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http://purl.uniprot.org/citations/21780504http://www.w3.org/1999/02/22-rdf-syntax-ns#typehttp://purl.uniprot.org/core/Journal_Citation
http://purl.uniprot.org/citations/21780504http://www.w3.org/1999/02/22-rdf-syntax-ns#typehttp://purl.uniprot.org/core/Journal_Citation
http://purl.uniprot.org/citations/21780504http://www.w3.org/2000/01/rdf-schema#comment"We cloned an intronless, nuclear-encoded opsin gene from an EST library of Acetabularia acetabulum. Acetabularia rhodopsin I (ARI) encodes a protein of 246 amino acids with molecular weight of 27 kDa. ARI was reconstituted in the Xenopus oocyte expression system to characterize its electrophysiological properties utilizing the two-electrode voltage-clamping technique. Oocytes where ARI cRNA was injected displayed outward directed currents in response to light. The maximum action spectrum of ARI was detected at 520 nm green light. Light-stimulated ARI current amplitude was altered by the protons, but not by the other ions in recording solutions, suggesting that the algal rhodopsin is a light-stimulated proton pump. Typical proton-mediated outward current elicited by 520 nm light was characterized with two phases of non-inactivating outward current following initial transient current. Taken together, we here reported cloning of a novel Acetabularia opsin gene which was characterized to be a proton-pump stimulated by light."xsd:string
http://purl.uniprot.org/citations/21780504http://purl.org/dc/terms/identifier"doi:10.1166/jnn.2011.3650"xsd:string
http://purl.uniprot.org/citations/21780504http://purl.org/dc/terms/identifier"doi:10.1166/jnn.2011.3650"xsd:string
http://purl.uniprot.org/citations/21780504http://purl.uniprot.org/core/author"Lee J.H."xsd:string
http://purl.uniprot.org/citations/21780504http://purl.uniprot.org/core/author"Lee S.S."xsd:string
http://purl.uniprot.org/citations/21780504http://purl.uniprot.org/core/author"Kang H.-W."xsd:string
http://purl.uniprot.org/citations/21780504http://purl.uniprot.org/core/author"Kim S.Y."xsd:string
http://purl.uniprot.org/citations/21780504http://purl.uniprot.org/core/author"Kim S.Y."xsd:string
http://purl.uniprot.org/citations/21780504http://purl.uniprot.org/core/author"Lee J.-H."xsd:string
http://purl.uniprot.org/citations/21780504http://purl.uniprot.org/core/author"Jung K.-H."xsd:string
http://purl.uniprot.org/citations/21780504http://purl.uniprot.org/core/author"Jung K.H."xsd:string
http://purl.uniprot.org/citations/21780504http://purl.uniprot.org/core/author"Kang H.W."xsd:string
http://purl.uniprot.org/citations/21780504http://purl.uniprot.org/core/author"Choi A.R."xsd:string
http://purl.uniprot.org/citations/21780504http://purl.uniprot.org/core/author"Choi A.R."xsd:string
http://purl.uniprot.org/citations/21780504http://purl.uniprot.org/core/author"Lee S.-S."xsd:string
http://purl.uniprot.org/citations/21780504http://purl.uniprot.org/core/date"2011"xsd:gYear
http://purl.uniprot.org/citations/21780504http://purl.uniprot.org/core/date"2011"xsd:gYear
http://purl.uniprot.org/citations/21780504http://purl.uniprot.org/core/name"J. Nanosci. Nanotechnol."xsd:string
http://purl.uniprot.org/citations/21780504http://purl.uniprot.org/core/name"J Nanosci Nanotechnol"xsd:string
http://purl.uniprot.org/citations/21780504http://purl.uniprot.org/core/pages"4596-4600"xsd:string
http://purl.uniprot.org/citations/21780504http://purl.uniprot.org/core/pages"4596-4600"xsd:string
http://purl.uniprot.org/citations/21780504http://purl.uniprot.org/core/title"Acetabularia rhodopsin I is a light-stimulated proton pump."xsd:string
http://purl.uniprot.org/citations/21780504http://purl.uniprot.org/core/title"Acetabularia rhodopsin I is a light-stimulated proton pump."xsd:string