| http://purl.uniprot.org/citations/21865551 | http://www.w3.org/1999/02/22-rdf-syntax-ns#type | http://purl.uniprot.org/core/Journal_Citation |
| http://purl.uniprot.org/citations/21865551 | http://www.w3.org/2000/01/rdf-schema#comment | "The P2X(7) receptor (P2X(7)R), an ATP-gated ion channel, has been implicated in the process of cell-to-cell fusion into multinucleated macrophages (MA), but its contribution to MA fusion driven by physiological/pathological stimuli is not clearly established. Based on several lines of evidence, we demonstrate that P2X(7)R is critical for the induction of multinucleated MA by the inflammatory cytokine GM-CSF: 1) pharmacological inhibition of P2X(7)R with oxidized ATP (oATP), KN-62, and the selective antagonist A740003 abrogated GM-CSF action on rat alveolar MA and murine peritoneal MA; 2) a murine J774 P2X(7) low MA clone, selected for defective P2X(7)R function, was unresponsive; 3) MA from mice lacking P2X(7)R failed to respond to GM-CSF, in contrast to wild-type. GM-CSF also stimulated ATP-induced membrane permeabilization in J774 P2X(7) high MA and rat alveolar MA, an effect absent in the P2X(7) low MA clone and inhibited by the P2X(7) blockers oATP and KN-62. Notably, the stimulatory effects of GM-CSF on pore formation and MA fusion were both inhibited by blocking functional Pannexin-1 (Panx-1), and GM-CSF failed to stimulate MA fusion in cells from Panx-1 knockout mice. We provide further evidence that extracellular ATP release from peritoneal MA is dependent on P2X(7) but not on Panx-1 expression and that its metabolism to adenosine mediates P2X(7)-dependent MA fusion. These data demonstrate that both P2X(7) and Panx-1 are required for GM-CSF promotion of MA fusion but likely act independently through different signaling pathway(s)."xsd:string |
| http://purl.uniprot.org/citations/21865551 | http://purl.org/dc/terms/identifier | "doi:10.4049/jimmunol.1002780"xsd:string |
| http://purl.uniprot.org/citations/21865551 | http://purl.uniprot.org/core/author | "Zhang B."xsd:string |
| http://purl.uniprot.org/citations/21865551 | http://purl.uniprot.org/core/author | "Lemaire I."xsd:string |
| http://purl.uniprot.org/citations/21865551 | http://purl.uniprot.org/core/author | "Di Virgilio F."xsd:string |
| http://purl.uniprot.org/citations/21865551 | http://purl.uniprot.org/core/author | "Falzoni S."xsd:string |
| http://purl.uniprot.org/citations/21865551 | http://purl.uniprot.org/core/author | "Pellegatti P."xsd:string |
| http://purl.uniprot.org/citations/21865551 | http://purl.uniprot.org/core/date | "2011"xsd:gYear |
| http://purl.uniprot.org/citations/21865551 | http://purl.uniprot.org/core/name | "J Immunol"xsd:string |
| http://purl.uniprot.org/citations/21865551 | http://purl.uniprot.org/core/pages | "3878-3887"xsd:string |
| http://purl.uniprot.org/citations/21865551 | http://purl.uniprot.org/core/title | "The P2X7 receptor and Pannexin-1 are both required for the promotion of multinucleated macrophages by the inflammatory cytokine GM-CSF."xsd:string |
| http://purl.uniprot.org/citations/21865551 | http://purl.uniprot.org/core/volume | "187"xsd:string |
| http://purl.uniprot.org/citations/21865551 | http://www.w3.org/2004/02/skos/core#exactMatch | http://purl.uniprot.org/pubmed/21865551 |
| http://purl.uniprot.org/citations/21865551 | http://xmlns.com/foaf/0.1/primaryTopicOf | https://pubmed.ncbi.nlm.nih.gov/21865551 |
| http://purl.uniprot.org/uniprot/#_A0A0G2KA39-mappedCitation-21865551 | http://www.w3.org/1999/02/22-rdf-syntax-ns#object | http://purl.uniprot.org/citations/21865551 |
| http://purl.uniprot.org/uniprot/#_E0X642-mappedCitation-21865551 | http://www.w3.org/1999/02/22-rdf-syntax-ns#object | http://purl.uniprot.org/citations/21865551 |
| http://purl.uniprot.org/uniprot/#_E0X643-mappedCitation-21865551 | http://www.w3.org/1999/02/22-rdf-syntax-ns#object | http://purl.uniprot.org/citations/21865551 |
| http://purl.uniprot.org/uniprot/#_A2VCP3-mappedCitation-21865551 | http://www.w3.org/1999/02/22-rdf-syntax-ns#object | http://purl.uniprot.org/citations/21865551 |
| http://purl.uniprot.org/uniprot/#_A6JNB1-mappedCitation-21865551 | http://www.w3.org/1999/02/22-rdf-syntax-ns#object | http://purl.uniprot.org/citations/21865551 |
| http://purl.uniprot.org/uniprot/#_B1PL19-mappedCitation-21865551 | http://www.w3.org/1999/02/22-rdf-syntax-ns#object | http://purl.uniprot.org/citations/21865551 |
| http://purl.uniprot.org/uniprot/#_C8YIX4-mappedCitation-21865551 | http://www.w3.org/1999/02/22-rdf-syntax-ns#object | http://purl.uniprot.org/citations/21865551 |
| http://purl.uniprot.org/uniprot/#_B6ETL5-mappedCitation-21865551 | http://www.w3.org/1999/02/22-rdf-syntax-ns#object | http://purl.uniprot.org/citations/21865551 |
| http://purl.uniprot.org/uniprot/#_E9PY07-mappedCitation-21865551 | http://www.w3.org/1999/02/22-rdf-syntax-ns#object | http://purl.uniprot.org/citations/21865551 |
| http://purl.uniprot.org/uniprot/#_F8WI93-mappedCitation-21865551 | http://www.w3.org/1999/02/22-rdf-syntax-ns#object | http://purl.uniprot.org/citations/21865551 |