| http://purl.uniprot.org/citations/21738689 | http://www.w3.org/1999/02/22-rdf-syntax-ns#type | http://purl.uniprot.org/core/Journal_Citation |
| http://purl.uniprot.org/citations/21738689 | http://www.w3.org/1999/02/22-rdf-syntax-ns#type | http://purl.uniprot.org/core/Journal_Citation |
| http://purl.uniprot.org/citations/21738689 | http://www.w3.org/2000/01/rdf-schema#comment | "Transcriptional regulation is an important mechanism underlying gene expression and has played a crucial role in evolution. The number, position and interactions between cis-elements and transcription factors (TFs) determine the expression pattern of a gene. To identify functionally relevant cis-elements in gene promoters, a phylogenetic shadowing approach with a lipase gene (LIP1) was used. As a proof of concept, in silico analyses of several Brassicaceae LIP1 promoters identified a highly conserved sequence (LIP1 element) that is sufficient to drive strong expression of a reporter gene in planta. A collection of ca. 1,200 Arabidopsis thaliana TF open reading frames (ORFs) was arrayed in a 96-well format (RR library) and a convenient mating based yeast one hybrid (Y1H) screening procedure was established. We constructed an episomal plasmid (pTUY1H) to clone the LIP1 element and used it as bait for Y1H screenings. A novel interaction with an HD-ZIP (AtML1) TF was identified and abolished by a 2 bp mutation in the LIP1 element. A role of this interaction in transcriptional regulation was confirmed in planta. In addition, we validated our strategy by reproducing the previously reported interaction between a MYB-CC (PHR1) TF, a central regulator of phosphate starvation responses, with a conserved promoter fragment (IPS1 element) containing its cognate binding sequence. Finally, we established that the LIP1 and IPS1 elements were differentially bound by HD-ZIP and MYB-CC family members in agreement with their genetic redundancy in planta. In conclusion, combining in silico analyses of orthologous gene promoters with Y1H screening of the RR library represents a powerful approach to decipher cis- and trans-regulatory codes."xsd:string |
| http://purl.uniprot.org/citations/21738689 | http://purl.org/dc/terms/identifier | "doi:10.1371/journal.pone.0021524"xsd:string |
| http://purl.uniprot.org/citations/21738689 | http://purl.org/dc/terms/identifier | "doi:10.1371/journal.pone.0021524"xsd:string |
| http://purl.uniprot.org/citations/21738689 | http://purl.uniprot.org/core/author | "Lecharny A."xsd:string |
| http://purl.uniprot.org/citations/21738689 | http://purl.uniprot.org/core/author | "Lecharny A."xsd:string |
| http://purl.uniprot.org/citations/21738689 | http://purl.uniprot.org/core/author | "Paz-Ares J."xsd:string |
| http://purl.uniprot.org/citations/21738689 | http://purl.uniprot.org/core/author | "Paz-Ares J."xsd:string |
| http://purl.uniprot.org/citations/21738689 | http://purl.uniprot.org/core/author | "Carbonero P."xsd:string |
| http://purl.uniprot.org/citations/21738689 | http://purl.uniprot.org/core/author | "Carbonero P."xsd:string |
| http://purl.uniprot.org/citations/21738689 | http://purl.uniprot.org/core/author | "Onate-Sanchez L."xsd:string |
| http://purl.uniprot.org/citations/21738689 | http://purl.uniprot.org/core/author | "Onate-Sanchez L."xsd:string |
| http://purl.uniprot.org/citations/21738689 | http://purl.uniprot.org/core/author | "Coupland G."xsd:string |
| http://purl.uniprot.org/citations/21738689 | http://purl.uniprot.org/core/author | "Coupland G."xsd:string |
| http://purl.uniprot.org/citations/21738689 | http://purl.uniprot.org/core/author | "Turck F."xsd:string |
| http://purl.uniprot.org/citations/21738689 | http://purl.uniprot.org/core/author | "Turck F."xsd:string |
| http://purl.uniprot.org/citations/21738689 | http://purl.uniprot.org/core/author | "Castrillo G."xsd:string |
| http://purl.uniprot.org/citations/21738689 | http://purl.uniprot.org/core/author | "Castrillo G."xsd:string |
| http://purl.uniprot.org/citations/21738689 | http://purl.uniprot.org/core/author | "Leveugle M."xsd:string |
| http://purl.uniprot.org/citations/21738689 | http://purl.uniprot.org/core/author | "Leveugle M."xsd:string |
| http://purl.uniprot.org/citations/21738689 | http://purl.uniprot.org/core/date | "2011"xsd:gYear |
| http://purl.uniprot.org/citations/21738689 | http://purl.uniprot.org/core/date | "2011"xsd:gYear |
| http://purl.uniprot.org/citations/21738689 | http://purl.uniprot.org/core/name | "PLoS ONE"xsd:string |
| http://purl.uniprot.org/citations/21738689 | http://purl.uniprot.org/core/name | "PLoS ONE"xsd:string |