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http://purl.uniprot.org/citations/21833587http://www.w3.org/1999/02/22-rdf-syntax-ns#typehttp://purl.uniprot.org/core/Journal_Citation
http://purl.uniprot.org/citations/21833587http://www.w3.org/2000/01/rdf-schema#comment"

Aims/hypothesis

The activation of platelet-derived growth factor receptor-β (PDGFR-β) signalling is increased in the glomeruli and tubules of diabetic animals. In this study, we examined the role of PDGFR-β signalling during the development of diabetic nephropathy.

Methods

We recently generated pancreatic beta cell-specific Ca(2+)/calmodulin-dependent protein kinase IIα (Thr286Asp) transgenic mice (CaMKIIα mice), which show very high plasma glucose levels up to 55.5 mmol/l and exhibit the features of diabetic nephropathy. These mice were crossed with conditional knockout mice in which Pdgfr-β (also known as Pdgfrb) was deleted postnatally. The effect of the deletion of the Pdgfr-β gene on diabetic nephropathy in CaMKIIα mice was evaluated at 10 and 16 weeks of age.

Results

The plasma glucose concentrations and HbA(1c) levels were elevated in the CaMKIIα mice from 4 weeks of age. Variables indicative of diabetic nephropathy, such as an increased urinary albumin/creatinine ratio, kidney weight/body weight ratio and mesangial area/glomerular area ratio, were observed at 16 weeks of age. The postnatal deletion of the Pdgfr-β gene significantly decreased the urinary albumin/creatinine ratio and mesangial area/glomerular area ratio without affecting the plasma glucose concentration. Furthermore, the increased oxidative stress in the kidneys of the CaMKIIα mice as shown by the increased urinary 8-hydroxydeoxyguanosine (8-OHdG) excretion and the increased expression of NAD(P)H oxidase 4 (NOX4), glutathione peroxidase 1 (GPX1) and manganese superoxide dismutase (MnSOD) was decreased by Pdgfr-β gene deletion.

Conclusions/interpretation

The activation of PDGFR-β signalling contributes to the progress of diabetic nephropathy, with an increase in oxidative stress and mesangial expansion in CaMKIIα mice."xsd:string
http://purl.uniprot.org/citations/21833587http://purl.org/dc/terms/identifier"doi:10.1007/s00125-011-2270-x"xsd:string
http://purl.uniprot.org/citations/21833587http://purl.uniprot.org/core/author"Kobayashi M."xsd:string
http://purl.uniprot.org/citations/21833587http://purl.uniprot.org/core/author"Ishii Y."xsd:string
http://purl.uniprot.org/citations/21833587http://purl.uniprot.org/core/author"Kato I."xsd:string
http://purl.uniprot.org/citations/21833587http://purl.uniprot.org/core/author"Okamoto H."xsd:string
http://purl.uniprot.org/citations/21833587http://purl.uniprot.org/core/author"Suzuki H."xsd:string
http://purl.uniprot.org/citations/21833587http://purl.uniprot.org/core/author"Yamazaki Y."xsd:string
http://purl.uniprot.org/citations/21833587http://purl.uniprot.org/core/author"Tobe K."xsd:string
http://purl.uniprot.org/citations/21833587http://purl.uniprot.org/core/author"Mahmood A."xsd:string
http://purl.uniprot.org/citations/21833587http://purl.uniprot.org/core/author"Takasawa S."xsd:string
http://purl.uniprot.org/citations/21833587http://purl.uniprot.org/core/author"Oya T."xsd:string
http://purl.uniprot.org/citations/21833587http://purl.uniprot.org/core/author"Kanatani Y."xsd:string
http://purl.uniprot.org/citations/21833587http://purl.uniprot.org/core/author"Sasahara M."xsd:string
http://purl.uniprot.org/citations/21833587http://purl.uniprot.org/core/author"Usui I."xsd:string
http://purl.uniprot.org/citations/21833587http://purl.uniprot.org/core/author"Senda S."xsd:string
http://purl.uniprot.org/citations/21833587http://purl.uniprot.org/core/author"Urakaze M."xsd:string
http://purl.uniprot.org/citations/21833587http://purl.uniprot.org/core/author"Fujisaka S."xsd:string
http://purl.uniprot.org/citations/21833587http://purl.uniprot.org/core/date"2011"xsd:gYear
http://purl.uniprot.org/citations/21833587http://purl.uniprot.org/core/name"Diabetologia"xsd:string
http://purl.uniprot.org/citations/21833587http://purl.uniprot.org/core/pages"2953-2962"xsd:string
http://purl.uniprot.org/citations/21833587http://purl.uniprot.org/core/title"Deletion of platelet-derived growth factor receptor-beta improves diabetic nephropathy in Ca^2⁺/calmodulin-dependent protein kinase IIalpha (Thr286Asp) transgenic mice."xsd:string
http://purl.uniprot.org/citations/21833587http://purl.uniprot.org/core/volume"54"xsd:string
http://purl.uniprot.org/citations/21833587http://www.w3.org/2004/02/skos/core#exactMatchhttp://purl.uniprot.org/pubmed/21833587
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