http://purl.uniprot.org/citations/21723285 | http://www.w3.org/1999/02/22-rdf-syntax-ns#type | http://purl.uniprot.org/core/Journal_Citation |
http://purl.uniprot.org/citations/21723285 | http://www.w3.org/2000/01/rdf-schema#comment | "Cytoplasmic dynein is a microtubule (MT) motor protein comprising two classes: dynein-1 and dynein-2. We purified recombinant human dynein-1 and dynein-2 from HEK-293 cells by expressing the streptavidin-binding peptide-tagged human cytoplasmic dynein-1 and dynein-2 heavy chains (HCs), respectively. Electron microscopy of the purified molecules revealed a two-headed structure composed of characteristic dynein motor domains. In an in vitro MT gliding assay, both dynein-1 and dynein-2 showed minus-end-directed motor activities. This is the first demonstration of dynein-2 motor activity, which supports the retrograde intraflagellar transport role of dynein-2. Our expression system of dynein HCs provides a useful means to investigate dynein functions."xsd:string |
http://purl.uniprot.org/citations/21723285 | http://purl.org/dc/terms/identifier | "doi:10.1016/j.febslet.2011.06.026"xsd:string |
http://purl.uniprot.org/citations/21723285 | http://purl.uniprot.org/core/author | "Ichikawa M."xsd:string |
http://purl.uniprot.org/citations/21723285 | http://purl.uniprot.org/core/author | "Watanabe Y."xsd:string |
http://purl.uniprot.org/citations/21723285 | http://purl.uniprot.org/core/author | "Murayama T."xsd:string |
http://purl.uniprot.org/citations/21723285 | http://purl.uniprot.org/core/author | "Toyoshima Y.Y."xsd:string |
http://purl.uniprot.org/citations/21723285 | http://purl.uniprot.org/core/date | "2011"xsd:gYear |
http://purl.uniprot.org/citations/21723285 | http://purl.uniprot.org/core/name | "FEBS Lett"xsd:string |
http://purl.uniprot.org/citations/21723285 | http://purl.uniprot.org/core/pages | "2419-2423"xsd:string |
http://purl.uniprot.org/citations/21723285 | http://purl.uniprot.org/core/title | "Recombinant human cytoplasmic dynein heavy chain 1 and 2: observation of dynein-2 motor activity in vitro."xsd:string |
http://purl.uniprot.org/citations/21723285 | http://purl.uniprot.org/core/volume | "585"xsd:string |
http://purl.uniprot.org/citations/21723285 | http://www.w3.org/2004/02/skos/core#exactMatch | http://purl.uniprot.org/pubmed/21723285 |
http://purl.uniprot.org/citations/21723285 | http://xmlns.com/foaf/0.1/primaryTopicOf | https://pubmed.ncbi.nlm.nih.gov/21723285 |
http://purl.uniprot.org/uniprot/Q8NCM8#attribution-26F8DE0754CBF4F9E2B4E9EE1A8A35FA | http://purl.uniprot.org/core/source | http://purl.uniprot.org/citations/21723285 |
http://purl.uniprot.org/uniprot/Q14204#attribution-26F8DE0754CBF4F9E2B4E9EE1A8A35FA | http://purl.uniprot.org/core/source | http://purl.uniprot.org/citations/21723285 |
http://purl.uniprot.org/uniprot/#_Q14204-mappedCitation-21723285 | http://www.w3.org/1999/02/22-rdf-syntax-ns#object | http://purl.uniprot.org/citations/21723285 |
http://purl.uniprot.org/uniprot/#_B4DSR6-mappedCitation-21723285 | http://www.w3.org/1999/02/22-rdf-syntax-ns#object | http://purl.uniprot.org/citations/21723285 |
http://purl.uniprot.org/uniprot/#_Q6P2H7-mappedCitation-21723285 | http://www.w3.org/1999/02/22-rdf-syntax-ns#object | http://purl.uniprot.org/citations/21723285 |
http://purl.uniprot.org/uniprot/#_Q92862-mappedCitation-21723285 | http://www.w3.org/1999/02/22-rdf-syntax-ns#object | http://purl.uniprot.org/citations/21723285 |
http://purl.uniprot.org/uniprot/#_Q9NRC3-mappedCitation-21723285 | http://www.w3.org/1999/02/22-rdf-syntax-ns#object | http://purl.uniprot.org/citations/21723285 |
http://purl.uniprot.org/uniprot/#_Q8NCM8-mappedCitation-21723285 | http://www.w3.org/1999/02/22-rdf-syntax-ns#object | http://purl.uniprot.org/citations/21723285 |
http://purl.uniprot.org/uniprot/Q92862 | http://purl.uniprot.org/core/mappedCitation | http://purl.uniprot.org/citations/21723285 |
http://purl.uniprot.org/uniprot/Q8NCM8 | http://purl.uniprot.org/core/mappedCitation | http://purl.uniprot.org/citations/21723285 |
http://purl.uniprot.org/uniprot/B4DSR6 | http://purl.uniprot.org/core/mappedCitation | http://purl.uniprot.org/citations/21723285 |