| http://purl.uniprot.org/citations/21625959 | http://www.w3.org/1999/02/22-rdf-syntax-ns#type | http://purl.uniprot.org/core/Journal_Citation |
| http://purl.uniprot.org/citations/21625959 | http://www.w3.org/2000/01/rdf-schema#comment | "HUVEC or mouse 3T3 cells infected with SV-40 generate within 3 to 5 days post-infection an ENOX2 species corresponding to the exon-4 minus splice variant of a tumor-associated NADH oxidase (ENOX2 or tNOX) expressed at the cancer cell surface. This study was to seek evidence for splicing factors that might direct formation of the exon 4 minus ENOX2 splice variant. To determine if silencing of ENOX2 exon 4 occurs because of motifs located in exon 4, transfections were performed on MCF-10A (mammary non-cancer), BT-20 (mammary cancer), and HeLa (cervical cancer) cells using a GFP minigene construct containing either a constitutively spliced exon (albumin exon 2) or the alternatively spliced ENOX2 exon 4 between the two GFP halves. Removal of exon 4 from the processed RNA of the GFP minigene construct occurred with HeLa and to a lesser extent with BT-20 but not in non-cancer MCF-10A cells. The Splicing Rainbow Program was used to identify all of the possible hnRNPs binding sites of exon 4 of ENOX2. There are 8 Exonic Splicing Silencers (ESSs) for hnRNP binding in the exon 4 sequences. Each of these sites were mutated by site-directed mutagenesis to test if any were responsible for the splicing skip. Results showed MutG75 ESS mutation changed the GFP expression which is a sign of splicing silence, while other mutations did not. As MutG75 changed the ESS binding site for hnRNP F, this result suggests that hnRNP F directs formation of the exon 4 minus variant of ENOX2."xsd:string |
| http://purl.uniprot.org/citations/21625959 | http://purl.org/dc/terms/identifier | "doi:10.1007/s11010-011-0875-5"xsd:string |
| http://purl.uniprot.org/citations/21625959 | http://purl.uniprot.org/core/author | "Tang X."xsd:string |
| http://purl.uniprot.org/citations/21625959 | http://purl.uniprot.org/core/author | "Morre D.J."xsd:string |
| http://purl.uniprot.org/citations/21625959 | http://purl.uniprot.org/core/author | "Morre D.M."xsd:string |
| http://purl.uniprot.org/citations/21625959 | http://purl.uniprot.org/core/author | "Kane V.D."xsd:string |
| http://purl.uniprot.org/citations/21625959 | http://purl.uniprot.org/core/date | "2011"xsd:gYear |
| http://purl.uniprot.org/citations/21625959 | http://purl.uniprot.org/core/name | "Mol Cell Biochem"xsd:string |
| http://purl.uniprot.org/citations/21625959 | http://purl.uniprot.org/core/pages | "55-63"xsd:string |
| http://purl.uniprot.org/citations/21625959 | http://purl.uniprot.org/core/title | "hnRNP F directs formation of an exon 4 minus variant of tumor-associated NADH oxidase (ENOX2)."xsd:string |
| http://purl.uniprot.org/citations/21625959 | http://purl.uniprot.org/core/volume | "357"xsd:string |
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