| http://purl.uniprot.org/citations/21892598 | http://www.w3.org/1999/02/22-rdf-syntax-ns#type | http://purl.uniprot.org/core/Journal_Citation |
| http://purl.uniprot.org/citations/21892598 | http://www.w3.org/1999/02/22-rdf-syntax-ns#type | http://purl.uniprot.org/core/Journal_Citation |
| http://purl.uniprot.org/citations/21892598 | http://www.w3.org/2000/01/rdf-schema#comment | "Nitrilases from Aspergillus niger CBS 513.88, A. niger K10, Gibberella moniliformis, Neurospora crassa OR74A, and Penicillium marneffei ATCC 18224 were expressed in Escherichia coli BL21-Gold (DE3) after IPTG induction. N. crassa nitrilase exhibited the highest yield of 69,000 U L(-1) culture. Co-expression of chaperones (GroEL/ES in G. moniliformis and P. marneffei; GroEL/ES and trigger factor in N. crassa and A. niger CBS 513.88) enhanced the enzyme solubility. Specific activities of strains expressing the former two enzymes increased approximately fourfold upon co-expression of GroEL/ES. The enzyme from G. moniliformis (co-purified with GroEL) preferred benzonitrile as substrate (K(m) of 0.41 mM, V(max) of 9.7 μmol min(-1) mg(-1) protein). The P. marneffei enzyme (unstable in its purified state) exhibited the highest V(max) of 7.3 μmol min(-1) mg(-1) protein in cell-free extract, but also a high K(m) of 15.4 mM, for 4-cyanopyridine. The purified nitrilases from A. niger CBS 513.88 and N. crassa acted preferentially on phenylacetonitrile (K(m) of 3.4 and 2.0 mM, respectively; V(max) of 10.6 and 17.5 μmol min(-1) mg(-1) protein, respectively), and hydrolyzed also (R,S)-mandelonitrile with higher K(m) values. Significant amounts of amides were only formed by the G. moniliformis nitrilase from phenylacetonitrile and 4-cyanopyridine."xsd:string |
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| http://purl.uniprot.org/citations/21892598 | http://purl.org/dc/terms/identifier | "doi:10.1007/s00253-011-3525-7"xsd:string |
| http://purl.uniprot.org/citations/21892598 | http://purl.uniprot.org/core/author | "Kaplan O."xsd:string |
| http://purl.uniprot.org/citations/21892598 | http://purl.uniprot.org/core/author | "Kaplan O."xsd:string |
| http://purl.uniprot.org/citations/21892598 | http://purl.uniprot.org/core/author | "Martinkova L."xsd:string |
| http://purl.uniprot.org/citations/21892598 | http://purl.uniprot.org/core/author | "Martinkova L."xsd:string |
| http://purl.uniprot.org/citations/21892598 | http://purl.uniprot.org/core/author | "Vesela A.B."xsd:string |
| http://purl.uniprot.org/citations/21892598 | http://purl.uniprot.org/core/author | "Vesela A.B."xsd:string |
| http://purl.uniprot.org/citations/21892598 | http://purl.uniprot.org/core/author | "Bezouska K."xsd:string |
| http://purl.uniprot.org/citations/21892598 | http://purl.uniprot.org/core/author | "Bezouska K."xsd:string |
| http://purl.uniprot.org/citations/21892598 | http://purl.uniprot.org/core/author | "Felsberg J."xsd:string |
| http://purl.uniprot.org/citations/21892598 | http://purl.uniprot.org/core/author | "Felsberg J."xsd:string |
| http://purl.uniprot.org/citations/21892598 | http://purl.uniprot.org/core/author | "Kren V."xsd:string |
| http://purl.uniprot.org/citations/21892598 | http://purl.uniprot.org/core/author | "Kren V."xsd:string |
| http://purl.uniprot.org/citations/21892598 | http://purl.uniprot.org/core/author | "Kubac D."xsd:string |
| http://purl.uniprot.org/citations/21892598 | http://purl.uniprot.org/core/author | "Kubac D."xsd:string |
| http://purl.uniprot.org/citations/21892598 | http://purl.uniprot.org/core/author | "Malandra A."xsd:string |
| http://purl.uniprot.org/citations/21892598 | http://purl.uniprot.org/core/author | "Malandra A."xsd:string |
| http://purl.uniprot.org/citations/21892598 | http://purl.uniprot.org/core/author | "Petrickova A."xsd:string |
| http://purl.uniprot.org/citations/21892598 | http://purl.uniprot.org/core/author | "Petrickova A."xsd:string |
| http://purl.uniprot.org/citations/21892598 | http://purl.uniprot.org/core/author | "Rinagelova A."xsd:string |
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