| http://purl.uniprot.org/citations/2173696 | http://www.w3.org/1999/02/22-rdf-syntax-ns#type | http://purl.uniprot.org/core/Journal_Citation |
| http://purl.uniprot.org/citations/2173696 | http://www.w3.org/1999/02/22-rdf-syntax-ns#type | http://purl.uniprot.org/core/Journal_Citation |
| http://purl.uniprot.org/citations/2173696 | http://www.w3.org/2000/01/rdf-schema#comment | "Two distinct tumor necrosis factor (TNF) receptors of 55- and 75-kDa apparent molecular masses previously identified on the cell surface by monoclonal antibodies have been solubilized with Triton X-100 from HL60 cells. A filter-based dot blot assay was developed to monitor specific 125I-TNF alpha binding during fractionation of the cell extract. By a combination of immuno- and ligand affinity chromatography and reverse phase high performance liquid chromatography both receptor proteins were purified to apparent homogeneity. Analysis by sodium dodecyl sulfate-polyacrylamide gel electrophoresis showed two bands at 55 and 51 kDa for the 55-kDa TNF receptor and a major 75-kDa and a minor 65-kDa band for the 75-kDa TNF receptor. All these bands specifically bound TNF alpha and TNF beta in ligand blot experiments. The exclusive specificity of monoclonal antibodies of the utr series for the 75.65-kDa bands and of the htr series for the 55.51-kDa bands was demonstrated with the purified antigens on Western blots. Both TNF receptor types were found to contain N-linked carbohydrates. N-terminal amino acid sequence analysis of the 55- and 51-kDa bands of the 55-kDa TNF receptor revealed identical sequences suggesting a possible truncation at the C-terminal end. Two different N-terminal sequences were determined for the 65-kDa band. One corresponded to the published sequence of ubiquitin; the other was therefore assumed to be a unique sequence of the 75-kDa TNF receptor. Additional internal sequences of this receptor were determined after proteolytic cleavage."xsd:string |
| http://purl.uniprot.org/citations/2173696 | http://purl.org/dc/terms/identifier | "doi:10.1016/s0021-9258(17)30479-9"xsd:string |
| http://purl.uniprot.org/citations/2173696 | http://purl.org/dc/terms/identifier | "doi:10.1016/s0021-9258(17)30479-9"xsd:string |
| http://purl.uniprot.org/citations/2173696 | http://purl.uniprot.org/core/author | "Lahm H.-W."xsd:string |
| http://purl.uniprot.org/citations/2173696 | http://purl.uniprot.org/core/author | "Lahm H.-W."xsd:string |
| http://purl.uniprot.org/citations/2173696 | http://purl.uniprot.org/core/author | "Loetscher H."xsd:string |
| http://purl.uniprot.org/citations/2173696 | http://purl.uniprot.org/core/author | "Loetscher H."xsd:string |
| http://purl.uniprot.org/citations/2173696 | http://purl.uniprot.org/core/author | "Pan Y.-C.E."xsd:string |
| http://purl.uniprot.org/citations/2173696 | http://purl.uniprot.org/core/author | "Pan Y.-C.E."xsd:string |
| http://purl.uniprot.org/citations/2173696 | http://purl.uniprot.org/core/author | "Lesslauer W."xsd:string |
| http://purl.uniprot.org/citations/2173696 | http://purl.uniprot.org/core/author | "Lesslauer W."xsd:string |
| http://purl.uniprot.org/citations/2173696 | http://purl.uniprot.org/core/author | "Brockhaus M."xsd:string |
| http://purl.uniprot.org/citations/2173696 | http://purl.uniprot.org/core/author | "Brockhaus M."xsd:string |
| http://purl.uniprot.org/citations/2173696 | http://purl.uniprot.org/core/author | "Schlaeger E.J."xsd:string |
| http://purl.uniprot.org/citations/2173696 | http://purl.uniprot.org/core/author | "Schlaeger E.J."xsd:string |
| http://purl.uniprot.org/citations/2173696 | http://purl.uniprot.org/core/date | "1990"xsd:gYear |
| http://purl.uniprot.org/citations/2173696 | http://purl.uniprot.org/core/date | "1990"xsd:gYear |
| http://purl.uniprot.org/citations/2173696 | http://purl.uniprot.org/core/name | "J. Biol. Chem."xsd:string |
| http://purl.uniprot.org/citations/2173696 | http://purl.uniprot.org/core/name | "J. Biol. Chem."xsd:string |
| http://purl.uniprot.org/citations/2173696 | http://purl.uniprot.org/core/pages | "20131-20138"xsd:string |
| http://purl.uniprot.org/citations/2173696 | http://purl.uniprot.org/core/pages | "20131-20138"xsd:string |
| http://purl.uniprot.org/citations/2173696 | http://purl.uniprot.org/core/title | "Purification and partial amino acid sequence analysis of two distinct tumor necrosis factor receptors from HL60 cells."xsd:string |
| http://purl.uniprot.org/citations/2173696 | http://purl.uniprot.org/core/title | "Purification and partial amino acid sequence analysis of two distinct tumor necrosis factor receptors from HL60 cells."xsd:string |