| http://purl.uniprot.org/citations/21705623 | http://www.w3.org/1999/02/22-rdf-syntax-ns#type | http://purl.uniprot.org/core/Journal_Citation |
| http://purl.uniprot.org/citations/21705623 | http://www.w3.org/2000/01/rdf-schema#comment | "MHC class I-restricted CD8(+) T cells play an important role in protective immunity against mycobacteria. Previously, we showed that p113-121, derived from Mycobacterium leprae protein ML1419c, induced significant IFN-γ production by CD8(+) T cells in 90% of paucibacillary leprosy patients and in 80% of multibacillary patients' contacts, demonstrating induction of M. leprae-specific CD8(+) T cell immunity. In this work, we studied the in vivo role and functional profile of ML1419c p113-121-induced T cells in HLA-A*0201 transgenic mice. Immunization with 9mer or 30mer covering the p113-121 sequence combined with TLR9 agonist CpG induced HLA-A*0201-restricted, M. leprae-specific CD8(+) T cells as visualized by p113-121/HLA-A*0201 tetramers. Most CD8(+) T cells produced IFN-γ, but distinct IFN-γ(+)/TNF-α(+) populations were detected simultaneously with significant secretion of CXCL10/IFN-γ-induced protein 10, CXCL9/MIG, and VEGF. Strikingly, peptide immunization also induced high ML1419c-specific IgG levels, strongly suggesting that peptide-specific CD8(+) T cells provide help to B cells in vivo, as CD4(+) T cells were undetectable. An additional important characteristic of p113-121-specific CD8(+) T cells was their capacity for in vivo killing of p113-121-labeled, HLA-A*0201(+) splenocytes. The cytotoxic function of p113-121/HLA-A*0201-specific CD8(+) T cells extended into direct killing of splenocytes infected with live Mycobacterium smegmatis expressing ML1419c: both 9mer and 30mer induced CD8(+) T cells that reduced the number of ML1419c-expressing mycobacteria by 95%, whereas no reduction occurred using wild-type M. smegmatis. These data, combined with previous observations in Brazilian cohorts, show that ML1419c p113-121 induces potent CD8(+) T cells that provide protective immunity against M. leprae and B cell help for induction of specific IgG, suggesting its potential use in diagnostics and as a subunit (vaccine) for M. leprae infection."xsd:string |
| http://purl.uniprot.org/citations/21705623 | http://purl.org/dc/terms/identifier | "doi:10.4049/jimmunol.1100980"xsd:string |
| http://purl.uniprot.org/citations/21705623 | http://purl.uniprot.org/core/author | "Kim H.J."xsd:string |
| http://purl.uniprot.org/citations/21705623 | http://purl.uniprot.org/core/author | "Spencer J.S."xsd:string |
| http://purl.uniprot.org/citations/21705623 | http://purl.uniprot.org/core/author | "Franken K.L."xsd:string |
| http://purl.uniprot.org/citations/21705623 | http://purl.uniprot.org/core/author | "Ottenhoff T.H."xsd:string |
| http://purl.uniprot.org/citations/21705623 | http://purl.uniprot.org/core/author | "Wilson L."xsd:string |
| http://purl.uniprot.org/citations/21705623 | http://purl.uniprot.org/core/author | "Geluk A."xsd:string |
| http://purl.uniprot.org/citations/21705623 | http://purl.uniprot.org/core/author | "Pereira G.M."xsd:string |
| http://purl.uniprot.org/citations/21705623 | http://purl.uniprot.org/core/author | "van den Eeden S.J."xsd:string |
| http://purl.uniprot.org/citations/21705623 | http://purl.uniprot.org/core/author | "Pessolani M.C."xsd:string |
| http://purl.uniprot.org/citations/21705623 | http://purl.uniprot.org/core/author | "Dijkman K."xsd:string |
| http://purl.uniprot.org/citations/21705623 | http://purl.uniprot.org/core/date | "2011"xsd:gYear |
| http://purl.uniprot.org/citations/21705623 | http://purl.uniprot.org/core/name | "J Immunol"xsd:string |
| http://purl.uniprot.org/citations/21705623 | http://purl.uniprot.org/core/pages | "1393-1402"xsd:string |
| http://purl.uniprot.org/citations/21705623 | http://purl.uniprot.org/core/title | "ML1419c peptide immunization induces Mycobacterium leprae-specific HLA-A*0201-restricted CTL in vivo with potential to kill live mycobacteria."xsd:string |
| http://purl.uniprot.org/citations/21705623 | http://purl.uniprot.org/core/volume | "187"xsd:string |
| http://purl.uniprot.org/citations/21705623 | http://www.w3.org/2004/02/skos/core#exactMatch | http://purl.uniprot.org/pubmed/21705623 |
| http://purl.uniprot.org/citations/21705623 | http://xmlns.com/foaf/0.1/primaryTopicOf | https://pubmed.ncbi.nlm.nih.gov/21705623 |
| http://purl.uniprot.org/uniprot/#_A0A0A7C543-mappedCitation-21705623 | http://www.w3.org/1999/02/22-rdf-syntax-ns#object | http://purl.uniprot.org/citations/21705623 |
| http://purl.uniprot.org/uniprot/#_A0A0A7C548-mappedCitation-21705623 | http://www.w3.org/1999/02/22-rdf-syntax-ns#object | http://purl.uniprot.org/citations/21705623 |
| http://purl.uniprot.org/uniprot/#_A0A0A7C551-mappedCitation-21705623 | http://www.w3.org/1999/02/22-rdf-syntax-ns#object | http://purl.uniprot.org/citations/21705623 |
| http://purl.uniprot.org/uniprot/#_A0A0G2R0N3-mappedCitation-21705623 | http://www.w3.org/1999/02/22-rdf-syntax-ns#object | http://purl.uniprot.org/citations/21705623 |
| http://purl.uniprot.org/uniprot/#_A0A0G2R0N4-mappedCitation-21705623 | http://www.w3.org/1999/02/22-rdf-syntax-ns#object | http://purl.uniprot.org/citations/21705623 |