| http://purl.uniprot.org/citations/21813449 | http://www.w3.org/1999/02/22-rdf-syntax-ns#type | http://purl.uniprot.org/core/Journal_Citation |
| http://purl.uniprot.org/citations/21813449 | http://www.w3.org/1999/02/22-rdf-syntax-ns#type | http://purl.uniprot.org/core/Journal_Citation |
| http://purl.uniprot.org/citations/21813449 | http://www.w3.org/2000/01/rdf-schema#comment | "Protein palmitoylation is a dynamic process that regulates membrane targeting of proteins and protein-protein interactions. We have previously demonstrated a critical role for protein palmitoylation in platelet activation and have identified palmitoylation machinery in platelets. Using a novel proteomic approach, Palmitoyl Protein Identification and Site Characterization, we have begun to characterize the human platelet palmitoylome. Palmitoylated proteins were enriched from membranes isolated from resting platelets using acyl-biotinyl exchange chemistry, followed by identification using liquid chromatography-tandem mass spectrometry. This global analysis identified > 1300 proteins, of which 215 met criteria for significance and represent the platelet palmitoylome. This collection includes 51 known palmitoylated proteins, 61 putative palmitoylated proteins identified in other palmitoylation-specific proteomic studies, and 103 new putative palmitoylated proteins. Of these candidates, we chose to validate the palmitoylation of triggering receptors expressed on myeloid cell (TREM)-like transcript-1 (TLT-1) as its expression is restricted to platelets and megakaryocytes. We determined that TLT-1 is a palmitoylated protein using metabolic labeling with [³H]palmitate and identified the site of TLT-1 palmitoylation as cysteine 196. The discovery of new platelet palmitoyl protein candidates will provide a resource for subsequent investigations to validate the palmitoylation of these proteins and to determine the role palmitoylation plays in their function."xsd:string |
| http://purl.uniprot.org/citations/21813449 | http://purl.org/dc/terms/identifier | "doi:10.1182/blood-2011-05-353078"xsd:string |
| http://purl.uniprot.org/citations/21813449 | http://purl.org/dc/terms/identifier | "doi:10.1182/blood-2011-05-353078"xsd:string |
| http://purl.uniprot.org/citations/21813449 | http://purl.uniprot.org/core/author | "Yang W."xsd:string |
| http://purl.uniprot.org/citations/21813449 | http://purl.uniprot.org/core/author | "Yang W."xsd:string |
| http://purl.uniprot.org/citations/21813449 | http://purl.uniprot.org/core/author | "Steen H."xsd:string |
| http://purl.uniprot.org/citations/21813449 | http://purl.uniprot.org/core/author | "Steen H."xsd:string |
| http://purl.uniprot.org/citations/21813449 | http://purl.uniprot.org/core/author | "Freeman M.R."xsd:string |
| http://purl.uniprot.org/citations/21813449 | http://purl.uniprot.org/core/author | "Freeman M.R."xsd:string |
| http://purl.uniprot.org/citations/21813449 | http://purl.uniprot.org/core/author | "Flaumenhaft R."xsd:string |
| http://purl.uniprot.org/citations/21813449 | http://purl.uniprot.org/core/author | "Flaumenhaft R."xsd:string |
| http://purl.uniprot.org/citations/21813449 | http://purl.uniprot.org/core/author | "Dowal L."xsd:string |
| http://purl.uniprot.org/citations/21813449 | http://purl.uniprot.org/core/author | "Dowal L."xsd:string |
| http://purl.uniprot.org/citations/21813449 | http://purl.uniprot.org/core/date | "2011"xsd:gYear |
| http://purl.uniprot.org/citations/21813449 | http://purl.uniprot.org/core/date | "2011"xsd:gYear |
| http://purl.uniprot.org/citations/21813449 | http://purl.uniprot.org/core/name | "Blood"xsd:string |
| http://purl.uniprot.org/citations/21813449 | http://purl.uniprot.org/core/name | "Blood"xsd:string |
| http://purl.uniprot.org/citations/21813449 | http://purl.uniprot.org/core/pages | "E62-E73"xsd:string |
| http://purl.uniprot.org/citations/21813449 | http://purl.uniprot.org/core/pages | "E62-E73"xsd:string |
| http://purl.uniprot.org/citations/21813449 | http://purl.uniprot.org/core/title | "Proteomic analysis of palmitoylated platelet proteins."xsd:string |
| http://purl.uniprot.org/citations/21813449 | http://purl.uniprot.org/core/title | "Proteomic analysis of palmitoylated platelet proteins."xsd:string |
| http://purl.uniprot.org/citations/21813449 | http://purl.uniprot.org/core/volume | "118"xsd:string |
| http://purl.uniprot.org/citations/21813449 | http://purl.uniprot.org/core/volume | "118"xsd:string |