| http://purl.uniprot.org/citations/2157703 | http://www.w3.org/1999/02/22-rdf-syntax-ns#type | http://purl.uniprot.org/core/Journal_Citation |
| http://purl.uniprot.org/citations/2157703 | http://www.w3.org/2000/01/rdf-schema#comment | "The 5'(3')-nucleotidase earlier partially purified from rat liver by Fritzson and Smith [1971) Biochim. Biophys. Acta 235, 128-141) was purified 15,000-fold to apparent homogeneity from human placenta. The soluble enzyme is a homodimer with a native molecular mass of 44-45 kDa. It has a pH optimum between 6.0 and 6.5 and is absolutely dependent on Mg2+ ions. The enzyme dephosphorylates certain 2'-, 3'-, and 5'-nucleotides. Km values for 2'- and 3'-nucleotides are around 0.3 mM with no preference for either ribo- or deoxyribonucleotides. 5'-Deoxyribonucleotides are 10-fold better substrates than the corresponding ribonucleotides, with dIMP greater than dUMP greater than dGMP greater than dTMP. dAMP is a poor substrate, dCMP is essentially insert. In all cases the Km values are in the millimolar range. Of the different forms of nucleotidases characterized in animal cells, the 5'(3')-nucleotidase is unique in its preference for 5'-deoxyribonucleotides. In intact cells, a portion of de novo synthesized deoxyribonucleotides is degraded as part of a homeostatic mechanism regulating the size of deoxyribonucleotide pools. This requires the participation of one or several 5'-nucleotidases. The 5'(3')-nucleotidase may be one such enzyme."xsd:string |
| http://purl.uniprot.org/citations/2157703 | http://purl.org/dc/terms/identifier | "doi:10.1016/s0021-9258(19)39188-4"xsd:string |
| http://purl.uniprot.org/citations/2157703 | http://purl.uniprot.org/core/author | "Reichard P."xsd:string |
| http://purl.uniprot.org/citations/2157703 | http://purl.uniprot.org/core/author | "Hoglund L."xsd:string |
| http://purl.uniprot.org/citations/2157703 | http://purl.uniprot.org/core/date | "1990"xsd:gYear |
| http://purl.uniprot.org/citations/2157703 | http://purl.uniprot.org/core/name | "J Biol Chem"xsd:string |
| http://purl.uniprot.org/citations/2157703 | http://purl.uniprot.org/core/pages | "6589-6595"xsd:string |
| http://purl.uniprot.org/citations/2157703 | http://purl.uniprot.org/core/title | "Cytoplasmic 5'(3')-nucleotidase from human placenta."xsd:string |
| http://purl.uniprot.org/citations/2157703 | http://purl.uniprot.org/core/volume | "265"xsd:string |
| http://purl.uniprot.org/citations/2157703 | http://www.w3.org/2004/02/skos/core#exactMatch | http://purl.uniprot.org/pubmed/2157703 |
| http://purl.uniprot.org/citations/2157703 | http://xmlns.com/foaf/0.1/primaryTopicOf | https://pubmed.ncbi.nlm.nih.gov/2157703 |
| http://purl.uniprot.org/uniprot/#_Q8TCD5-mappedCitation-2157703 | http://www.w3.org/1999/02/22-rdf-syntax-ns#object | http://purl.uniprot.org/citations/2157703 |
| http://purl.uniprot.org/uniprot/Q8TCD5 | http://purl.uniprot.org/core/mappedCitation | http://purl.uniprot.org/citations/2157703 |