| http://purl.uniprot.org/citations/21908715 | http://www.w3.org/1999/02/22-rdf-syntax-ns#type | http://purl.uniprot.org/core/Journal_Citation |
| http://purl.uniprot.org/citations/21908715 | http://www.w3.org/2000/01/rdf-schema#comment | "Cell migration plays a crucial role in numerous cellular functions, and alterations in the regulation of cell migration are required for invasive transformation of a tumor cell. While the mechanistic process of actin-based migration has been well documented, little is known as to the specific function of the nonmuscle actin isoforms in mammalian cells. Here, we present a comprehensive examination of γ-actin's role in cell migration using an RNAi approach. The partial suppression of γ-actin expression in SH-EP neuroblastoma cells resulted in a significant decrease in wound healing and transwell migration. Similarly, the knockdown of γ-actin significantly reduced speed of motility and severely affected the cell's ability to explore, which was, in part, due to a loss of cell polarity. Moreover, there was a significant increase in the size and number of paxillin-containing focal adhesions, coupled with a significant decrease in phosphorylated paxillin in γ-actin-knockdown cells. In addition, there was a significant increase in the phosphorylation of cofilin and myosin regulatory light chain, suggesting an overactivated Rho-associated kinase (ROCK) signaling pathway in γ-actin-knockdown cells. The alterations in the phosphorylation of paxillin and myosin regulatory light chain were unique to γ-actin and not β-actin knockdown. Inhibition of the ROCK pathway with the inhibitor Y-27632 restored the ability of γ-actin-knockdown cells to migrate. This study demonstrates γ-actin as a potential upstream regulator of ROCK mediated cell migration."xsd:string |
| http://purl.uniprot.org/citations/21908715 | http://purl.org/dc/terms/identifier | "doi:10.1096/fj.11-185447"xsd:string |
| http://purl.uniprot.org/citations/21908715 | http://purl.uniprot.org/core/author | "O'Neill G.M."xsd:string |
| http://purl.uniprot.org/citations/21908715 | http://purl.uniprot.org/core/author | "Chaponnier C."xsd:string |
| http://purl.uniprot.org/citations/21908715 | http://purl.uniprot.org/core/author | "Gunning P.W."xsd:string |
| http://purl.uniprot.org/citations/21908715 | http://purl.uniprot.org/core/author | "Kavallaris M."xsd:string |
| http://purl.uniprot.org/citations/21908715 | http://purl.uniprot.org/core/author | "Pasquier E."xsd:string |
| http://purl.uniprot.org/citations/21908715 | http://purl.uniprot.org/core/author | "Po'uha S.T."xsd:string |
| http://purl.uniprot.org/citations/21908715 | http://purl.uniprot.org/core/author | "Shum M.S."xsd:string |
| http://purl.uniprot.org/citations/21908715 | http://purl.uniprot.org/core/date | "2011"xsd:gYear |
| http://purl.uniprot.org/citations/21908715 | http://purl.uniprot.org/core/name | "FASEB J"xsd:string |
| http://purl.uniprot.org/citations/21908715 | http://purl.uniprot.org/core/pages | "4423-4433"xsd:string |
| http://purl.uniprot.org/citations/21908715 | http://purl.uniprot.org/core/title | "gamma-Actin regulates cell migration and modulates the ROCK signaling pathway."xsd:string |
| http://purl.uniprot.org/citations/21908715 | http://purl.uniprot.org/core/volume | "25"xsd:string |
| http://purl.uniprot.org/citations/21908715 | http://www.w3.org/2004/02/skos/core#exactMatch | http://purl.uniprot.org/pubmed/21908715 |
| http://purl.uniprot.org/citations/21908715 | http://xmlns.com/foaf/0.1/primaryTopicOf | https://pubmed.ncbi.nlm.nih.gov/21908715 |
| http://purl.uniprot.org/uniprot/#_B4E3A4-mappedCitation-21908715 | http://www.w3.org/1999/02/22-rdf-syntax-ns#object | http://purl.uniprot.org/citations/21908715 |
| http://purl.uniprot.org/uniprot/#_B4DVQ0-mappedCitation-21908715 | http://www.w3.org/1999/02/22-rdf-syntax-ns#object | http://purl.uniprot.org/citations/21908715 |
| http://purl.uniprot.org/uniprot/#_D9ZGF8-mappedCitation-21908715 | http://www.w3.org/1999/02/22-rdf-syntax-ns#object | http://purl.uniprot.org/citations/21908715 |
| http://purl.uniprot.org/uniprot/#_Q13464-mappedCitation-21908715 | http://www.w3.org/1999/02/22-rdf-syntax-ns#object | http://purl.uniprot.org/citations/21908715 |
| http://purl.uniprot.org/uniprot/#_P63261-mappedCitation-21908715 | http://www.w3.org/1999/02/22-rdf-syntax-ns#object | http://purl.uniprot.org/citations/21908715 |
| http://purl.uniprot.org/uniprot/#_Q562L5-mappedCitation-21908715 | http://www.w3.org/1999/02/22-rdf-syntax-ns#object | http://purl.uniprot.org/citations/21908715 |
| http://purl.uniprot.org/uniprot/#_Q562L6-mappedCitation-21908715 | http://www.w3.org/1999/02/22-rdf-syntax-ns#object | http://purl.uniprot.org/citations/21908715 |
| http://purl.uniprot.org/uniprot/#_Q562L9-mappedCitation-21908715 | http://www.w3.org/1999/02/22-rdf-syntax-ns#object | http://purl.uniprot.org/citations/21908715 |