| http://purl.uniprot.org/citations/21699496 | http://www.w3.org/1999/02/22-rdf-syntax-ns#type | http://purl.uniprot.org/core/Journal_Citation |
| http://purl.uniprot.org/citations/21699496 | http://www.w3.org/1999/02/22-rdf-syntax-ns#type | http://purl.uniprot.org/core/Journal_Citation |
| http://purl.uniprot.org/citations/21699496 | http://www.w3.org/2000/01/rdf-schema#comment | "CRISPR (clustered regularly interspaced short palindromic repeat)/Cas (CRISPR-associated) is a nucleic acid processing system in bacteria and archaea that interacts with mobile genetic elements. CRISPR DNA and RNA sequences are processed by Cas proteins: in Escherichia coli K-12, one CRISPR locus links to eight cas genes (cas1, 2, 3 and casABCDE), whose protein products promote protection against phage. In the present paper, we report that purified E. coli Cas3 catalyses ATP-independent annealing of RNA with DNA forming R-loops, hybrids of RNA base-paired into duplex DNA. ATP abolishes Cas3 R-loop formation and instead powers Cas3 helicase unwinding of the invading RNA strand of a model R-loop substrate. R-loop formation by Cas3 requires magnesium as a co-factor and is inactivated by mutagenesis of a conserved amino acid motif. Cells expressing the mutant Cas3 protein are more sensitive to plaque formation by the phage λvir. A complex of CasABCDE ('Cascade') also promotes R-loop formation and we discuss possible overlapping roles of Cas3 and Cascade in E. coli, and the apparently antagonistic roles of Cas3 catalysing RNA-DNA annealing and ATP-dependent helicase unwinding."xsd:string |
| http://purl.uniprot.org/citations/21699496 | http://purl.org/dc/terms/identifier | "doi:10.1042/bj20110901"xsd:string |
| http://purl.uniprot.org/citations/21699496 | http://purl.org/dc/terms/identifier | "doi:10.1042/bj20110901"xsd:string |
| http://purl.uniprot.org/citations/21699496 | http://purl.uniprot.org/core/author | "Bolt E.L."xsd:string |
| http://purl.uniprot.org/citations/21699496 | http://purl.uniprot.org/core/author | "Bolt E.L."xsd:string |
| http://purl.uniprot.org/citations/21699496 | http://purl.uniprot.org/core/author | "Delmas S."xsd:string |
| http://purl.uniprot.org/citations/21699496 | http://purl.uniprot.org/core/author | "Delmas S."xsd:string |
| http://purl.uniprot.org/citations/21699496 | http://purl.uniprot.org/core/author | "Howard J.A."xsd:string |
| http://purl.uniprot.org/citations/21699496 | http://purl.uniprot.org/core/author | "Howard J.A."xsd:string |
| http://purl.uniprot.org/citations/21699496 | http://purl.uniprot.org/core/author | "Ivancic-Bace I."xsd:string |
| http://purl.uniprot.org/citations/21699496 | http://purl.uniprot.org/core/author | "Ivancic-Bace I."xsd:string |
| http://purl.uniprot.org/citations/21699496 | http://purl.uniprot.org/core/date | "2011"xsd:gYear |
| http://purl.uniprot.org/citations/21699496 | http://purl.uniprot.org/core/date | "2011"xsd:gYear |
| http://purl.uniprot.org/citations/21699496 | http://purl.uniprot.org/core/name | "Biochem. J."xsd:string |
| http://purl.uniprot.org/citations/21699496 | http://purl.uniprot.org/core/name | "Biochem. J."xsd:string |
| http://purl.uniprot.org/citations/21699496 | http://purl.uniprot.org/core/pages | "85-95"xsd:string |
| http://purl.uniprot.org/citations/21699496 | http://purl.uniprot.org/core/pages | "85-95"xsd:string |
| http://purl.uniprot.org/citations/21699496 | http://purl.uniprot.org/core/title | "Helicase dissociation and annealing of RNA-DNA hybrids by Escherichia coli Cas3 protein."xsd:string |
| http://purl.uniprot.org/citations/21699496 | http://purl.uniprot.org/core/title | "Helicase dissociation and annealing of RNA-DNA hybrids by Escherichia coli Cas3 protein."xsd:string |
| http://purl.uniprot.org/citations/21699496 | http://purl.uniprot.org/core/volume | "439"xsd:string |
| http://purl.uniprot.org/citations/21699496 | http://purl.uniprot.org/core/volume | "439"xsd:string |
| http://purl.uniprot.org/citations/21699496 | http://www.w3.org/2004/02/skos/core#exactMatch | http://purl.uniprot.org/pubmed/21699496 |
| http://purl.uniprot.org/citations/21699496 | http://www.w3.org/2004/02/skos/core#exactMatch | http://purl.uniprot.org/pubmed/21699496 |