| http://purl.uniprot.org/citations/21835666 | http://www.w3.org/1999/02/22-rdf-syntax-ns#type | http://purl.uniprot.org/core/Journal_Citation |
| http://purl.uniprot.org/citations/21835666 | http://www.w3.org/1999/02/22-rdf-syntax-ns#type | http://purl.uniprot.org/core/Journal_Citation |
| http://purl.uniprot.org/citations/21835666 | http://www.w3.org/2000/01/rdf-schema#comment | "Tail-anchored (TA) proteins access the secretory pathway via posttranslational insertion of their C-terminal transmembrane domain into the endoplasmic reticulum (ER). Get3 is an ATPase that delivers TA proteins to the ER by interacting with the Get1-Get2 transmembrane complex, but how Get3's nucleotide cycle drives TA protein insertion remains unclear. Here, we establish that nucleotide binding to Get3 promotes Get3-TA protein complex formation by recruiting Get3 to a chaperone that hands over TA proteins to Get3. Biochemical reconstitution and mutagenesis reveal that the Get1-Get2 complex comprises the minimal TA protein insertion machinery with functionally critical cytosolic regions. By engineering a soluble heterodimer of Get1-Get2 cytosolic domains, we uncover the mechanism of TA protein release from Get3: Get2 tethers Get3-TA protein complexes into proximity with the ATPase-dependent, substrate-releasing activity of Get1. Lastly, we show that ATP enhances Get3 dissociation from the membrane, thus freeing Get1-Get2 for new rounds of substrate insertion."xsd:string |
| http://purl.uniprot.org/citations/21835666 | http://purl.org/dc/terms/identifier | "doi:10.1016/j.molcel.2011.07.020"xsd:string |
| http://purl.uniprot.org/citations/21835666 | http://purl.org/dc/terms/identifier | "doi:10.1016/j.molcel.2011.07.020"xsd:string |
| http://purl.uniprot.org/citations/21835666 | http://purl.uniprot.org/core/author | "Wang F."xsd:string |
| http://purl.uniprot.org/citations/21835666 | http://purl.uniprot.org/core/author | "Wang F."xsd:string |
| http://purl.uniprot.org/citations/21835666 | http://purl.uniprot.org/core/author | "Denic V."xsd:string |
| http://purl.uniprot.org/citations/21835666 | http://purl.uniprot.org/core/author | "Denic V."xsd:string |
| http://purl.uniprot.org/citations/21835666 | http://purl.uniprot.org/core/author | "Tung M."xsd:string |
| http://purl.uniprot.org/citations/21835666 | http://purl.uniprot.org/core/author | "Tung M."xsd:string |
| http://purl.uniprot.org/citations/21835666 | http://purl.uniprot.org/core/author | "Whynot A."xsd:string |
| http://purl.uniprot.org/citations/21835666 | http://purl.uniprot.org/core/author | "Whynot A."xsd:string |
| http://purl.uniprot.org/citations/21835666 | http://purl.uniprot.org/core/date | "2011"xsd:gYear |
| http://purl.uniprot.org/citations/21835666 | http://purl.uniprot.org/core/date | "2011"xsd:gYear |
| http://purl.uniprot.org/citations/21835666 | http://purl.uniprot.org/core/name | "Mol. Cell"xsd:string |
| http://purl.uniprot.org/citations/21835666 | http://purl.uniprot.org/core/name | "Mol. Cell"xsd:string |
| http://purl.uniprot.org/citations/21835666 | http://purl.uniprot.org/core/pages | "738-750"xsd:string |
| http://purl.uniprot.org/citations/21835666 | http://purl.uniprot.org/core/pages | "738-750"xsd:string |
| http://purl.uniprot.org/citations/21835666 | http://purl.uniprot.org/core/title | "The mechanism of tail-anchored protein insertion into the ER membrane."xsd:string |
| http://purl.uniprot.org/citations/21835666 | http://purl.uniprot.org/core/title | "The mechanism of tail-anchored protein insertion into the ER membrane."xsd:string |
| http://purl.uniprot.org/citations/21835666 | http://purl.uniprot.org/core/volume | "43"xsd:string |
| http://purl.uniprot.org/citations/21835666 | http://purl.uniprot.org/core/volume | "43"xsd:string |
| http://purl.uniprot.org/citations/21835666 | http://www.w3.org/2004/02/skos/core#exactMatch | http://purl.uniprot.org/pubmed/21835666 |
| http://purl.uniprot.org/citations/21835666 | http://www.w3.org/2004/02/skos/core#exactMatch | http://purl.uniprot.org/pubmed/21835666 |