| http://purl.uniprot.org/citations/21751213 | http://www.w3.org/1999/02/22-rdf-syntax-ns#type | http://purl.uniprot.org/core/Journal_Citation |
| http://purl.uniprot.org/citations/21751213 | http://www.w3.org/2000/01/rdf-schema#comment | "Ubiquitin C-terminal hydrolases (UCHs) comprise a family of deubiquitinating enzymes that play a role in the removal of multi-ubiquitin chains from proteins that are posttranslationally modified by ubiquitination to be targeted for proteolysis by the 26S proteasome. The UCH-enzymes also generate free monomeric ubiquitin from precursor multi-ubiquitin chains and, in some instances, may rescue ubiquitinated proteins from degradation. This study examined the roles of two oocyte-expressed UCHs, UCHL1, and UCHL3 in murine and rhesus monkey oocyte maturation. The Uchl1 and Uchl3 mRNAs were highly expressed in GV and MII oocytes, and were associated with the oocyte cortex (UCHL1) and meiotic spindle (UCHL3). Microinjection of the UCH-family enzyme inhibitor, ubiquitin-aldehyde (UBAL) to GV oocytes prevented oocyte meiotic progression beyond metaphase I in a majority of treated oocytes and caused spindle and first polar body anomalies. Injection of antibodies against UCHL3 disrupted oocyte maturation and caused meiotic anomalies, including abnormally long meiotic spindles. A selective, cell permeant inhibitor of UCHL3, 4, 5, 6, 7-tetrachloroidan-1, 3-dione also caused meiotic defects and chromosome misalignment. Cortical granule localization in the oocyte cortex was disrupted by UBAL injected after oocyte maturation. We conclude that the activity of oocyte UCHs contributes to oocyte maturation by regulating the oocyte cortex and meiotic spindle."xsd:string |
| http://purl.uniprot.org/citations/21751213 | http://purl.org/dc/terms/identifier | "doi:10.1002/jcp.22931"xsd:string |
| http://purl.uniprot.org/citations/21751213 | http://purl.uniprot.org/core/author | "Sutovsky P."xsd:string |
| http://purl.uniprot.org/citations/21751213 | http://purl.uniprot.org/core/author | "Latham K.E."xsd:string |
| http://purl.uniprot.org/citations/21751213 | http://purl.uniprot.org/core/author | "Sutovsky M."xsd:string |
| http://purl.uniprot.org/citations/21751213 | http://purl.uniprot.org/core/author | "Vandevoort C.A."xsd:string |
| http://purl.uniprot.org/citations/21751213 | http://purl.uniprot.org/core/author | "Mtango N.R."xsd:string |
| http://purl.uniprot.org/citations/21751213 | http://purl.uniprot.org/core/date | "2012"xsd:gYear |
| http://purl.uniprot.org/citations/21751213 | http://purl.uniprot.org/core/name | "J Cell Physiol"xsd:string |
| http://purl.uniprot.org/citations/21751213 | http://purl.uniprot.org/core/pages | "2022-2029"xsd:string |
| http://purl.uniprot.org/citations/21751213 | http://purl.uniprot.org/core/title | "Essential role of ubiquitin C-terminal hydrolases UCHL1 and UCHL3 in mammalian oocyte maturation."xsd:string |
| http://purl.uniprot.org/citations/21751213 | http://purl.uniprot.org/core/volume | "227"xsd:string |
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