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http://purl.uniprot.org/citations/21602128http://www.w3.org/1999/02/22-rdf-syntax-ns#typehttp://purl.uniprot.org/core/Journal_Citation
http://purl.uniprot.org/citations/21602128http://www.w3.org/2000/01/rdf-schema#comment"

Objective

To investigate the mechanism of the development of cisplatin resistance in a human esophageal squamous carcinoma cell line.

Methods

The cytotoxicity of cisplatin in the cisplatin-resistant resistant cell line EC109/CDDP and its parental cell line EC109 was measured by MTT assay. Whole-cell cisplatin accumulation and Pt-DNA adduct formation were determined by inductively coupled plasma mass spectrometry (ICP-MS). Western blotting was used to investigate the protein expression of full length PARP, cleaved PARP, and copper transporter 1 (CTR1).

Results

EC109/CDDP cells was more resistant to cisplatin-induced cytotoxicity and apoptosis than EC109 cells. Compared with EC109 cells, EC109/CDDP cells exhibited less cisplatin accumulation and Pt-DNA adduct formation with also decreased CTR1 protein expression.

Conclusion

Cisplatin induces drug resistant phenotype by decreasing the protein level of CTR1, which controls cell accumulation and cytotoxic effect of cisplatin."xsd:string
http://purl.uniprot.org/citations/21602128http://purl.uniprot.org/core/author"Li Y.L."xsd:string
http://purl.uniprot.org/citations/21602128http://purl.uniprot.org/core/author"Yu L."xsd:string
http://purl.uniprot.org/citations/21602128http://purl.uniprot.org/core/author"Wen J."xsd:string
http://purl.uniprot.org/citations/21602128http://purl.uniprot.org/core/author"Cho C.H."xsd:string
http://purl.uniprot.org/citations/21602128http://purl.uniprot.org/core/author"Chen M.H."xsd:string
http://purl.uniprot.org/citations/21602128http://purl.uniprot.org/core/author"Liu S.W."xsd:string
http://purl.uniprot.org/citations/21602128http://purl.uniprot.org/core/author"Fu J.H."xsd:string
http://purl.uniprot.org/citations/21602128http://purl.uniprot.org/core/author"Gu C.P."xsd:string
http://purl.uniprot.org/citations/21602128http://purl.uniprot.org/core/date"2011"xsd:gYear
http://purl.uniprot.org/citations/21602128http://purl.uniprot.org/core/name"Nan Fang Yi Ke Da Xue Xue Bao"xsd:string
http://purl.uniprot.org/citations/21602128http://purl.uniprot.org/core/pages"801-804"xsd:string
http://purl.uniprot.org/citations/21602128http://purl.uniprot.org/core/title"[Cisplatin induces drug resistance in human esophageal squamous carcinoma cell line EC109 by decreasing CTR1 protein expression]."xsd:string
http://purl.uniprot.org/citations/21602128http://purl.uniprot.org/core/volume"31"xsd:string
http://purl.uniprot.org/citations/21602128http://www.w3.org/2004/02/skos/core#exactMatchhttp://purl.uniprot.org/pubmed/21602128
http://purl.uniprot.org/citations/21602128http://xmlns.com/foaf/0.1/primaryTopicOfhttps://pubmed.ncbi.nlm.nih.gov/21602128
http://purl.uniprot.org/uniprot/#_O15431-mappedCitation-21602128http://www.w3.org/1999/02/22-rdf-syntax-ns#objecthttp://purl.uniprot.org/citations/21602128
http://purl.uniprot.org/uniprot/#_Q9BT69-mappedCitation-21602128http://www.w3.org/1999/02/22-rdf-syntax-ns#objecthttp://purl.uniprot.org/citations/21602128
http://purl.uniprot.org/uniprot/#_Q6P708-mappedCitation-21602128http://www.w3.org/1999/02/22-rdf-syntax-ns#objecthttp://purl.uniprot.org/citations/21602128
http://purl.uniprot.org/uniprot/O15431http://purl.uniprot.org/core/mappedCitationhttp://purl.uniprot.org/citations/21602128
http://purl.uniprot.org/uniprot/Q9BT69http://purl.uniprot.org/core/mappedCitationhttp://purl.uniprot.org/citations/21602128
http://purl.uniprot.org/uniprot/Q6P708http://purl.uniprot.org/core/mappedCitationhttp://purl.uniprot.org/citations/21602128