Results

RDF/XMLNTriplesTurtleShow queryShare ▶
SubjectPredicateObject
http://purl.uniprot.org/citations/21548205http://www.w3.org/1999/02/22-rdf-syntax-ns#typehttp://purl.uniprot.org/core/Journal_Citation
http://purl.uniprot.org/citations/21548205http://www.w3.org/2000/01/rdf-schema#comment"

Objective

To investigate the influence of stromal cells on the Kallikrein 7 (KLK7) expression of epithelial cells in benign prostate hyperplasia (BPH).

Methods

We constructed a stromal-epithelial co-culture model after separating the two types of cells from BPH tissues and identifying them by cell morphology and chemiluminescent microparticle immunoassay (CMIA). The expression of KLK7 mRNA was detected by RT-PCR in the epithelial cells with or without the stromal cells, and that of the KLK7 protein (hK7) determined by Western blot.

Results

Stromal and epithelial cells were successfully separated and identified, and a stromal-epithelial co-culture model successfully established. RT-PCR showed that the mRNA expression of the KLK7 gene was higher in the epithelial cells co-cultured with stromal cells than in the epithelial cells alone, and the gray value of KLK7 to GAPDH was 1.41 +/-0.041 in the former and 1.78 +/-0.10 in the latter (P < 0.01). The results of Western blot were consistent with those of RT-PCR.

Conclusion

Stromal cells can suppress the expression of the KLK7 gene in the epithelial cells in BPH. KLK7 may be involved in the change of epithelial cells stimulated by stromal cells."xsd:string
http://purl.uniprot.org/citations/21548205http://purl.uniprot.org/core/author"Huang F.Y."xsd:string
http://purl.uniprot.org/citations/21548205http://purl.uniprot.org/core/author"Yang X.L."xsd:string
http://purl.uniprot.org/citations/21548205http://purl.uniprot.org/core/author"Pang Y.H."xsd:string
http://purl.uniprot.org/citations/21548205http://purl.uniprot.org/core/author"Mo Z.N."xsd:string
http://purl.uniprot.org/citations/21548205http://purl.uniprot.org/core/author"Xuan Q."xsd:string
http://purl.uniprot.org/citations/21548205http://purl.uniprot.org/core/date"2011"xsd:gYear
http://purl.uniprot.org/citations/21548205http://purl.uniprot.org/core/name"Zhonghua Nan Ke Xue"xsd:string
http://purl.uniprot.org/citations/21548205http://purl.uniprot.org/core/pages"305-309"xsd:string
http://purl.uniprot.org/citations/21548205http://purl.uniprot.org/core/title"[Stromal cells inhibit Kallikrein 7 expression in the epithelial cells of benign prostate hyperplasia]."xsd:string
http://purl.uniprot.org/citations/21548205http://purl.uniprot.org/core/volume"17"xsd:string
http://purl.uniprot.org/citations/21548205http://www.w3.org/2004/02/skos/core#exactMatchhttp://purl.uniprot.org/pubmed/21548205
http://purl.uniprot.org/citations/21548205http://xmlns.com/foaf/0.1/primaryTopicOfhttps://pubmed.ncbi.nlm.nih.gov/21548205
http://purl.uniprot.org/uniprot/#_A0A024R4H6-mappedCitation-21548205http://www.w3.org/1999/02/22-rdf-syntax-ns#objecthttp://purl.uniprot.org/citations/21548205
http://purl.uniprot.org/uniprot/#_A0A2H4GDB2-mappedCitation-21548205http://www.w3.org/1999/02/22-rdf-syntax-ns#objecthttp://purl.uniprot.org/citations/21548205
http://purl.uniprot.org/uniprot/#_A0A2H4GDB6-mappedCitation-21548205http://www.w3.org/1999/02/22-rdf-syntax-ns#objecthttp://purl.uniprot.org/citations/21548205
http://purl.uniprot.org/uniprot/#_B4DHX9-mappedCitation-21548205http://www.w3.org/1999/02/22-rdf-syntax-ns#objecthttp://purl.uniprot.org/citations/21548205
http://purl.uniprot.org/uniprot/#_B8K1J4-mappedCitation-21548205http://www.w3.org/1999/02/22-rdf-syntax-ns#objecthttp://purl.uniprot.org/citations/21548205
http://purl.uniprot.org/uniprot/#_P49862-mappedCitation-21548205http://www.w3.org/1999/02/22-rdf-syntax-ns#objecthttp://purl.uniprot.org/citations/21548205
http://purl.uniprot.org/uniprot/#_Q6DTY1-mappedCitation-21548205http://www.w3.org/1999/02/22-rdf-syntax-ns#objecthttp://purl.uniprot.org/citations/21548205
http://purl.uniprot.org/uniprot/#_X2J289-mappedCitation-21548205http://www.w3.org/1999/02/22-rdf-syntax-ns#objecthttp://purl.uniprot.org/citations/21548205
http://purl.uniprot.org/uniprot/#_X2J4X7-mappedCitation-21548205http://www.w3.org/1999/02/22-rdf-syntax-ns#objecthttp://purl.uniprot.org/citations/21548205
http://purl.uniprot.org/uniprot/A0A2H4GDB6http://purl.uniprot.org/core/mappedCitationhttp://purl.uniprot.org/citations/21548205
http://purl.uniprot.org/uniprot/B8K1J4http://purl.uniprot.org/core/mappedCitationhttp://purl.uniprot.org/citations/21548205
▶