| http://purl.uniprot.org/citations/21543267 | http://www.w3.org/1999/02/22-rdf-syntax-ns#type | http://purl.uniprot.org/core/Journal_Citation |
| http://purl.uniprot.org/citations/21543267 | http://www.w3.org/2000/01/rdf-schema#comment | "Meiotic homologous recombination in Saccharomyces cerevisiae involves formation of nucleoprotein filaments of Rad51 and Dmc1 that mediate DNA strand exchange between homologous chromosomes. The Mei5-Sae3 protein complex functions as a recombination mediator to promote nucleation of the Dmc1 recombinase onto replication protein A-coated single-stranded DNA. Here, we have expressed and purified the Mei5 protein, Sae3 protein and the Mei5-Sae3 complex for biochemical studies. We show the Mei5-Sae3 complex preferentially binds a fork-like DNA substrate to 3' overhanging DNA, single-stranded DNA or double-stranded DNA. We demonstrate that Mei5 confers DNA binding activity to the Mei5-Sae3 complex. We determined Mei5-Sae3 interacts with the Rad51 recombinase through the N-terminal domain of Mei5. Unlike Rad52, Mei5-Sae3 lacks recombination mediator activity for Rad51. Importantly, we find that the Mei5-Sae3 complex does not harbor single-strand DNA annealing activity. These properties of the Mei5-Sae3 complex distinguishes it from the Rad52 protein, which serves as the mediator of Rad51 and is involved in the single-strand DNA annealing pathway of homologous recombination."xsd:string |
| http://purl.uniprot.org/citations/21543267 | http://purl.org/dc/terms/identifier | "doi:10.1016/j.dnarep.2011.03.006"xsd:string |
| http://purl.uniprot.org/citations/21543267 | http://purl.uniprot.org/core/author | "Singh A.K."xsd:string |
| http://purl.uniprot.org/citations/21543267 | http://purl.uniprot.org/core/author | "Sharma D."xsd:string |
| http://purl.uniprot.org/citations/21543267 | http://purl.uniprot.org/core/author | "Sung P."xsd:string |
| http://purl.uniprot.org/citations/21543267 | http://purl.uniprot.org/core/author | "Tsubouchi H."xsd:string |
| http://purl.uniprot.org/citations/21543267 | http://purl.uniprot.org/core/author | "Sehorn M.G."xsd:string |
| http://purl.uniprot.org/citations/21543267 | http://purl.uniprot.org/core/author | "Leung W.K."xsd:string |
| http://purl.uniprot.org/citations/21543267 | http://purl.uniprot.org/core/author | "Ledford L.L."xsd:string |
| http://purl.uniprot.org/citations/21543267 | http://purl.uniprot.org/core/author | "Say A.F."xsd:string |
| http://purl.uniprot.org/citations/21543267 | http://purl.uniprot.org/core/author | "Sehorn H.A."xsd:string |
| http://purl.uniprot.org/citations/21543267 | http://purl.uniprot.org/core/date | "2011"xsd:gYear |
| http://purl.uniprot.org/citations/21543267 | http://purl.uniprot.org/core/name | "DNA Repair (Amst)"xsd:string |
| http://purl.uniprot.org/citations/21543267 | http://purl.uniprot.org/core/pages | "586-594"xsd:string |
| http://purl.uniprot.org/citations/21543267 | http://purl.uniprot.org/core/title | "The budding yeast Mei5-Sae3 complex interacts with Rad51 and preferentially binds a DNA fork structure."xsd:string |
| http://purl.uniprot.org/citations/21543267 | http://purl.uniprot.org/core/volume | "10"xsd:string |
| http://purl.uniprot.org/citations/21543267 | http://www.w3.org/2004/02/skos/core#exactMatch | http://purl.uniprot.org/pubmed/21543267 |
| http://purl.uniprot.org/citations/21543267 | http://xmlns.com/foaf/0.1/primaryTopicOf | https://pubmed.ncbi.nlm.nih.gov/21543267 |
| http://purl.uniprot.org/uniprot/#_A0A8H8ULW3-mappedCitation-21543267 | http://www.w3.org/1999/02/22-rdf-syntax-ns#object | http://purl.uniprot.org/citations/21543267 |
| http://purl.uniprot.org/uniprot/#_A0A6A5Q4Y7-mappedCitation-21543267 | http://www.w3.org/1999/02/22-rdf-syntax-ns#object | http://purl.uniprot.org/citations/21543267 |
| http://purl.uniprot.org/uniprot/#_P89114-mappedCitation-21543267 | http://www.w3.org/1999/02/22-rdf-syntax-ns#object | http://purl.uniprot.org/citations/21543267 |
| http://purl.uniprot.org/uniprot/#_P32489-mappedCitation-21543267 | http://www.w3.org/1999/02/22-rdf-syntax-ns#object | http://purl.uniprot.org/citations/21543267 |
| http://purl.uniprot.org/uniprot/#_P25454-mappedCitation-21543267 | http://www.w3.org/1999/02/22-rdf-syntax-ns#object | http://purl.uniprot.org/citations/21543267 |
| http://purl.uniprot.org/uniprot/A0A8H8ULW3 | http://purl.uniprot.org/core/mappedCitation | http://purl.uniprot.org/citations/21543267 |