| http://purl.uniprot.org/citations/21677144 | http://www.w3.org/1999/02/22-rdf-syntax-ns#type | http://purl.uniprot.org/core/Journal_Citation |
| http://purl.uniprot.org/citations/21677144 | http://www.w3.org/2000/01/rdf-schema#comment | "Galectins (Gal) are β-galactoside-binding proteins that function in epithelial development and homeostasis. An overlapping role for Gal-3 and Gal-7 in wound repair was reported in stratified epithelia. Although Gal-7 was thought absent in simple epithelia, it was reported in a proteomic analysis of cilia isolated from cultured human airway, and we recently identified Gal-7 transcripts in Madin-Darby canine kidney (MDCK) cells (Poland PA, Rondanino C, Kinlough CL, Heimburg-Molinaro J, Arthur CM, Stowell SR, Smith DF, Hughey RP. J Biol Chem 286: 6780-6790, 2011). We now report that Gal-7 is localized exclusively on the primary cilium of MDCK, LLC-PK(1) (pig kidney), and mpkCCD(c14) (mouse kidney) cells as well as on cilia in the rat renal proximal tubule. Gal-7 is also present on most cilia of multiciliated cells in human airway epithelia primary cultures. Interestingly, exogenous glutathione S-transferase (GST)-Gal-7 bound the MDCK apical plasma membrane as well as the cilium, while the lectin Ulex europeaus agglutinin, with glycan preferences similar to Gal-7, bound the basolateral plasma membrane as well as the cilium. In pull-down assays, β1-integrin isolated from either the basolateral or apical/cilia membranes of MDCK cells was similarly bound by GST-Gal-7. Selective localization of Gal-7 to cilia despite the presence of binding sites on all cell surfaces suggests that intracellular Gal-7 is specifically delivered to cilia rather than simply binding to surface glycoconjugates after generalized secretion. Moreover, depletion of Gal-7 using tetracycline-induced short-hairpin RNA in mpkCCD(c14) cells significantly reduced cilia length and slowed wound healing in a scratch assay. We conclude that Gal-7 is selectively targeted to cilia and plays a key role in surface stabilization of glycoconjugates responsible for integrating cilia function with epithelial repair."xsd:string |
| http://purl.uniprot.org/citations/21677144 | http://purl.org/dc/terms/identifier | "doi:10.1152/ajprenal.00134.2011"xsd:string |
| http://purl.uniprot.org/citations/21677144 | http://purl.uniprot.org/core/author | "Li H."xsd:string |
| http://purl.uniprot.org/citations/21677144 | http://purl.uniprot.org/core/author | "Hallows K.R."xsd:string |
| http://purl.uniprot.org/citations/21677144 | http://purl.uniprot.org/core/author | "Hughey R.P."xsd:string |
| http://purl.uniprot.org/citations/21677144 | http://purl.uniprot.org/core/author | "Kinlough C.L."xsd:string |
| http://purl.uniprot.org/citations/21677144 | http://purl.uniprot.org/core/author | "Poland P.A."xsd:string |
| http://purl.uniprot.org/citations/21677144 | http://purl.uniprot.org/core/author | "Myerburg M.M."xsd:string |
| http://purl.uniprot.org/citations/21677144 | http://purl.uniprot.org/core/author | "Weisz O.A."xsd:string |
| http://purl.uniprot.org/citations/21677144 | http://purl.uniprot.org/core/author | "Apodaca G."xsd:string |
| http://purl.uniprot.org/citations/21677144 | http://purl.uniprot.org/core/author | "Rbaibi Y."xsd:string |
| http://purl.uniprot.org/citations/21677144 | http://purl.uniprot.org/core/author | "Kashlan O.B."xsd:string |
| http://purl.uniprot.org/citations/21677144 | http://purl.uniprot.org/core/author | "Pastor-Soler N.M."xsd:string |
| http://purl.uniprot.org/citations/21677144 | http://purl.uniprot.org/core/author | "Rondanino C."xsd:string |
| http://purl.uniprot.org/citations/21677144 | http://purl.uniprot.org/core/author | "Al-bataineh M.M."xsd:string |
| http://purl.uniprot.org/citations/21677144 | http://purl.uniprot.org/core/date | "2011"xsd:gYear |
| http://purl.uniprot.org/citations/21677144 | http://purl.uniprot.org/core/name | "Am J Physiol Renal Physiol"xsd:string |
| http://purl.uniprot.org/citations/21677144 | http://purl.uniprot.org/core/pages | "F622-33"xsd:string |
| http://purl.uniprot.org/citations/21677144 | http://purl.uniprot.org/core/title | "Galectin-7 modulates the length of the primary cilia and wound repair in polarized kidney epithelial cells."xsd:string |
| http://purl.uniprot.org/citations/21677144 | http://purl.uniprot.org/core/volume | "301"xsd:string |
| http://purl.uniprot.org/citations/21677144 | http://www.w3.org/2004/02/skos/core#exactMatch | http://purl.uniprot.org/pubmed/21677144 |
| http://purl.uniprot.org/citations/21677144 | http://xmlns.com/foaf/0.1/primaryTopicOf | https://pubmed.ncbi.nlm.nih.gov/21677144 |
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| http://purl.uniprot.org/uniprot/#_P97590-mappedCitation-21677144 | http://www.w3.org/1999/02/22-rdf-syntax-ns#object | http://purl.uniprot.org/citations/21677144 |