| http://purl.uniprot.org/citations/21782856 | http://www.w3.org/1999/02/22-rdf-syntax-ns#type | http://purl.uniprot.org/core/Journal_Citation |
| http://purl.uniprot.org/citations/21782856 | http://www.w3.org/2000/01/rdf-schema#comment | "Parcs/Gpn3 is a putative GTPase that is conserved in eukaryotic cells from yeast to humans, suggesting that it plays a fundamental, but still unknown, cellular function. Suppression of Parcs/Gpn3 expression by RNAi completely blocked cell proliferation in MCF-12A cells and other mammary epithelial cell lines. Unexpectedly, Parcs/Gpn3 knockdown had a more modest effect in the proliferation of the tumorigenic MDA-MB-231 and SK-BR3 cells. RNA polymerase II (RNAP II) co-immunoprecipitated with Parcs/Gpn3. Parcs/Gpn3 depletion caused a reduction in overall RNA synthesis in MCF-12A cells but not in MDA-MB-231 cells, demonstrating a role for Parcs/Gpn3 in transcription, and pointing to a defect in RNA synthesis by RNAP II as the possible cause of halted proliferation. The absence of Parcs/Gpn3 in MCF-12A cells caused a dramatic change in the sub-cellular localization of Rpb1, the largest subunit of RNAP II. As expected, Rpb1 was present only in the nucleus of MCF-12A control cells, whereas in Parcs/Gpn3-depleted MCF-12A cells, Rpb1 was detected exclusively in the cytoplasm. This effect was specific, as histones remained nuclear independently of Parcs/Gpn3. Rpb1 protein levels were markedly increased in Parcs/Gpn3-depleted MCF-12A cells. Interestingly, Rpb1 distribution was only marginally affected after knocking-down Parcs/Gpn3 in MDA-MB-231 cells. In conclusion, we report here, for the first time, that Parcs/Gpn3 plays a critical role in the nuclear accumulation of RNAP II, and we propose that this function explains the relative importance of Parcs/Gpn3 in cell proliferation. Intriguingly, at least some tumorigenic mammary cells have evolved mechanisms that allow them to proliferate in a Parcs/Gpn3-independent manner."xsd:string |
| http://purl.uniprot.org/citations/21782856 | http://purl.org/dc/terms/identifier | "doi:10.1016/j.bbamcr.2011.07.005"xsd:string |
| http://purl.uniprot.org/citations/21782856 | http://purl.uniprot.org/core/author | "Calera M.R."xsd:string |
| http://purl.uniprot.org/citations/21782856 | http://purl.uniprot.org/core/author | "Sanchez-Olea R."xsd:string |
| http://purl.uniprot.org/citations/21782856 | http://purl.uniprot.org/core/author | "Araiza-Villanueva M.G."xsd:string |
| http://purl.uniprot.org/citations/21782856 | http://purl.uniprot.org/core/author | "Castellanos-Teran F."xsd:string |
| http://purl.uniprot.org/citations/21782856 | http://purl.uniprot.org/core/author | "Moreno-Aguilar C.A."xsd:string |
| http://purl.uniprot.org/citations/21782856 | http://purl.uniprot.org/core/author | "Pena-Gomez S.G."xsd:string |
| http://purl.uniprot.org/citations/21782856 | http://purl.uniprot.org/core/author | "Robledo-Rivera A.Y."xsd:string |
| http://purl.uniprot.org/citations/21782856 | http://purl.uniprot.org/core/author | "Zamora-Ramos C."xsd:string |
| http://purl.uniprot.org/citations/21782856 | http://purl.uniprot.org/core/date | "2011"xsd:gYear |
| http://purl.uniprot.org/citations/21782856 | http://purl.uniprot.org/core/name | "Biochim Biophys Acta"xsd:string |
| http://purl.uniprot.org/citations/21782856 | http://purl.uniprot.org/core/pages | "1708-1716"xsd:string |
| http://purl.uniprot.org/citations/21782856 | http://purl.uniprot.org/core/title | "Parcs/Gpn3 is required for the nuclear accumulation of RNA polymerase II."xsd:string |
| http://purl.uniprot.org/citations/21782856 | http://purl.uniprot.org/core/volume | "1813"xsd:string |
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