| http://purl.uniprot.org/citations/2156852 | http://www.w3.org/1999/02/22-rdf-syntax-ns#type | http://purl.uniprot.org/core/Journal_Citation |
| http://purl.uniprot.org/citations/2156852 | http://www.w3.org/1999/02/22-rdf-syntax-ns#type | http://purl.uniprot.org/core/Journal_Citation |
| http://purl.uniprot.org/citations/2156852 | http://www.w3.org/2000/01/rdf-schema#comment | "The receptor for human urokinase-type plasminogen activator (u-PA) was purified from phorbol 12-myristate 13-acetate-stimulated U937 cells by temperature-induced phase separation of detergent extracts, followed by affinity chromatography with immobilized diisopropyl fluorophosphate-treated u-PA. The purified protein shows a single 55-60 kDa band after sodium dodecyl sulfate-polyacrylamide gel electrophoresis and silver staining. It is a heavily glycosylated protein, the deglycosylated polypeptide chain comprising only 35 kDa. The glycosylated protein contains N-acetyl-D-glucosamine and sialic acid, but no N-acetyl-D-galactosamine. Glycosylation is responsible for substantial heterogeneity in the receptor on phorbol ester-stimulated U937 cells, and also for molecular weight variations among various cell lines. The amino acid composition and the NH2-terminal amino acid sequence are reported. The protein has a high content of cysteine residues. The NH2-terminal sequence is not closely related to any known sequence. The identification of the purified and sequenced protein with the human u-PA receptor is based on the following findings: 1) the ability of the purified protein to bind u-PA and its amino-terminal fragment; 2) the identical electrophoretic mobilities observed for cross-linked conjugates, formed between either the purified protein or the u-PA receptor on intact U937 cells and the above ligands; 3) the identity of the apparent molecular weight of the purified protein to that predicted for the u-PA receptor in the same cross-linking studies; 4) the identical extent of glycosylation of the purified protein and of the u-PA receptor in crude membrane fractions, as detected after cross-linking; 5) the ability of antibodies raised against the purified protein to inhibit cellular binding of the amino-terminal fragment of u-PA."xsd:string |
| http://purl.uniprot.org/citations/2156852 | http://purl.org/dc/terms/identifier | "doi:10.1016/s0021-9258(19)39348-2"xsd:string |
| http://purl.uniprot.org/citations/2156852 | http://purl.org/dc/terms/identifier | "doi:10.1016/s0021-9258(19)39348-2"xsd:string |
| http://purl.uniprot.org/citations/2156852 | http://purl.uniprot.org/core/author | "Appella E."xsd:string |
| http://purl.uniprot.org/citations/2156852 | http://purl.uniprot.org/core/author | "Appella E."xsd:string |
| http://purl.uniprot.org/citations/2156852 | http://purl.uniprot.org/core/author | "Blasi F."xsd:string |
| http://purl.uniprot.org/citations/2156852 | http://purl.uniprot.org/core/author | "Blasi F."xsd:string |
| http://purl.uniprot.org/citations/2156852 | http://purl.uniprot.org/core/author | "Ploug M."xsd:string |
| http://purl.uniprot.org/citations/2156852 | http://purl.uniprot.org/core/author | "Ploug M."xsd:string |
| http://purl.uniprot.org/citations/2156852 | http://purl.uniprot.org/core/author | "Behrendt N."xsd:string |
| http://purl.uniprot.org/citations/2156852 | http://purl.uniprot.org/core/author | "Behrendt N."xsd:string |
| http://purl.uniprot.org/citations/2156852 | http://purl.uniprot.org/core/author | "Schleuning W.-D."xsd:string |
| http://purl.uniprot.org/citations/2156852 | http://purl.uniprot.org/core/author | "Schleuning W.-D."xsd:string |
| http://purl.uniprot.org/citations/2156852 | http://purl.uniprot.org/core/author | "Danoe K."xsd:string |
| http://purl.uniprot.org/citations/2156852 | http://purl.uniprot.org/core/author | "Danoe K."xsd:string |
| http://purl.uniprot.org/citations/2156852 | http://purl.uniprot.org/core/author | "Nielsen L.S."xsd:string |
| http://purl.uniprot.org/citations/2156852 | http://purl.uniprot.org/core/author | "Nielsen L.S."xsd:string |
| http://purl.uniprot.org/citations/2156852 | http://purl.uniprot.org/core/author | "Roenne E."xsd:string |
| http://purl.uniprot.org/citations/2156852 | http://purl.uniprot.org/core/author | "Roenne E."xsd:string |
| http://purl.uniprot.org/citations/2156852 | http://purl.uniprot.org/core/author | "Loeber D."xsd:string |
| http://purl.uniprot.org/citations/2156852 | http://purl.uniprot.org/core/author | "Loeber D."xsd:string |
| http://purl.uniprot.org/citations/2156852 | http://purl.uniprot.org/core/author | "Petri T."xsd:string |
| http://purl.uniprot.org/citations/2156852 | http://purl.uniprot.org/core/author | "Petri T."xsd:string |