| http://purl.uniprot.org/citations/21769867 | http://www.w3.org/1999/02/22-rdf-syntax-ns#type | http://purl.uniprot.org/core/Journal_Citation |
| http://purl.uniprot.org/citations/21769867 | http://www.w3.org/2000/01/rdf-schema#comment | "MicroRNAs play an important role in myocardial diseases. MiR-133a regulates cardiac hypertrophy, while miR-29b is involved in cardiac fibrosis. The aim of this study was to evaluate whether miR-133a and miR-29b play a role in myocardial fibrosis caused by Angiotensin II (Ang II)-dependent hypertension. Sprague-Dawley rats were treated for 4 weeks with Ang II (200 ng/kg/min) or Ang II + irbesartan (50 mg/kg/day in drinking water), or saline by osmotic minipumps. At the end of the experimental period, cardiac miR-133a and miR-29b expression was measured by real-time PCR, and myocardial fibrosis was evaluated by morphometric analysis. A computer-based prediction algorithm led to the identification of collagen 1a1 (Col1A1) as a putative target of miR-133a. A reporter plasmid bearing the 3'-untranslated regions (UTRs) of Col1A1 mRNA was constructed and luciferase assay was performed. MiR-133a suppressed the activity of luciferase when the reporter gene was linked to a 3'-UTR segment of Col1A1 (P < 0.01). Mutation of miR-133a binding sites in the 3'-UTR of Col1A1 mRNA abolished miR-133a-mediated repression of reporter gene activity, showing that Col1A1 is a real target of miR-133a. In vivo, Ang II caused an increase in systolic blood pressure (P < 0.0001, tail cuff) and myocardial fibrosis in presence of a decrease in miR-133a (P < 0.01) and miR-29b (P < 0.01), and an increase in Col1A1 expression (P < 0.01). These effects were abolished by Ang II administration + irbesartan. These data demonstrate a relationship between miR-133a and Col1A1, suggesting that myocardial fibrosis occurring in Ang II-dependent hypertension is regulated by the down-regulation of miR-133a and miR-29b through the modulation of Col1A1 expression."xsd:string |
| http://purl.uniprot.org/citations/21769867 | http://purl.org/dc/terms/identifier | "doi:10.1002/jcp.22939"xsd:string |
| http://purl.uniprot.org/citations/21769867 | http://purl.uniprot.org/core/author | "Stella A."xsd:string |
| http://purl.uniprot.org/citations/21769867 | http://purl.uniprot.org/core/author | "Zerbini G."xsd:string |
| http://purl.uniprot.org/citations/21769867 | http://purl.uniprot.org/core/author | "Condorelli G."xsd:string |
| http://purl.uniprot.org/citations/21769867 | http://purl.uniprot.org/core/author | "Mancini M."xsd:string |
| http://purl.uniprot.org/citations/21769867 | http://purl.uniprot.org/core/author | "Catalucci D."xsd:string |
| http://purl.uniprot.org/citations/21769867 | http://purl.uniprot.org/core/author | "Leopizzi M."xsd:string |
| http://purl.uniprot.org/citations/21769867 | http://purl.uniprot.org/core/author | "Bombardi C."xsd:string |
| http://purl.uniprot.org/citations/21769867 | http://purl.uniprot.org/core/author | "Corradi B."xsd:string |
| http://purl.uniprot.org/citations/21769867 | http://purl.uniprot.org/core/author | "Castoldi G."xsd:string |
| http://purl.uniprot.org/citations/21769867 | http://purl.uniprot.org/core/author | "Di Gioia C.R."xsd:string |
| http://purl.uniprot.org/citations/21769867 | http://purl.uniprot.org/core/author | "Gualazzi M.G."xsd:string |
| http://purl.uniprot.org/citations/21769867 | http://purl.uniprot.org/core/date | "2012"xsd:gYear |
| http://purl.uniprot.org/citations/21769867 | http://purl.uniprot.org/core/name | "J Cell Physiol"xsd:string |
| http://purl.uniprot.org/citations/21769867 | http://purl.uniprot.org/core/pages | "850-856"xsd:string |
| http://purl.uniprot.org/citations/21769867 | http://purl.uniprot.org/core/title | "MiR-133a regulates collagen 1A1: potential role of miR-133a in myocardial fibrosis in angiotensin II-dependent hypertension."xsd:string |
| http://purl.uniprot.org/citations/21769867 | http://purl.uniprot.org/core/volume | "227"xsd:string |
| http://purl.uniprot.org/citations/21769867 | http://www.w3.org/2004/02/skos/core#exactMatch | http://purl.uniprot.org/pubmed/21769867 |
| http://purl.uniprot.org/citations/21769867 | http://xmlns.com/foaf/0.1/primaryTopicOf | https://pubmed.ncbi.nlm.nih.gov/21769867 |
| http://purl.uniprot.org/uniprot/P29089#attribution-85FA1F6594562301022F74E02C96BA52 | http://purl.uniprot.org/core/source | http://purl.uniprot.org/citations/21769867 |
| http://purl.uniprot.org/uniprot/P25095#attribution-85FA1F6594562301022F74E02C96BA52 | http://purl.uniprot.org/core/source | http://purl.uniprot.org/citations/21769867 |
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| http://purl.uniprot.org/uniprot/#_A0A8I5ZRN2-mappedCitation-21769867 | http://www.w3.org/1999/02/22-rdf-syntax-ns#object | http://purl.uniprot.org/citations/21769867 |