| http://purl.uniprot.org/citations/21790537 | http://www.w3.org/1999/02/22-rdf-syntax-ns#type | http://purl.uniprot.org/core/Journal_Citation |
| http://purl.uniprot.org/citations/21790537 | http://www.w3.org/2000/01/rdf-schema#comment | "BACKGROUND AND PURPOSE The Na(+) /Ca(2+) exchanger is a bi-directional transporter that plays an important role in maintaining the concentration of cytosolic Ca(2+) ([Ca(2+) ](i) ) of quiescent platelets and increasing it during activation with some, but not all, agonists. There are two classes of Na(+) /Ca(2+) exchangers: K(+) -independent Na(+) /Ca(2+) exchanger (NCX) and K(+) -dependent Na(+) /Ca(2+) exchanger (NCKX). Platelets have previously been shown to express NCKX1. However, initial studies from our laboratory suggest that NCX may also play a role in platelet activation. The objective of this study was to determine if the human platelet expresses functional NCXs. EXPERIMENTAL APPROACH RT-PCR, DNA sequencing and Western blot analysis were utilized to characterize the human platelet Na(+) /Ca(2+) exchangers. Their function during quiescence and collagen-induced activation was determined by measuring [Ca(2+) ](i) with calcium-green/fura-red in response to: changes in the Na(+) and K(+) gradient, NCX pharmacological inhibitors (CBDMB, KB-R7943 and SEA0400) and antibodies specific to extracellular epitopes of the exchangers. KEY RESULTS Human platelets express NCX1.3, NCX3.2 and NCX3.4. The NCXs operate in the Ca(2+) efflux mode in resting platelets and also during their activation with thrombin but not collagen. Collagen-induced increase in [Ca(2+) ](i) was reduced with the pharmacological inhibitors of NCX (CBDMB, KB-R7943 or SEA0400), anti-NCX1 and anti-NCX3. In contrast, anti-NCKX1 enhanced the collagen-induced increase in [Ca(2+) ](i) . CONCLUSIONS AND IMPLICATIONS Human platelets express K(+) -independent Na(+) /Ca(2+) exchangers NCX1.3, NCX3.2 and NCX3.4. During collagen activation, NCX1 and NCX3 transiently reverse to promote Ca(2+) influx, whereas NCKX1 continues to operate in the Ca(2+) efflux mode to reduce [Ca(2+) ](i) ."xsd:string |
| http://purl.uniprot.org/citations/21790537 | http://purl.org/dc/terms/identifier | "doi:10.1111/j.1476-5381.2011.01600.x"xsd:string |
| http://purl.uniprot.org/citations/21790537 | http://purl.uniprot.org/core/author | "Matsuda T."xsd:string |
| http://purl.uniprot.org/citations/21790537 | http://purl.uniprot.org/core/author | "Bose R."xsd:string |
| http://purl.uniprot.org/citations/21790537 | http://purl.uniprot.org/core/author | "Roberts D.E."xsd:string |
| http://purl.uniprot.org/citations/21790537 | http://purl.uniprot.org/core/date | "2012"xsd:gYear |
| http://purl.uniprot.org/citations/21790537 | http://purl.uniprot.org/core/name | "Br J Pharmacol"xsd:string |
| http://purl.uniprot.org/citations/21790537 | http://purl.uniprot.org/core/pages | "922-936"xsd:string |
| http://purl.uniprot.org/citations/21790537 | http://purl.uniprot.org/core/title | "Molecular and functional characterization of the human platelet Na(+) /Ca(2+) exchangers."xsd:string |
| http://purl.uniprot.org/citations/21790537 | http://purl.uniprot.org/core/volume | "165"xsd:string |
| http://purl.uniprot.org/citations/21790537 | http://www.w3.org/2004/02/skos/core#exactMatch | http://purl.uniprot.org/pubmed/21790537 |
| http://purl.uniprot.org/citations/21790537 | http://xmlns.com/foaf/0.1/primaryTopicOf | https://pubmed.ncbi.nlm.nih.gov/21790537 |
| http://purl.uniprot.org/uniprot/#_Q14913-mappedCitation-21790537 | http://www.w3.org/1999/02/22-rdf-syntax-ns#object | http://purl.uniprot.org/citations/21790537 |
| http://purl.uniprot.org/uniprot/#_Q4QQH3-mappedCitation-21790537 | http://www.w3.org/1999/02/22-rdf-syntax-ns#object | http://purl.uniprot.org/citations/21790537 |
| http://purl.uniprot.org/uniprot/#_P32418-mappedCitation-21790537 | http://www.w3.org/1999/02/22-rdf-syntax-ns#object | http://purl.uniprot.org/citations/21790537 |
| http://purl.uniprot.org/uniprot/#_Q7Z686-mappedCitation-21790537 | http://www.w3.org/1999/02/22-rdf-syntax-ns#object | http://purl.uniprot.org/citations/21790537 |
| http://purl.uniprot.org/uniprot/#_Q6LAJ8-mappedCitation-21790537 | http://www.w3.org/1999/02/22-rdf-syntax-ns#object | http://purl.uniprot.org/citations/21790537 |
| http://purl.uniprot.org/uniprot/#_Q6LAJ9-mappedCitation-21790537 | http://www.w3.org/1999/02/22-rdf-syntax-ns#object | http://purl.uniprot.org/citations/21790537 |
| http://purl.uniprot.org/uniprot/P32418 | http://purl.uniprot.org/core/mappedCitation | http://purl.uniprot.org/citations/21790537 |
| http://purl.uniprot.org/uniprot/Q14913 | http://purl.uniprot.org/core/mappedCitation | http://purl.uniprot.org/citations/21790537 |
| http://purl.uniprot.org/uniprot/Q6LAJ9 | http://purl.uniprot.org/core/mappedCitation | http://purl.uniprot.org/citations/21790537 |
| http://purl.uniprot.org/uniprot/Q6LAJ8 | http://purl.uniprot.org/core/mappedCitation | http://purl.uniprot.org/citations/21790537 |
| http://purl.uniprot.org/uniprot/Q4QQH3 | http://purl.uniprot.org/core/mappedCitation | http://purl.uniprot.org/citations/21790537 |
| http://purl.uniprot.org/uniprot/Q7Z686 | http://purl.uniprot.org/core/mappedCitation | http://purl.uniprot.org/citations/21790537 |