| http://purl.uniprot.org/citations/21542854 | http://www.w3.org/1999/02/22-rdf-syntax-ns#type | http://purl.uniprot.org/core/Journal_Citation |
| http://purl.uniprot.org/citations/21542854 | http://www.w3.org/1999/02/22-rdf-syntax-ns#type | http://purl.uniprot.org/core/Journal_Citation |
| http://purl.uniprot.org/citations/21542854 | http://www.w3.org/2000/01/rdf-schema#comment | "Gas vesicles are gas-filled protein structures increasing the buoyancy of cells. The gas vesicle envelope is mainly constituted by the 8 kDa protein GvpA forming a wall with a water excluding inner surface. A structure of GvpA is not available; recent solid-state NMR results suggest a coil-α-β-β-α-coil fold. We obtained a first structural model of GvpA by high-performance de novo modelling. Attenuated total reflection (ATR)-Fourier transform infrared spectroscopy (FTIR) supported this structure. A dimer of GvpA was derived that could explain the formation of the protein monolayer in the gas vesicle wall. The hydrophobic inner surface is mainly constituted by anti-parallel β-strands. The proposed structure allows the pinpointing of contact sites that were mutated and tested for the ability to form gas vesicles in haloarchaea. Mutations in α-helix I and α-helix II, but also in the β-turn affected the gas vesicle formation, whereas other alterations had no effect. All mutants supported the structural features deduced from the model. The proposed GvpA dimers allow the formation of a monolayer protein wall, also consistent with protease treatments of isolated gas vesicles."xsd:string |
| http://purl.uniprot.org/citations/21542854 | http://purl.org/dc/terms/identifier | "doi:10.1111/j.1365-2958.2011.07669.x"xsd:string |
| http://purl.uniprot.org/citations/21542854 | http://purl.org/dc/terms/identifier | "doi:10.1111/j.1365-2958.2011.07669.x"xsd:string |
| http://purl.uniprot.org/citations/21542854 | http://purl.uniprot.org/core/author | "Engelhardt H."xsd:string |
| http://purl.uniprot.org/citations/21542854 | http://purl.uniprot.org/core/author | "Engelhardt H."xsd:string |
| http://purl.uniprot.org/citations/21542854 | http://purl.uniprot.org/core/author | "Pfeifer F."xsd:string |
| http://purl.uniprot.org/citations/21542854 | http://purl.uniprot.org/core/author | "Pfeifer F."xsd:string |
| http://purl.uniprot.org/citations/21542854 | http://purl.uniprot.org/core/author | "Faist K."xsd:string |
| http://purl.uniprot.org/citations/21542854 | http://purl.uniprot.org/core/author | "Faist K."xsd:string |
| http://purl.uniprot.org/citations/21542854 | http://purl.uniprot.org/core/author | "Hamacher K."xsd:string |
| http://purl.uniprot.org/citations/21542854 | http://purl.uniprot.org/core/author | "Hamacher K."xsd:string |
| http://purl.uniprot.org/citations/21542854 | http://purl.uniprot.org/core/author | "Hoffgaard F."xsd:string |
| http://purl.uniprot.org/citations/21542854 | http://purl.uniprot.org/core/author | "Hoffgaard F."xsd:string |
| http://purl.uniprot.org/citations/21542854 | http://purl.uniprot.org/core/author | "Strunk T."xsd:string |
| http://purl.uniprot.org/citations/21542854 | http://purl.uniprot.org/core/author | "Strunk T."xsd:string |
| http://purl.uniprot.org/citations/21542854 | http://purl.uniprot.org/core/author | "Wenzel W."xsd:string |
| http://purl.uniprot.org/citations/21542854 | http://purl.uniprot.org/core/author | "Wenzel W."xsd:string |
| http://purl.uniprot.org/citations/21542854 | http://purl.uniprot.org/core/author | "Zillig M.D."xsd:string |
| http://purl.uniprot.org/citations/21542854 | http://purl.uniprot.org/core/author | "Zillig M.D."xsd:string |
| http://purl.uniprot.org/citations/21542854 | http://purl.uniprot.org/core/date | "2011"xsd:gYear |
| http://purl.uniprot.org/citations/21542854 | http://purl.uniprot.org/core/date | "2011"xsd:gYear |
| http://purl.uniprot.org/citations/21542854 | http://purl.uniprot.org/core/name | "Mol. Microbiol."xsd:string |
| http://purl.uniprot.org/citations/21542854 | http://purl.uniprot.org/core/name | "Mol. Microbiol."xsd:string |