| http://purl.uniprot.org/citations/21668450 | http://www.w3.org/1999/02/22-rdf-syntax-ns#type | http://purl.uniprot.org/core/Journal_Citation |
| http://purl.uniprot.org/citations/21668450 | http://www.w3.org/1999/02/22-rdf-syntax-ns#type | http://purl.uniprot.org/core/Journal_Citation |
| http://purl.uniprot.org/citations/21668450 | http://www.w3.org/2000/01/rdf-schema#comment | "Abnormal regulation of brain glycogen metabolism is believed to underlie insulin-induced hypoglycaemia, which may be serious or fatal in diabetic patients on insulin therapy. A key regulator of glycogen levels is glycogen targeted protein phosphatase 1 (PP1), which dephosphorylates and activates glycogen synthase (GS) leading to an increase in glycogen synthesis. In this study, we show that the gene PPP1R3F expresses a glycogen-binding protein (R3F) of 82.8 kDa, present at the high levels in rodent brain. R3F binds to PP1 through a classical 'RVxF' binding motif and substitution of Phe39 for Ala in this motif abrogates PP1 binding. A hydrophobic domain at the carboxy-terminus of R3F has similarities to the putative membrane binding domain near the carboxy-terminus of striated muscle glycogen targeting subunit G(M)/R(GL), and R3F is shown to bind not only to glycogen but also to membranes. GS interacts with PP1-R3F and is hyperphosphorylated at glycogen synthase kinase-3 sites (Ser640 and Ser644) when bound to R3F(Phe39Ala). Deprivation of glucose or stimulation with adenosine or noradrenaline leads to an increased phosphorylation of PP1-R3F bound GS at Ser640 and Ser644 curtailing glycogen synthesis and facilitating glycogen degradation to provide glucose in astrocytoma cells. Adenosine stimulation also modulates phosphorylation of R3F at Ser14/Ser18."xsd:string |
| http://purl.uniprot.org/citations/21668450 | http://purl.org/dc/terms/identifier | "doi:10.1111/j.1471-4159.2011.07345.x"xsd:string |
| http://purl.uniprot.org/citations/21668450 | http://purl.org/dc/terms/identifier | "doi:10.1111/j.1471-4159.2011.07345.x"xsd:string |
| http://purl.uniprot.org/citations/21668450 | http://purl.uniprot.org/core/author | "Voss M."xsd:string |
| http://purl.uniprot.org/citations/21668450 | http://purl.uniprot.org/core/author | "Voss M."xsd:string |
| http://purl.uniprot.org/citations/21668450 | http://purl.uniprot.org/core/author | "Munro S."xsd:string |
| http://purl.uniprot.org/citations/21668450 | http://purl.uniprot.org/core/author | "Munro S."xsd:string |
| http://purl.uniprot.org/citations/21668450 | http://purl.uniprot.org/core/author | "Kelsall I.R."xsd:string |
| http://purl.uniprot.org/citations/21668450 | http://purl.uniprot.org/core/author | "Kelsall I.R."xsd:string |
| http://purl.uniprot.org/citations/21668450 | http://purl.uniprot.org/core/author | "Cohen P.T."xsd:string |
| http://purl.uniprot.org/citations/21668450 | http://purl.uniprot.org/core/author | "Cohen P.T."xsd:string |
| http://purl.uniprot.org/citations/21668450 | http://purl.uniprot.org/core/author | "Cuthbertson D.J."xsd:string |
| http://purl.uniprot.org/citations/21668450 | http://purl.uniprot.org/core/author | "Cuthbertson D.J."xsd:string |
| http://purl.uniprot.org/citations/21668450 | http://purl.uniprot.org/core/date | "2011"xsd:gYear |
| http://purl.uniprot.org/citations/21668450 | http://purl.uniprot.org/core/date | "2011"xsd:gYear |
| http://purl.uniprot.org/citations/21668450 | http://purl.uniprot.org/core/name | "J. Neurochem."xsd:string |
| http://purl.uniprot.org/citations/21668450 | http://purl.uniprot.org/core/name | "J. Neurochem."xsd:string |
| http://purl.uniprot.org/citations/21668450 | http://purl.uniprot.org/core/pages | "596-610"xsd:string |
| http://purl.uniprot.org/citations/21668450 | http://purl.uniprot.org/core/pages | "596-610"xsd:string |
| http://purl.uniprot.org/citations/21668450 | http://purl.uniprot.org/core/title | "R3F, a novel membrane-associated glycogen targeting subunit of protein phosphatase 1 regulates glycogen synthase in astrocytoma cells in response to glucose and extracellular signals."xsd:string |
| http://purl.uniprot.org/citations/21668450 | http://purl.uniprot.org/core/title | "R3F, a novel membrane-associated glycogen targeting subunit of protein phosphatase 1 regulates glycogen synthase in astrocytoma cells in response to glucose and extracellular signals."xsd:string |
| http://purl.uniprot.org/citations/21668450 | http://purl.uniprot.org/core/volume | "118"xsd:string |
| http://purl.uniprot.org/citations/21668450 | http://purl.uniprot.org/core/volume | "118"xsd:string |