| http://purl.uniprot.org/citations/21940635 | http://www.w3.org/1999/02/22-rdf-syntax-ns#type | http://purl.uniprot.org/core/Journal_Citation |
| http://purl.uniprot.org/citations/21940635 | http://www.w3.org/2000/01/rdf-schema#comment | "Rap1b is activated by platelet agonists and plays a critical role in integrin α(IIb)β(3) inside-out signaling and platelet aggregation. Here we show that agonist-induced Rap1b activation plays an important role in stimulating secretion of platelet granules. We also show that α(IIb)β(3) outside-in signaling can activate Rap1b, and integrin outside-in signaling-mediated Rap1b activation is important in facilitating platelet spreading on fibrinogen and clot retraction. Rap1b-deficient platelets had diminished ATP secretion and P-selectin expression induced by thrombin or collagen. Importantly, addition of low doses of ADP and/or fibrinogen restored aggregation of Rap1b-deficient platelets. Furthermore, we found that Rap1b was activated by platelet spreading on immobilized fibrinogen, a process that was not affected by P2Y(12) or TXA(2) receptor deficiency, but was inhibited by the selective Src inhibitor PP2, the PKC inhibitor Ro-31-8220, or the calcium chelator demethyl-1,2-bis(2-aminophenoxy)ethane-N,N,N',N'-tetraacetic acid tetrakis. Clot retraction was abolished, and platelet spreading on fibrinogen was diminished in Rap1b-deficient platelets compared with wild-type controls. The defects in clot retraction and spreading on fibrinogen of Rap1b-deficient platelets were not rescued by addition of MnCl(2), which elicits α(IIb)β(3) outside-in signaling in the absence of inside-out signaling. Thus, our results reveal two different activation mechanisms of Rap1b as well as novel functions of Rap1b in platelet secretion and in integrin α(IIb)β(3) outside-in signaling."xsd:string |
| http://purl.uniprot.org/citations/21940635 | http://purl.org/dc/terms/identifier | "doi:10.1074/jbc.m111.239608"xsd:string |
| http://purl.uniprot.org/citations/21940635 | http://purl.uniprot.org/core/author | "Li Z."xsd:string |
| http://purl.uniprot.org/citations/21940635 | http://purl.uniprot.org/core/author | "Zhang G."xsd:string |
| http://purl.uniprot.org/citations/21940635 | http://purl.uniprot.org/core/author | "Xiang B."xsd:string |
| http://purl.uniprot.org/citations/21940635 | http://purl.uniprot.org/core/author | "Ye S."xsd:string |
| http://purl.uniprot.org/citations/21940635 | http://purl.uniprot.org/core/author | "Morris A.J."xsd:string |
| http://purl.uniprot.org/citations/21940635 | http://purl.uniprot.org/core/author | "Gartner T.K."xsd:string |
| http://purl.uniprot.org/citations/21940635 | http://purl.uniprot.org/core/author | "Whiteheart S.W."xsd:string |
| http://purl.uniprot.org/citations/21940635 | http://purl.uniprot.org/core/author | "Smyth S.S."xsd:string |
| http://purl.uniprot.org/citations/21940635 | http://purl.uniprot.org/core/author | "White G.C."xsd:string |
| http://purl.uniprot.org/citations/21940635 | http://purl.uniprot.org/core/author | "Chrzanowska-Wodnicka M."xsd:string |
| http://purl.uniprot.org/citations/21940635 | http://purl.uniprot.org/core/date | "2011"xsd:gYear |
| http://purl.uniprot.org/citations/21940635 | http://purl.uniprot.org/core/name | "J Biol Chem"xsd:string |
| http://purl.uniprot.org/citations/21940635 | http://purl.uniprot.org/core/pages | "39466-39477"xsd:string |
| http://purl.uniprot.org/citations/21940635 | http://purl.uniprot.org/core/title | "Distinct roles for Rap1b protein in platelet secretion and integrin alphaIIbbeta3 outside-in signaling."xsd:string |
| http://purl.uniprot.org/citations/21940635 | http://purl.uniprot.org/core/volume | "286"xsd:string |
| http://purl.uniprot.org/citations/21940635 | http://www.w3.org/2004/02/skos/core#exactMatch | http://purl.uniprot.org/pubmed/21940635 |
| http://purl.uniprot.org/citations/21940635 | http://xmlns.com/foaf/0.1/primaryTopicOf | https://pubmed.ncbi.nlm.nih.gov/21940635 |
| http://purl.uniprot.org/uniprot/#_Q99JI6-mappedCitation-21940635 | http://www.w3.org/1999/02/22-rdf-syntax-ns#object | http://purl.uniprot.org/citations/21940635 |
| http://purl.uniprot.org/uniprot/#_Q52L50-mappedCitation-21940635 | http://www.w3.org/1999/02/22-rdf-syntax-ns#object | http://purl.uniprot.org/citations/21940635 |
| http://purl.uniprot.org/uniprot/Q99JI6 | http://purl.uniprot.org/core/mappedCitation | http://purl.uniprot.org/citations/21940635 |
| http://purl.uniprot.org/uniprot/Q52L50 | http://purl.uniprot.org/core/mappedCitation | http://purl.uniprot.org/citations/21940635 |