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http://purl.uniprot.org/citations/21739422http://www.w3.org/1999/02/22-rdf-syntax-ns#typehttp://purl.uniprot.org/core/Journal_Citation
http://purl.uniprot.org/citations/21739422http://www.w3.org/2000/01/rdf-schema#comment"

Objective

To investigate P- and E-selectin ligand coexpression with chemokine receptors (CKRs) on T cells in the synovial fluid (SF) and blood of children with juvenile idiopathic arthritis (JIA).

Methods

Sixteen patients with polyarticular or persistent oligoarticular JIA (ages 5.3-15.1 years) were studied. SF and venous blood were collected, and immunostaining for the expression of CCR4, CCR5, CXCR3, and P- or E-selectin ligands was performed.

Results

Compared to blood, SF was greatly enriched for CD4+ T cells bearing CCR5, CCR4, CXCR3, and both P- and E-selectin ligand. Twenty-five percent of the CD4+ T cells in SF expressed both CCR5 and CCR4, some also coexpressing CXCR3. Such cells were rare in blood. Half of the few CCR5+ T cells in blood coexpressed P- or E-selectin ligand, a phenotype that was enriched up to 50-fold in SF. A minority of CCR4+ and CXCR3+ cells in blood (∼25%) coexpressed selectin ligand; these were enriched 4-8-fold in SF. Most CCR4-expressing CD4+ T cells expressed both E-selectin ligand and cutaneous lymphocyte antigen.

Conclusion

CCR4-, CCR5-, CXCR3-, and selectin ligand-expressing CD4+ T cells preferentially accumulate in the joints of children with JIA. The marked enrichment of CCR5+ T cells coexpressing P-selectin and/or E-selectin ligand in CD4+ SF T cells suggests that the few such cells in blood selectively migrate to inflamed joints via endothelial P- and E-selectin- and CCR5-activating chemokines. The predominance of CCR4-expressing CD4+ T cells coexpressing E-selectin ligand suggests that such cells migrate not only to areas of cutaneous inflammation, as previously reported, but also to the joints in JIA. Combined targeting of CCR5- and E-selectin-dependent mechanisms may be a relevant treatment strategy."xsd:string
http://purl.uniprot.org/citations/21739422http://purl.org/dc/terms/identifier"doi:10.1002/art.30521"xsd:string
http://purl.uniprot.org/citations/21739422http://purl.uniprot.org/core/author"Ramsey S."xsd:string
http://purl.uniprot.org/citations/21739422http://purl.uniprot.org/core/author"Lang B."xsd:string
http://purl.uniprot.org/citations/21739422http://purl.uniprot.org/core/author"Issekutz A.C."xsd:string
http://purl.uniprot.org/citations/21739422http://purl.uniprot.org/core/author"Quinn P.J."xsd:string
http://purl.uniprot.org/citations/21739422http://purl.uniprot.org/core/author"Issekutz T.B."xsd:string
http://purl.uniprot.org/citations/21739422http://purl.uniprot.org/core/author"Huber A.M."xsd:string
http://purl.uniprot.org/citations/21739422http://purl.uniprot.org/core/author"Karkada M."xsd:string
http://purl.uniprot.org/citations/21739422http://purl.uniprot.org/core/author"Rowter D."xsd:string
http://purl.uniprot.org/citations/21739422http://purl.uniprot.org/core/date"2011"xsd:gYear
http://purl.uniprot.org/citations/21739422http://purl.uniprot.org/core/name"Arthritis Rheum"xsd:string
http://purl.uniprot.org/citations/21739422http://purl.uniprot.org/core/pages"3467-3476"xsd:string
http://purl.uniprot.org/citations/21739422http://purl.uniprot.org/core/title"Coexpression of chemokine receptors CCR5, CXCR3, and CCR4 and ligands for P- and E-selectin on T lymphocytes of patients with juvenile idiopathic arthritis."xsd:string
http://purl.uniprot.org/citations/21739422http://purl.uniprot.org/core/volume"63"xsd:string
http://purl.uniprot.org/citations/21739422http://www.w3.org/2004/02/skos/core#exactMatchhttp://purl.uniprot.org/pubmed/21739422
http://purl.uniprot.org/citations/21739422http://xmlns.com/foaf/0.1/primaryTopicOfhttps://pubmed.ncbi.nlm.nih.gov/21739422
http://purl.uniprot.org/uniprot/#_A0A089G6S6-mappedCitation-21739422http://www.w3.org/1999/02/22-rdf-syntax-ns#objecthttp://purl.uniprot.org/citations/21739422
http://purl.uniprot.org/uniprot/#_A0A089G6S9-mappedCitation-21739422http://www.w3.org/1999/02/22-rdf-syntax-ns#objecthttp://purl.uniprot.org/citations/21739422
http://purl.uniprot.org/uniprot/#_A0A089G7F7-mappedCitation-21739422http://www.w3.org/1999/02/22-rdf-syntax-ns#objecthttp://purl.uniprot.org/citations/21739422
http://purl.uniprot.org/uniprot/#_A0A089G7G0-mappedCitation-21739422http://www.w3.org/1999/02/22-rdf-syntax-ns#objecthttp://purl.uniprot.org/citations/21739422
http://purl.uniprot.org/uniprot/#_A0A089G7G7-mappedCitation-21739422http://www.w3.org/1999/02/22-rdf-syntax-ns#objecthttp://purl.uniprot.org/citations/21739422
http://purl.uniprot.org/uniprot/#_A0A089G7G9-mappedCitation-21739422http://www.w3.org/1999/02/22-rdf-syntax-ns#objecthttp://purl.uniprot.org/citations/21739422
http://purl.uniprot.org/uniprot/#_A0A089G3J8-mappedCitation-21739422http://www.w3.org/1999/02/22-rdf-syntax-ns#objecthttp://purl.uniprot.org/citations/21739422
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