Results

RDF/XMLNTriplesTurtleShow queryShare ▶
SubjectPredicateObject
http://purl.uniprot.org/citations/21569040http://www.w3.org/1999/02/22-rdf-syntax-ns#typehttp://purl.uniprot.org/core/Journal_Citation
http://purl.uniprot.org/citations/21569040http://www.w3.org/1999/02/22-rdf-syntax-ns#typehttp://purl.uniprot.org/core/Journal_Citation
http://purl.uniprot.org/citations/21569040http://www.w3.org/2000/01/rdf-schema#comment"

Background

Avoiding immunization with clinically important antibodies is a primary objective in transfusion medicine. Therefore, it is central to identify the extent of D antigens that escape routine RhD typing of blood donors and to improve methodology if necessary.

Study design and methods

We screened 5058 D-donors for the presence of the RHD gene, targeting Exons 5, 7, and 10 with real-time polymerase chain reaction. Samples that were positive in the screen test were investigated further by adsorption-elution, antibody consumption, flow cytometry, and sequencing of all RHD exons with intron-specific primers. Lookback was performed on all recipients of RBCs from RHD+ donors.

Results

We found 13 RHD+ samples (0.26%). No variants or chimeras were found. Characterization of DNA revealed a novel DEL type (IVS2-2 A>G). In the lookback of the 136 transfusions with subsequent antibody follow-up, of which 13 were from DEL donors, one recipient developed anti-D. However, in this case, a competing and more likely cause of immunization was the concurrent transfusion of D+ platelets. Eleven recipients were immunized with 13 antibodies different from anti-D, of which five were anti-K.

Conclusion

In our laboratory, serologic RhD typing was safe. We detected all D variants and only missed DEL types. In assessing the immunization risk we included a DEL donor, found previous to this study, that did immunize a recipient with anti-D. We conclude that inadvertent immunization with D antigens in our setting was rare and in the order of 1.4 in 100,000 D- transfusions."xsd:string
http://purl.uniprot.org/citations/21569040http://purl.org/dc/terms/identifier"doi:10.1111/j.1537-2995.2011.03156.x"xsd:string
http://purl.uniprot.org/citations/21569040http://purl.org/dc/terms/identifier"doi:10.1111/j.1537-2995.2011.03156.x"xsd:string
http://purl.uniprot.org/citations/21569040http://purl.uniprot.org/core/author"Jorgensen L."xsd:string
http://purl.uniprot.org/citations/21569040http://purl.uniprot.org/core/author"Jorgensen L."xsd:string
http://purl.uniprot.org/citations/21569040http://purl.uniprot.org/core/author"Nielsen L.K."xsd:string
http://purl.uniprot.org/citations/21569040http://purl.uniprot.org/core/author"Nielsen L.K."xsd:string
http://purl.uniprot.org/citations/21569040http://purl.uniprot.org/core/author"Rieneck K."xsd:string
http://purl.uniprot.org/citations/21569040http://purl.uniprot.org/core/author"Rieneck K."xsd:string
http://purl.uniprot.org/citations/21569040http://purl.uniprot.org/core/author"Dziegiel M.H."xsd:string
http://purl.uniprot.org/citations/21569040http://purl.uniprot.org/core/author"Dziegiel M.H."xsd:string
http://purl.uniprot.org/citations/21569040http://purl.uniprot.org/core/author"Clausen F.B."xsd:string
http://purl.uniprot.org/citations/21569040http://purl.uniprot.org/core/author"Clausen F.B."xsd:string
http://purl.uniprot.org/citations/21569040http://purl.uniprot.org/core/author"Krog G.R."xsd:string
http://purl.uniprot.org/citations/21569040http://purl.uniprot.org/core/author"Krog G.R."xsd:string
http://purl.uniprot.org/citations/21569040http://purl.uniprot.org/core/author"Berkowicz A."xsd:string
http://purl.uniprot.org/citations/21569040http://purl.uniprot.org/core/author"Berkowicz A."xsd:string
http://purl.uniprot.org/citations/21569040http://purl.uniprot.org/core/date"2011"xsd:gYear
http://purl.uniprot.org/citations/21569040http://purl.uniprot.org/core/date"2011"xsd:gYear
http://purl.uniprot.org/citations/21569040http://purl.uniprot.org/core/name"Transfusion"xsd:string
http://purl.uniprot.org/citations/21569040http://purl.uniprot.org/core/name"Transfusion"xsd:string
http://purl.uniprot.org/citations/21569040http://purl.uniprot.org/core/pages"2278-2285"xsd:string
http://purl.uniprot.org/citations/21569040http://purl.uniprot.org/core/pages"2278-2285"xsd:string
▶