| http://purl.uniprot.org/citations/21768390 | http://www.w3.org/1999/02/22-rdf-syntax-ns#type | http://purl.uniprot.org/core/Journal_Citation |
| http://purl.uniprot.org/citations/21768390 | http://www.w3.org/2000/01/rdf-schema#comment | "Sequence-specific gene silencing by short hairpin (sh) RNAs has recently emerged as an indispensable tool for understanding gene function and a promising avenue for drug discovery. However, a wider biomedical use of this approach is hindered by the lack of straightforward methods for achieving uniform expression of shRNAs in mammalian cell cultures. Here we report a high-efficiency and low-background (HILO) recombination-mediated cassette exchange (RMCE) technology that yields virtually homogeneous cell pools containing doxycycline-inducible shRNA elements in a matter of days and with minimal efforts. To ensure immediate utility of this approach for a wider research community, we modified 11 commonly used human (A549, HT1080, HEK293T, HeLa, HeLa-S3, and U2OS) and mouse (CAD, L929, N2a, NIH 3T3, and P19) cell lines to be compatible with the HILO-RMCE process. Because of its technical simplicity and cost efficiency, the technology will be advantageous for both low- and high-throughput shRNA experiments. We also provide evidence that HILO-RMCE will facilitate a wider range of molecular and cell biology applications by allowing one to rapidly engineer cell populations expressing essentially any transgene of interest."xsd:string |
| http://purl.uniprot.org/citations/21768390 | http://purl.org/dc/terms/identifier | "doi:10.1073/pnas.1103532108"xsd:string |
| http://purl.uniprot.org/citations/21768390 | http://purl.uniprot.org/core/author | "Makeyev E.V."xsd:string |
| http://purl.uniprot.org/citations/21768390 | http://purl.uniprot.org/core/author | "Yap K."xsd:string |
| http://purl.uniprot.org/citations/21768390 | http://purl.uniprot.org/core/author | "Khandelia P."xsd:string |
| http://purl.uniprot.org/citations/21768390 | http://purl.uniprot.org/core/date | "2011"xsd:gYear |
| http://purl.uniprot.org/citations/21768390 | http://purl.uniprot.org/core/name | "Proc Natl Acad Sci U S A"xsd:string |
| http://purl.uniprot.org/citations/21768390 | http://purl.uniprot.org/core/pages | "12799-12804"xsd:string |
| http://purl.uniprot.org/citations/21768390 | http://purl.uniprot.org/core/title | "Streamlined platform for short hairpin RNA interference and transgenesis in cultured mammalian cells."xsd:string |
| http://purl.uniprot.org/citations/21768390 | http://purl.uniprot.org/core/volume | "108"xsd:string |
| http://purl.uniprot.org/citations/21768390 | http://www.w3.org/2004/02/skos/core#exactMatch | http://purl.uniprot.org/pubmed/21768390 |
| http://purl.uniprot.org/citations/21768390 | http://xmlns.com/foaf/0.1/primaryTopicOf | https://pubmed.ncbi.nlm.nih.gov/21768390 |
| http://purl.uniprot.org/uniprot/#_A0A0G2JGW0-mappedCitation-21768390 | http://www.w3.org/1999/02/22-rdf-syntax-ns#object | http://purl.uniprot.org/citations/21768390 |
| http://purl.uniprot.org/uniprot/#_A0A0G2JE77-mappedCitation-21768390 | http://www.w3.org/1999/02/22-rdf-syntax-ns#object | http://purl.uniprot.org/citations/21768390 |
| http://purl.uniprot.org/uniprot/#_A0A0G2JEB9-mappedCitation-21768390 | http://www.w3.org/1999/02/22-rdf-syntax-ns#object | http://purl.uniprot.org/citations/21768390 |
| http://purl.uniprot.org/uniprot/#_E9Q0W3-mappedCitation-21768390 | http://www.w3.org/1999/02/22-rdf-syntax-ns#object | http://purl.uniprot.org/citations/21768390 |
| http://purl.uniprot.org/uniprot/#_A0A0G2JFV8-mappedCitation-21768390 | http://www.w3.org/1999/02/22-rdf-syntax-ns#object | http://purl.uniprot.org/citations/21768390 |
| http://purl.uniprot.org/uniprot/#_E9Q279-mappedCitation-21768390 | http://www.w3.org/1999/02/22-rdf-syntax-ns#object | http://purl.uniprot.org/citations/21768390 |
| http://purl.uniprot.org/uniprot/#_E9QMW9-mappedCitation-21768390 | http://www.w3.org/1999/02/22-rdf-syntax-ns#object | http://purl.uniprot.org/citations/21768390 |
| http://purl.uniprot.org/uniprot/#_F7AXP1-mappedCitation-21768390 | http://www.w3.org/1999/02/22-rdf-syntax-ns#object | http://purl.uniprot.org/citations/21768390 |
| http://purl.uniprot.org/uniprot/#_F7DCW4-mappedCitation-21768390 | http://www.w3.org/1999/02/22-rdf-syntax-ns#object | http://purl.uniprot.org/citations/21768390 |
| http://purl.uniprot.org/uniprot/#_E9PZ39-mappedCitation-21768390 | http://www.w3.org/1999/02/22-rdf-syntax-ns#object | http://purl.uniprot.org/citations/21768390 |
| http://purl.uniprot.org/uniprot/#_Q8CB58-mappedCitation-21768390 | http://www.w3.org/1999/02/22-rdf-syntax-ns#object | http://purl.uniprot.org/citations/21768390 |
| http://purl.uniprot.org/uniprot/#_P17225-mappedCitation-21768390 | http://www.w3.org/1999/02/22-rdf-syntax-ns#object | http://purl.uniprot.org/citations/21768390 |