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Background

During endocytosis in yeast, a choreographed series of discrete local events at the plasma membrane lead to a rapid burst of actin polymerization and the subsequent internalization of an endocytic vesicle. What initiates Arp2/3-dependent actin polymerization in this process is not well understood.

Results

The Schizosaccharomyces pombe WISH/DIP/SPIN90 ortholog dip1p is an actin-patch protein that regulates the temporal sequence of endocytic events. dip1Δ mutants exhibit a novel phenotype in which early events such as WASp localization occur normally but arrival of Arp2/3, actin polymerization, and subsequent steps are delayed and occur with apparently random timing. In studying this mutant, we demonstrate that positive feedback loops of WASp, rapid actin assembly, and Arp2/3 contribute to switch-like behavior that initiates actin polymerization. In the absence of dip1p, a subset of patches is activated concurrently with the "touch" of a neighboring endocytic vesicle.

Conclusions

These studies reveal a switch-like mechanism responsible for the initiation of actin assembly during endocytosis. This switch may be activated in at least two ways, through a dip1p-dependent mechanism and through contact with another endocytic vesicle."xsd:string
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http://purl.uniprot.org/citations/21620704http://purl.uniprot.org/core/author"Basu R."xsd:string
http://purl.uniprot.org/citations/21620704http://purl.uniprot.org/core/author"Chang F."xsd:string
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http://purl.uniprot.org/citations/21620704http://purl.uniprot.org/core/name"Curr Biol"xsd:string
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http://purl.uniprot.org/citations/21620704http://purl.uniprot.org/core/title"Characterization of dip1p reveals a switch in Arp2/3-dependent actin assembly for fission yeast endocytosis."xsd:string
http://purl.uniprot.org/citations/21620704http://purl.uniprot.org/core/volume"21"xsd:string
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