| http://purl.uniprot.org/citations/21565799 | http://www.w3.org/1999/02/22-rdf-syntax-ns#type | http://purl.uniprot.org/core/Journal_Citation |
| http://purl.uniprot.org/citations/21565799 | http://www.w3.org/1999/02/22-rdf-syntax-ns#type | http://purl.uniprot.org/core/Journal_Citation |
| http://purl.uniprot.org/citations/21565799 | http://www.w3.org/2000/01/rdf-schema#comment | "The conjugative transfer of F-like plasmids such as F, R1, R100 and pED208, between bacterial cells requires TraM, a plasmid-encoded DNA-binding protein. TraM tetramers bridge the origin of transfer (oriT) to a key component of the conjugative pore, the coupling protein TraD. Here we show that TraM recognizes a high-affinity DNA-binding site, sbmA, as a cooperative dimer of tetramers. The crystal structure of the TraM-sbmA complex from the plasmid pED208 shows that binding cooperativity is mediated by DNA kinking and unwinding, without any direct contact between tetramers. Sequence-specific DNA recognition is carried out by TraM's N-terminal ribbon-helix-helix (RHH) domains, which bind DNA in a staggered arrangement. We demonstrate that both DNA-binding specificity, as well as selective interactions between TraM and the C-terminal tail of its cognate TraD mediate conjugation specificity within the F-like family of plasmids. The ability of TraM to cooperatively bind DNA without interaction between tetramers leaves the C-terminal TraM tetramerization domains free to make multiple interactions with TraD, driving recruitment of the plasmid to the conjugative pore."xsd:string |
| http://purl.uniprot.org/citations/21565799 | http://purl.org/dc/terms/identifier | "doi:10.1093/nar/gkr296"xsd:string |
| http://purl.uniprot.org/citations/21565799 | http://purl.org/dc/terms/identifier | "doi:10.1093/nar/gkr296"xsd:string |
| http://purl.uniprot.org/citations/21565799 | http://purl.uniprot.org/core/author | "Lu J."xsd:string |
| http://purl.uniprot.org/citations/21565799 | http://purl.uniprot.org/core/author | "Lu J."xsd:string |
| http://purl.uniprot.org/citations/21565799 | http://purl.uniprot.org/core/author | "Wong J.J."xsd:string |
| http://purl.uniprot.org/citations/21565799 | http://purl.uniprot.org/core/author | "Wong J.J."xsd:string |
| http://purl.uniprot.org/citations/21565799 | http://purl.uniprot.org/core/author | "Frost L.S."xsd:string |
| http://purl.uniprot.org/citations/21565799 | http://purl.uniprot.org/core/author | "Frost L.S."xsd:string |
| http://purl.uniprot.org/citations/21565799 | http://purl.uniprot.org/core/author | "Edwards R.A."xsd:string |
| http://purl.uniprot.org/citations/21565799 | http://purl.uniprot.org/core/author | "Edwards R.A."xsd:string |
| http://purl.uniprot.org/citations/21565799 | http://purl.uniprot.org/core/author | "Glover J.N."xsd:string |
| http://purl.uniprot.org/citations/21565799 | http://purl.uniprot.org/core/author | "Glover J.N."xsd:string |
| http://purl.uniprot.org/citations/21565799 | http://purl.uniprot.org/core/date | "2011"xsd:gYear |
| http://purl.uniprot.org/citations/21565799 | http://purl.uniprot.org/core/date | "2011"xsd:gYear |
| http://purl.uniprot.org/citations/21565799 | http://purl.uniprot.org/core/name | "Nucleic Acids Res."xsd:string |
| http://purl.uniprot.org/citations/21565799 | http://purl.uniprot.org/core/name | "Nucleic Acids Res."xsd:string |
| http://purl.uniprot.org/citations/21565799 | http://purl.uniprot.org/core/pages | "6775-6788"xsd:string |
| http://purl.uniprot.org/citations/21565799 | http://purl.uniprot.org/core/pages | "6775-6788"xsd:string |
| http://purl.uniprot.org/citations/21565799 | http://purl.uniprot.org/core/title | "Structural basis of cooperative DNA recognition by the plasmid conjugation factor, TraM."xsd:string |
| http://purl.uniprot.org/citations/21565799 | http://purl.uniprot.org/core/title | "Structural basis of cooperative DNA recognition by the plasmid conjugation factor, TraM."xsd:string |
| http://purl.uniprot.org/citations/21565799 | http://purl.uniprot.org/core/volume | "39"xsd:string |
| http://purl.uniprot.org/citations/21565799 | http://purl.uniprot.org/core/volume | "39"xsd:string |