.org/citations/21539897 | http://purl.uniprot.org/core/author | "Kohlhoff M."xsd:string |
http://purl.uniprot.org/citations/21539897 | http://purl.uniprot.org/core/author | "Kohlhoff M."xsd:string |
http://purl.uniprot.org/citations/21539897 | http://purl.uniprot.org/core/author | "Silva L."xsd:string |
http://purl.uniprot.org/citations/21539897 | http://purl.uniprot.org/core/author | "Silva L."xsd:string |
http://purl.uniprot.org/citations/21539897 | http://purl.uniprot.org/core/author | "Naumann G.B."xsd:string |
http://purl.uniprot.org/citations/21539897 | http://purl.uniprot.org/core/author | "Naumann G.B."xsd:string |
http://purl.uniprot.org/citations/21539897 | http://purl.uniprot.org/core/author | "Evangelista K."xsd:string |
http://purl.uniprot.org/citations/21539897 | http://purl.uniprot.org/core/author | "Evangelista K."xsd:string |
http://purl.uniprot.org/citations/21539897 | http://purl.uniprot.org/core/author | "Faria G."xsd:string |
http://purl.uniprot.org/citations/21539897 | http://purl.uniprot.org/core/author | "Faria G."xsd:string |
http://purl.uniprot.org/citations/21539897 | http://purl.uniprot.org/core/author | "Gontijo C.M."xsd:string |
http://purl.uniprot.org/citations/21539897 | http://purl.uniprot.org/core/author | "Gontijo C.M."xsd:string |
http://purl.uniprot.org/citations/21539897 | http://purl.uniprot.org/core/author | "Navdaev A."xsd:string |
http://purl.uniprot.org/citations/21539897 | http://purl.uniprot.org/core/author | "Navdaev A."xsd:string |