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http://purl.uniprot.org/citations/21827498http://www.w3.org/1999/02/22-rdf-syntax-ns#typehttp://purl.uniprot.org/core/Journal_Citation
http://purl.uniprot.org/citations/21827498http://www.w3.org/2000/01/rdf-schema#comment"

Objectives

Honokiol is a small-molecule polyphenol isolated from the species Magnolia obovata. We hypothesized that honokiol attenuated vascular contractions through the inhibition of the RhoA/Rho-kinase signalling pathway.

Methods

Rat aortic rings were denuded of endothelium, mounted in organ baths, and subjected to contraction or relaxation. Phosphorylation of 20kDa myosin light chains (MLC(20) ), myosin phosphatase targeting subunit 1 (MYPT1) and protein kinase C (PKC)-potentiated inhibitory protein for heterotrimeric myosin light chain phosphatase (MLCP) of 17kDa (CPI17) were examined by immunoblot. We also measured the amount of guanosine triphosphate RhoA as a marker for RhoA activation.

Key findings

Pretreatment with honokiol dose-dependently inhibited the concentration-response curves in response to sodium fluoride (NaF) or thromboxane A(2) agonist U46619. Honokiol decreased the phosphorylation levels of MLC(20) , MYPT1(Thr855) and CPI17(Thr38) as well as the activation of RhoA induced by 8.0mm NaF or 30nm U46619.

Conclusions

These results demonstrated that honokiol attenuated vascular contraction through the inhibition of the RhoA/Rho-kinase signalling pathway."xsd:string
http://purl.uniprot.org/citations/21827498http://purl.org/dc/terms/identifier"doi:10.1111/j.2042-7158.2011.01332.x"xsd:string
http://purl.uniprot.org/citations/21827498http://purl.uniprot.org/core/author"Cho H.J."xsd:string
http://purl.uniprot.org/citations/21827498http://purl.uniprot.org/core/author"Kim I.K."xsd:string
http://purl.uniprot.org/citations/21827498http://purl.uniprot.org/core/author"Cha B.Y."xsd:string
http://purl.uniprot.org/citations/21827498http://purl.uniprot.org/core/author"Woo J.T."xsd:string
http://purl.uniprot.org/citations/21827498http://purl.uniprot.org/core/author"Seok Y.M."xsd:string
http://purl.uniprot.org/citations/21827498http://purl.uniprot.org/core/date"2011"xsd:gYear
http://purl.uniprot.org/citations/21827498http://purl.uniprot.org/core/name"J Pharm Pharmacol"xsd:string
http://purl.uniprot.org/citations/21827498http://purl.uniprot.org/core/pages"1244-1251"xsd:string
http://purl.uniprot.org/citations/21827498http://purl.uniprot.org/core/title"Honokiol attenuates vascular contraction through the inhibition of the RhoA/Rho-kinase signalling pathway in rat aortic rings."xsd:string
http://purl.uniprot.org/citations/21827498http://purl.uniprot.org/core/volume"63"xsd:string
http://purl.uniprot.org/citations/21827498http://www.w3.org/2004/02/skos/core#exactMatchhttp://purl.uniprot.org/pubmed/21827498
http://purl.uniprot.org/citations/21827498http://xmlns.com/foaf/0.1/primaryTopicOfhttps://pubmed.ncbi.nlm.nih.gov/21827498
http://purl.uniprot.org/uniprot/#_Q10728-mappedCitation-21827498http://www.w3.org/1999/02/22-rdf-syntax-ns#objecthttp://purl.uniprot.org/citations/21827498
http://purl.uniprot.org/uniprot/#_Q99MC0-mappedCitation-21827498http://www.w3.org/1999/02/22-rdf-syntax-ns#objecthttp://purl.uniprot.org/citations/21827498
http://purl.uniprot.org/uniprot/Q10728http://purl.uniprot.org/core/mappedCitationhttp://purl.uniprot.org/citations/21827498
http://purl.uniprot.org/uniprot/Q99MC0http://purl.uniprot.org/core/mappedCitationhttp://purl.uniprot.org/citations/21827498