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http://purl.uniprot.org/citations/21624897http://www.w3.org/1999/02/22-rdf-syntax-ns#typehttp://purl.uniprot.org/core/Journal_Citation
http://purl.uniprot.org/citations/21624897http://www.w3.org/2000/01/rdf-schema#comment"It is widely considered that ADP-glucose pyrophosphorylase (AGP) is the sole source of ADP-glucose linked to bacterial glycogen and plant starch biosynthesis. Genetic evidence that bacterial glycogen biosynthesis occurs solely by the AGP pathway has been obtained with glgC⁻ AGP mutants. However, recent studies have shown that (i) these mutants can accumulate high levels of ADP-glucose and glycogen, and (ii) there are sources other than GlgC, of ADP-glucose linked to glycogen biosynthesis. In Arabidopsis, evidence showing that starch biosynthesis occurs solely by the AGP pathway has been obtained with the starchless adg1-1 and aps1 AGP mutants. However, mounting evidence has been compiled previewing the occurrence of more than one important ADP-glucose source in plants. In attempting to solve this 20-year-old controversy, in this work we carried out a judicious characterization of both adg1-1 and aps1. Both mutants accumulated wild-type (WT) ADP-glucose and approximately 2% of WT starch, as further confirmed by confocal fluorescence microscopic observation of iodine-stained leaves and of leaves expressing granule-bound starch synthase fused with GFP. Introduction of the sex1 mutation affecting starch breakdown into adg1-1 and aps1 increased the starch content to 8-10% of the WT starch. Furthermore, aps1 leaves exposed to microbial volatiles for 10 h accumulated approximately 60% of the WT starch. aps1 plants expressing the bacterial ADP-glucose hydrolase EcASPP in the plastid accumulated normal ADP-glucose and reduced starch when compared with aps1 plants, whereas aps1 plants expressing EcASPP in the cytosol showed reduced ADP-glucose and starch. Moreover, aps1 plants expressing bacterial AGP in the plastid accumulated WT starch and ADP-glucose. The overall data show that (i) there occur important source(s), other than AGP, of ADP-glucose linked to starch biosynthesis, and (ii) AGP is a major determinant of starch accumulation but not of intracellular ADP-glucose content in Arabidopsis."xsd:string
http://purl.uniprot.org/citations/21624897http://purl.org/dc/terms/identifier"doi:10.1093/pcp/pcr067"xsd:string
http://purl.uniprot.org/citations/21624897http://purl.uniprot.org/core/author"Li J."xsd:string
http://purl.uniprot.org/citations/21624897http://purl.uniprot.org/core/author"Baroja-Fernandez E."xsd:string
http://purl.uniprot.org/citations/21624897http://purl.uniprot.org/core/author"Munoz F.J."xsd:string
http://purl.uniprot.org/citations/21624897http://purl.uniprot.org/core/author"Pozueta-Romero J."xsd:string
http://purl.uniprot.org/citations/21624897http://purl.uniprot.org/core/author"Hidalgo M."xsd:string
http://purl.uniprot.org/citations/21624897http://purl.uniprot.org/core/author"Montero M."xsd:string
http://purl.uniprot.org/citations/21624897http://purl.uniprot.org/core/author"Romero J.M."xsd:string
http://purl.uniprot.org/citations/21624897http://purl.uniprot.org/core/author"Almagro G."xsd:string
http://purl.uniprot.org/citations/21624897http://purl.uniprot.org/core/author"Bahaji A."xsd:string
http://purl.uniprot.org/citations/21624897http://purl.uniprot.org/core/author"Ezquer I."xsd:string
http://purl.uniprot.org/citations/21624897http://purl.uniprot.org/core/author"Ovecka M."xsd:string
http://purl.uniprot.org/citations/21624897http://purl.uniprot.org/core/author"Sesma M.T."xsd:string
http://purl.uniprot.org/citations/21624897http://purl.uniprot.org/core/date"2011"xsd:gYear
http://purl.uniprot.org/citations/21624897http://purl.uniprot.org/core/name"Plant Cell Physiol"xsd:string
http://purl.uniprot.org/citations/21624897http://purl.uniprot.org/core/pages"1162-1176"xsd:string
http://purl.uniprot.org/citations/21624897http://purl.uniprot.org/core/title"Arabidopsis thaliana mutants lacking ADP-glucose pyrophosphorylase accumulate starch and wild-type ADP-glucose content: further evidence for the occurrence of important sources, other than ADP-glucose pyrophosphorylase, of ADP-glucose linked to leaf starch biosynthesis."xsd:string
http://purl.uniprot.org/citations/21624897http://purl.uniprot.org/core/volume"52"xsd:string
http://purl.uniprot.org/citations/21624897http://www.w3.org/2004/02/skos/core#exactMatchhttp://purl.uniprot.org/pubmed/21624897
http://purl.uniprot.org/citations/21624897http://xmlns.com/foaf/0.1/primaryTopicOfhttps://pubmed.ncbi.nlm.nih.gov/21624897
http://purl.uniprot.org/uniprot/#_A0A178UDC3-mappedCitation-21624897http://www.w3.org/1999/02/22-rdf-syntax-ns#objecthttp://purl.uniprot.org/citations/21624897
http://purl.uniprot.org/uniprot/#_P55228-mappedCitation-21624897http://www.w3.org/1999/02/22-rdf-syntax-ns#objecthttp://purl.uniprot.org/citations/21624897
http://purl.uniprot.org/uniprot/#_Q56ZU5-mappedCitation-21624897http://www.w3.org/1999/02/22-rdf-syntax-ns#objecthttp://purl.uniprot.org/citations/21624897
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