| http://purl.uniprot.org/citations/21772247 | http://www.w3.org/1999/02/22-rdf-syntax-ns#type | http://purl.uniprot.org/core/Journal_Citation |
| http://purl.uniprot.org/citations/21772247 | http://www.w3.org/2000/01/rdf-schema#comment | "The spindle assembly checkpoint (SAC) restrains anaphase until all chromosomes become bi-oriented on the mitotic spindle. The SAC protein Mad2 can fold into two distinct conformers, open (O) and closed (C), and can asymmetrically dimerize. Here, we describe a monoclonal antibody that specifically recognizes the dimerization interface of C-Mad2. This antibody revealed several conformation-specific features of Mad2 in human cells. Notably, we show that Mad2 requires association with Mad1 to adopt the closed conformation and that the activity of the Mad1:C-Mad2 complex undergoes regulation by p31comet-dependent 'capping'. Furthermore, C-Mad2 antibody microinjection caused an abrupt termination of the SAC and accelerated mitotic progression. Remarkably, microinjection of a Mad1-neutralizing antibody triggered a comparable mitotic acceleration. Our study provides direct in vivo evidence for the model that a kinetochore complex of Mad1:C-Mad2 acts as a template to sustain the SAC and it challenges the distinction between SAC and mitotic timer."xsd:string |
| http://purl.uniprot.org/citations/21772247 | http://purl.org/dc/terms/identifier | "doi:10.1038/emboj.2011.239"xsd:string |
| http://purl.uniprot.org/citations/21772247 | http://purl.uniprot.org/core/author | "Nigg E.A."xsd:string |
| http://purl.uniprot.org/citations/21772247 | http://purl.uniprot.org/core/author | "Santamaria A."xsd:string |
| http://purl.uniprot.org/citations/21772247 | http://purl.uniprot.org/core/author | "Kaulich M."xsd:string |
| http://purl.uniprot.org/citations/21772247 | http://purl.uniprot.org/core/author | "Fava L.L."xsd:string |
| http://purl.uniprot.org/citations/21772247 | http://purl.uniprot.org/core/date | "2011"xsd:gYear |
| http://purl.uniprot.org/citations/21772247 | http://purl.uniprot.org/core/name | "EMBO J"xsd:string |
| http://purl.uniprot.org/citations/21772247 | http://purl.uniprot.org/core/pages | "3322-3336"xsd:string |
| http://purl.uniprot.org/citations/21772247 | http://purl.uniprot.org/core/title | "Probing the in vivo function of Mad1:C-Mad2 in the spindle assembly checkpoint."xsd:string |
| http://purl.uniprot.org/citations/21772247 | http://purl.uniprot.org/core/volume | "30"xsd:string |
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