| http://purl.uniprot.org/citations/21957239 | http://www.w3.org/1999/02/22-rdf-syntax-ns#type | http://purl.uniprot.org/core/Journal_Citation |
| http://purl.uniprot.org/citations/21957239 | http://www.w3.org/2000/01/rdf-schema#comment | "Dopaminergic signaling and plasticity are essential to numerous CNS functions and pathologies, including movement, cognition, and addiction. The amphetamine- and cocaine-sensitive dopamine (DA) transporter (DAT) tightly controls extracellular DA concentrations and half-life. DAT function and surface expression are not static but are dynamically modulated by membrane trafficking. We recently demonstrated that the DAT C terminus encodes a PKC-sensitive internalization signal that also suppresses basal DAT endocytosis. However, the cellular machinery governing regulated DAT trafficking is not well defined. In work presented here, we identified the Ras-like GTPase, Rin (for Ras-like in neurons) (Rit2), as a protein that interacts with the DAT C-terminal endocytic signal. Yeast two-hybrid, GST pull down and FRET studies establish that DAT and Rin directly interact, and colocalization studies reveal that DAT/Rin associations occur primarily in lipid raft microdomains. Coimmunoprecipitations demonstrate that PKC activation regulates Rin association with DAT. Perturbation of Rin function with GTPase mutants and shRNA-mediated Rin knockdown reveals that Rin is critical for PKC-mediated DAT internalization and functional downregulation. These results establish that Rin is a DAT-interacting protein that is required for PKC-regulated DAT trafficking. Moreover, this work suggests that Rin participates in regulated endocytosis."xsd:string |
| http://purl.uniprot.org/citations/21957239 | http://purl.org/dc/terms/identifier | "doi:10.1523/jneurosci.2649-11.2011"xsd:string |
| http://purl.uniprot.org/citations/21957239 | http://purl.uniprot.org/core/author | "Navaroli D.M."xsd:string |
| http://purl.uniprot.org/citations/21957239 | http://purl.uniprot.org/core/author | "Sitte H.H."xsd:string |
| http://purl.uniprot.org/citations/21957239 | http://purl.uniprot.org/core/author | "Lifshitz L.M."xsd:string |
| http://purl.uniprot.org/citations/21957239 | http://purl.uniprot.org/core/author | "King M.J."xsd:string |
| http://purl.uniprot.org/citations/21957239 | http://purl.uniprot.org/core/author | "Gabriel L."xsd:string |
| http://purl.uniprot.org/citations/21957239 | http://purl.uniprot.org/core/author | "Melikian H.E."xsd:string |
| http://purl.uniprot.org/citations/21957239 | http://purl.uniprot.org/core/author | "Uzelac Z."xsd:string |
| http://purl.uniprot.org/citations/21957239 | http://purl.uniprot.org/core/author | "Stevens Z.H."xsd:string |
| http://purl.uniprot.org/citations/21957239 | http://purl.uniprot.org/core/date | "2011"xsd:gYear |
| http://purl.uniprot.org/citations/21957239 | http://purl.uniprot.org/core/name | "J Neurosci"xsd:string |
| http://purl.uniprot.org/citations/21957239 | http://purl.uniprot.org/core/pages | "13758-13770"xsd:string |
| http://purl.uniprot.org/citations/21957239 | http://purl.uniprot.org/core/title | "The plasma membrane-associated GTPase Rin interacts with the dopamine transporter and is required for protein kinase C-regulated dopamine transporter trafficking."xsd:string |
| http://purl.uniprot.org/citations/21957239 | http://purl.uniprot.org/core/volume | "31"xsd:string |
| http://purl.uniprot.org/citations/21957239 | http://www.w3.org/2004/02/skos/core#exactMatch | http://purl.uniprot.org/pubmed/21957239 |
| http://purl.uniprot.org/citations/21957239 | http://xmlns.com/foaf/0.1/primaryTopicOf | https://pubmed.ncbi.nlm.nih.gov/21957239 |
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| http://purl.uniprot.org/uniprot/#_Q01959-mappedCitation-21957239 | http://www.w3.org/1999/02/22-rdf-syntax-ns#object | http://purl.uniprot.org/citations/21957239 |