| http://purl.uniprot.org/citations/21944757 | http://www.w3.org/1999/02/22-rdf-syntax-ns#type | http://purl.uniprot.org/core/Journal_Citation |
| http://purl.uniprot.org/citations/21944757 | http://www.w3.org/2000/01/rdf-schema#comment | "Peptide nucleic acids (PNAs) bind duplex DNA in a sequence-specific manner, creating triplex structures that can provoke DNA repair and produce genome modification. CCR5 encodes a chemokine receptor required for HIV-1 entry into human cells, and individuals carrying mutations in this gene are resistant to HIV-1 infection. Transfection of human cells with PNAs targeted to the CCR5 gene, plus donor DNAs designed to introduce stop codons mimicking the naturally occurring CCR5-delta32 mutation, produced 2.46% targeted gene modification. CCR5 modification was confirmed at the DNA, RNA, and protein levels and was shown to confer resistance to infection with HIV-1. Targeting of CCR5 was achieved in human CD34(+) hematopoietic stem cells (HSCs) with subsequent engraftment into mice and persistence of the gene modification more than four months posttransplantation. This work suggests a therapeutic strategy for CCR5 knockout in HSCs from HIV-1-infected individuals, rendering cells resistant to HIV-1 and preserving immune system function."xsd:string |
| http://purl.uniprot.org/citations/21944757 | http://purl.org/dc/terms/identifier | "doi:10.1016/j.chembiol.2011.07.010"xsd:string |
| http://purl.uniprot.org/citations/21944757 | http://purl.uniprot.org/core/author | "Kumar P."xsd:string |
| http://purl.uniprot.org/citations/21944757 | http://purl.uniprot.org/core/author | "Shultz L.D."xsd:string |
| http://purl.uniprot.org/citations/21944757 | http://purl.uniprot.org/core/author | "Greiner D.L."xsd:string |
| http://purl.uniprot.org/citations/21944757 | http://purl.uniprot.org/core/author | "del Campo J."xsd:string |
| http://purl.uniprot.org/citations/21944757 | http://purl.uniprot.org/core/author | "Kutsch O."xsd:string |
| http://purl.uniprot.org/citations/21944757 | http://purl.uniprot.org/core/author | "Glazer P.M."xsd:string |
| http://purl.uniprot.org/citations/21944757 | http://purl.uniprot.org/core/author | "Leif J."xsd:string |
| http://purl.uniprot.org/citations/21944757 | http://purl.uniprot.org/core/author | "Bindra R."xsd:string |
| http://purl.uniprot.org/citations/21944757 | http://purl.uniprot.org/core/author | "Rogers F.A."xsd:string |
| http://purl.uniprot.org/citations/21944757 | http://purl.uniprot.org/core/author | "Schleifman E.B."xsd:string |
| http://purl.uniprot.org/citations/21944757 | http://purl.uniprot.org/core/author | "Uchil P."xsd:string |
| http://purl.uniprot.org/citations/21944757 | http://purl.uniprot.org/core/date | "2011"xsd:gYear |
| http://purl.uniprot.org/citations/21944757 | http://purl.uniprot.org/core/name | "Chem Biol"xsd:string |
| http://purl.uniprot.org/citations/21944757 | http://purl.uniprot.org/core/pages | "1189-1198"xsd:string |
| http://purl.uniprot.org/citations/21944757 | http://purl.uniprot.org/core/title | "Targeted disruption of the CCR5 gene in human hematopoietic stem cells stimulated by peptide nucleic acids."xsd:string |
| http://purl.uniprot.org/citations/21944757 | http://purl.uniprot.org/core/volume | "18"xsd:string |
| http://purl.uniprot.org/citations/21944757 | http://www.w3.org/2004/02/skos/core#exactMatch | http://purl.uniprot.org/pubmed/21944757 |
| http://purl.uniprot.org/citations/21944757 | http://xmlns.com/foaf/0.1/primaryTopicOf | https://pubmed.ncbi.nlm.nih.gov/21944757 |
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| http://purl.uniprot.org/uniprot/#_A0A089G6S9-mappedCitation-21944757 | http://www.w3.org/1999/02/22-rdf-syntax-ns#object | http://purl.uniprot.org/citations/21944757 |
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| http://purl.uniprot.org/uniprot/#_A0A089G7G0-mappedCitation-21944757 | http://www.w3.org/1999/02/22-rdf-syntax-ns#object | http://purl.uniprot.org/citations/21944757 |