| http://purl.uniprot.org/citations/21593607 | http://www.w3.org/1999/02/22-rdf-syntax-ns#type | http://purl.uniprot.org/core/Journal_Citation |
| http://purl.uniprot.org/citations/21593607 | http://www.w3.org/1999/02/22-rdf-syntax-ns#type | http://purl.uniprot.org/core/Journal_Citation |
| http://purl.uniprot.org/citations/21593607 | http://www.w3.org/2000/01/rdf-schema#comment | "Accurate tRNA processing is crucial for human mitochondrial genome expression, but the mechanisms of mt-tRNA cleavage and the key enzymes involved in this process are poorly characterized. At least two activities are required for proper mt-tRNA maturation: RNase P cleaving precursor molecules at the 5' end and tRNase Z at the 3' end. In human mitochondria only RNase P has been identified so far. Using RT-PCR and northern blot analyses we found that silencing of the human ELAC2 gene results in impaired 3' end of mt-tRNAs. We demonstrate this for several mitochondrial tRNAs, encoded on both mtDNA strands, including tRNA (Val) , tRNA (Lys) , tRNA (Arg) , tRNA (Gly) , tRNA (Leu(UUR)) and tRNA (Glu) . The silencing of the MRPP1 gene that encodes a subunit of mtRNase P resulted in inhibition of both 5' and 3' processing. We also demonstrate the double mitochondrial/nuclear localization of the ELAC2 protein using immunofluorescence. Our results indicate that ELAC2 functions as a tRNase Z in human mitochondria and suggest that mt-tRNase Z preferentially cleaves molecules already processed by the proteinaceous mtRNase P."xsd:string |
| http://purl.uniprot.org/citations/21593607 | http://purl.org/dc/terms/identifier | "doi:10.4161/rna.8.4.15393"xsd:string |
| http://purl.uniprot.org/citations/21593607 | http://purl.org/dc/terms/identifier | "doi:10.4161/rna.8.4.15393"xsd:string |
| http://purl.uniprot.org/citations/21593607 | http://purl.uniprot.org/core/author | "Stepien P.P."xsd:string |
| http://purl.uniprot.org/citations/21593607 | http://purl.uniprot.org/core/author | "Stepien P.P."xsd:string |
| http://purl.uniprot.org/citations/21593607 | http://purl.uniprot.org/core/author | "Szczesny R.J."xsd:string |
| http://purl.uniprot.org/citations/21593607 | http://purl.uniprot.org/core/author | "Szczesny R.J."xsd:string |
| http://purl.uniprot.org/citations/21593607 | http://purl.uniprot.org/core/author | "Bijata M."xsd:string |
| http://purl.uniprot.org/citations/21593607 | http://purl.uniprot.org/core/author | "Bijata M."xsd:string |
| http://purl.uniprot.org/citations/21593607 | http://purl.uniprot.org/core/author | "Brzezniak L.K."xsd:string |
| http://purl.uniprot.org/citations/21593607 | http://purl.uniprot.org/core/author | "Brzezniak L.K."xsd:string |
| http://purl.uniprot.org/citations/21593607 | http://purl.uniprot.org/core/date | "2011"xsd:gYear |
| http://purl.uniprot.org/citations/21593607 | http://purl.uniprot.org/core/date | "2011"xsd:gYear |
| http://purl.uniprot.org/citations/21593607 | http://purl.uniprot.org/core/name | "RNA Biol."xsd:string |
| http://purl.uniprot.org/citations/21593607 | http://purl.uniprot.org/core/name | "RNA Biol."xsd:string |
| http://purl.uniprot.org/citations/21593607 | http://purl.uniprot.org/core/pages | "616-626"xsd:string |
| http://purl.uniprot.org/citations/21593607 | http://purl.uniprot.org/core/pages | "616-626"xsd:string |
| http://purl.uniprot.org/citations/21593607 | http://purl.uniprot.org/core/title | "Involvement of human ELAC2 gene product in 3' end processing of mitochondrial tRNAs."xsd:string |
| http://purl.uniprot.org/citations/21593607 | http://purl.uniprot.org/core/title | "Involvement of human ELAC2 gene product in 3' end processing of mitochondrial tRNAs."xsd:string |
| http://purl.uniprot.org/citations/21593607 | http://purl.uniprot.org/core/volume | "8"xsd:string |
| http://purl.uniprot.org/citations/21593607 | http://purl.uniprot.org/core/volume | "8"xsd:string |
| http://purl.uniprot.org/citations/21593607 | http://www.w3.org/2004/02/skos/core#exactMatch | http://purl.uniprot.org/pubmed/21593607 |
| http://purl.uniprot.org/citations/21593607 | http://www.w3.org/2004/02/skos/core#exactMatch | http://purl.uniprot.org/pubmed/21593607 |