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http://purl.uniprot.org/citations/21683597http://www.w3.org/1999/02/22-rdf-syntax-ns#typehttp://purl.uniprot.org/core/Journal_Citation
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Background

Signaling by receptor tyrosine kinase (RTK) pathways plays fundamental roles in processes of cell-fate determination, often through the induction of specific transcriptional responses. Yet it is not fully understood how continuous target gene expression, required for irreversible cell-fate specification, is preserved after RTK signaling has ended. Here we address this question using the Drosophila embryo, a model system that has been instrumental in elucidating the developmental functions of RTK signal transduction.

Results

The Groucho corepressor is phosphorylated and downregulated in response to RTK signaling. Here we show that RTK pathways use Groucho phosphorylation as a general mechanism for inducing expression of pathway target genes encoding cell-fate determinants as well as feedback antagonists, indicating that relief of Groucho-dependent repression is an integral element of RTK signaling networks. We further demonstrate that after mitogen-activated protein kinase (MAPK) has been deactivated, sustained phosphorylation of Groucho is essential for persistent RTK-induced target gene expression and cell-fate determination in several developmental contexts.

Conclusions

Phosphorylation of Groucho by MAPK plays a dual role in the regulation of RTK responses: (1) it mediates rapid feedback inhibition, and (2) it provides a stable memory mechanism of past MAPK activity. We propose that, in this manner, phosphorylation of Groucho enables transiently active RTK pathways to fix the spatiotemporal expression profiles of downstream targets over time."xsd:string
http://purl.uniprot.org/citations/21683597http://purl.org/dc/terms/identifier"doi:10.1016/j.cub.2011.05.043"xsd:string
http://purl.uniprot.org/citations/21683597http://purl.uniprot.org/core/author"Orian A."xsd:string
http://purl.uniprot.org/citations/21683597http://purl.uniprot.org/core/author"Hayouka Z."xsd:string
http://purl.uniprot.org/citations/21683597http://purl.uniprot.org/core/author"Cinnamon E."xsd:string
http://purl.uniprot.org/citations/21683597http://purl.uniprot.org/core/author"Helman A."xsd:string
http://purl.uniprot.org/citations/21683597http://purl.uniprot.org/core/author"Jimenez G."xsd:string
http://purl.uniprot.org/citations/21683597http://purl.uniprot.org/core/author"Paroush Z."xsd:string
http://purl.uniprot.org/citations/21683597http://purl.uniprot.org/core/author"Von Ohlen T."xsd:string
http://purl.uniprot.org/citations/21683597http://purl.uniprot.org/core/author"Mezuman S."xsd:string
http://purl.uniprot.org/citations/21683597http://purl.uniprot.org/core/date"2011"xsd:gYear
http://purl.uniprot.org/citations/21683597http://purl.uniprot.org/core/name"Curr Biol"xsd:string
http://purl.uniprot.org/citations/21683597http://purl.uniprot.org/core/pages"1102-1110"xsd:string
http://purl.uniprot.org/citations/21683597http://purl.uniprot.org/core/title"Phosphorylation of Groucho mediates RTK feedback inhibition and prolonged pathway target gene expression."xsd:string
http://purl.uniprot.org/citations/21683597http://purl.uniprot.org/core/volume"21"xsd:string
http://purl.uniprot.org/citations/21683597http://www.w3.org/2004/02/skos/core#exactMatchhttp://purl.uniprot.org/pubmed/21683597
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http://purl.uniprot.org/uniprot/P16371#attribution-CB64403EB7FE15E442877DFB8494836Ehttp://purl.uniprot.org/core/sourcehttp://purl.uniprot.org/citations/21683597
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