| http://purl.uniprot.org/citations/21931855 | http://www.w3.org/1999/02/22-rdf-syntax-ns#type | http://purl.uniprot.org/core/Journal_Citation |
| http://purl.uniprot.org/citations/21931855 | http://www.w3.org/2000/01/rdf-schema#comment | "Reversible post-translational protein modifications such as SUMOylation add complexity to cardiac transcriptional regulation. The homeodomain transcription factor Nkx2-5/Csx is essential for heart specification and morphogenesis. It has been previously suggested that SUMOylation of lysine 51 (K51) of Nkx2-5 is essential for its DNA binding and transcriptional activation. Here, we confirm that SUMOylation strongly enhances Nkx2-5 transcriptional activity and that residue K51 of Nkx2-5 is a SUMOylation target. However, in a range of cultured cell lines we find that a point mutation of K51 to arginine (K51R) does not affect Nkx2-5 activity or DNA binding, suggesting the existence of additional Nkx2-5 SUMOylated residues. Using biochemical assays, we demonstrate that Nkx2-5 is SUMOylated on at least one additional site, and this is the predominant site in cardiac cells. The second site is either non-canonical or a "shifting" site, as mutation of predicted consensus sites and indeed every individual lysine in the context of the K51R mutation failed to impair Nkx2-5 transcriptional synergism with SUMO, or its nuclear localization and DNA binding. We also observe SUMOylation of Nkx2-5 cofactors, which may be critical to Nkx2-5 regulation. Our data reveal highly complex regulatory mechanisms driven by SUMOylation to modulate Nkx2-5 activity."xsd:string |
| http://purl.uniprot.org/citations/21931855 | http://purl.org/dc/terms/identifier | "doi:10.1371/journal.pone.0024812"xsd:string |
| http://purl.uniprot.org/citations/21931855 | http://purl.uniprot.org/core/author | "Dunwoodie S.L."xsd:string |
| http://purl.uniprot.org/citations/21931855 | http://purl.uniprot.org/core/author | "Lee S."xsd:string |
| http://purl.uniprot.org/citations/21931855 | http://purl.uniprot.org/core/author | "Sparrow D.B."xsd:string |
| http://purl.uniprot.org/citations/21931855 | http://purl.uniprot.org/core/author | "Harvey R.P."xsd:string |
| http://purl.uniprot.org/citations/21931855 | http://purl.uniprot.org/core/author | "Kurtenbach E."xsd:string |
| http://purl.uniprot.org/citations/21931855 | http://purl.uniprot.org/core/author | "Rosenthal N."xsd:string |
| http://purl.uniprot.org/citations/21931855 | http://purl.uniprot.org/core/author | "Mohun T."xsd:string |
| http://purl.uniprot.org/citations/21931855 | http://purl.uniprot.org/core/author | "Furtado M.B."xsd:string |
| http://purl.uniprot.org/citations/21931855 | http://purl.uniprot.org/core/author | "Xin L."xsd:string |
| http://purl.uniprot.org/citations/21931855 | http://purl.uniprot.org/core/author | "Costa M.W."xsd:string |
| http://purl.uniprot.org/citations/21931855 | http://purl.uniprot.org/core/author | "Martinez C.G."xsd:string |
| http://purl.uniprot.org/citations/21931855 | http://purl.uniprot.org/core/date | "2011"xsd:gYear |
| http://purl.uniprot.org/citations/21931855 | http://purl.uniprot.org/core/name | "PLoS One"xsd:string |
| http://purl.uniprot.org/citations/21931855 | http://purl.uniprot.org/core/pages | "e24812"xsd:string |
| http://purl.uniprot.org/citations/21931855 | http://purl.uniprot.org/core/title | "Complex SUMO-1 regulation of cardiac transcription factor Nkx2-5."xsd:string |
| http://purl.uniprot.org/citations/21931855 | http://purl.uniprot.org/core/volume | "6"xsd:string |
| http://purl.uniprot.org/citations/21931855 | http://www.w3.org/2004/02/skos/core#exactMatch | http://purl.uniprot.org/pubmed/21931855 |
| http://purl.uniprot.org/citations/21931855 | http://xmlns.com/foaf/0.1/primaryTopicOf | https://pubmed.ncbi.nlm.nih.gov/21931855 |
| http://purl.uniprot.org/uniprot/P61957#attribution-017938D8AF7D5CBA58B6EEEA78A570B8 | http://purl.uniprot.org/core/source | http://purl.uniprot.org/citations/21931855 |
| http://purl.uniprot.org/uniprot/P63166#attribution-9D6CADBE20280304C9BE81880BE6F00A | http://purl.uniprot.org/core/source | http://purl.uniprot.org/citations/21931855 |
| http://purl.uniprot.org/uniprot/#_D3Z794-mappedCitation-21931855 | http://www.w3.org/1999/02/22-rdf-syntax-ns#object | http://purl.uniprot.org/citations/21931855 |
| http://purl.uniprot.org/uniprot/#_A0A087WRQ4-mappedCitation-21931855 | http://www.w3.org/1999/02/22-rdf-syntax-ns#object | http://purl.uniprot.org/citations/21931855 |