http://purl.uniprot.org/citations/2182221 | http://www.w3.org/1999/02/22-rdf-syntax-ns#type | http://purl.uniprot.org/core/Journal_Citation |
http://purl.uniprot.org/citations/2182221 | http://www.w3.org/2000/01/rdf-schema#comment | "Total mitochondrial aspartate aminotransferase (EC 2.6.1.1), the sum of apo- and holo-mitochondrial aspartate aminotransferase activity in human serum, was measured by using a proteolytic method: inactivation of cytosolic aspartate aminotransferase with cytosolic aspartate aminotransferase-inactivating protease 401 from Streptomyces violaceochromogenes. Cytosolic aspartate aminotransferase is completely inactivated, and apo-mitochondrial aspartate aminotransferase is completely activated by pyridoxal 5'-phosphate within 5 min. Results by the proposed method correlated well with those by an immunochemical method (r = 0.994, n = 145) and showed excellent inhibitory activity of the protease for holo- and apo-cytosolic aspartate aminotransferase up to 5000 U/L and activation of mitochondrial apo-aspartate aminotransferase up to 2000 U/L in the presence of 100 mumol of pyridoxal 5'-phosphate per liter. Within-run Cvs were good (1.13-7.49%). Mean values for total mitochondrial aspartate aminotransferase and apo-mitochondrial aspartate aminotransferase activities in serum of the healthy subjects were 4.8 (SD 0.9) and 1.8 (SD 0.8) U/L, respectively (n = 154). Various common interferents tested did not affect this assay."xsd:string |
http://purl.uniprot.org/citations/2182221 | http://purl.uniprot.org/core/author | "Okabe H."xsd:string |
http://purl.uniprot.org/citations/2182221 | http://purl.uniprot.org/core/author | "Murao S."xsd:string |
http://purl.uniprot.org/citations/2182221 | http://purl.uniprot.org/core/author | "Uji Y."xsd:string |
http://purl.uniprot.org/citations/2182221 | http://purl.uniprot.org/core/author | "Sugiuchi H."xsd:string |
http://purl.uniprot.org/citations/2182221 | http://purl.uniprot.org/core/author | "Watazu Y."xsd:string |
http://purl.uniprot.org/citations/2182221 | http://purl.uniprot.org/core/date | "1990"xsd:gYear |
http://purl.uniprot.org/citations/2182221 | http://purl.uniprot.org/core/name | "Clin Chem"xsd:string |
http://purl.uniprot.org/citations/2182221 | http://purl.uniprot.org/core/pages | "687-689"xsd:string |
http://purl.uniprot.org/citations/2182221 | http://purl.uniprot.org/core/title | "New automated measurement of mitochondrial aspartate aminotransferase with use of protease 401."xsd:string |
http://purl.uniprot.org/citations/2182221 | http://purl.uniprot.org/core/volume | "36"xsd:string |
http://purl.uniprot.org/citations/2182221 | http://www.w3.org/2004/02/skos/core#exactMatch | http://purl.uniprot.org/pubmed/2182221 |
http://purl.uniprot.org/citations/2182221 | http://xmlns.com/foaf/0.1/primaryTopicOf | https://pubmed.ncbi.nlm.nih.gov/2182221 |
http://purl.uniprot.org/uniprot/P17174#attribution-001F4C7A51EBE111178275DDF94FE5B0 | http://purl.uniprot.org/core/source | http://purl.uniprot.org/citations/2182221 |
http://purl.uniprot.org/uniprot/P00505#attribution-001F4C7A51EBE111178275DDF94FE5B0 | http://purl.uniprot.org/core/source | http://purl.uniprot.org/citations/2182221 |