| http://purl.uniprot.org/citations/21964294 | http://www.w3.org/1999/02/22-rdf-syntax-ns#type | http://purl.uniprot.org/core/Journal_Citation |
| http://purl.uniprot.org/citations/21964294 | http://www.w3.org/2000/01/rdf-schema#comment | "The coordination of cytoskeletal actin dynamics with gene expression reprogramming is emerging as a crucial mechanism to control diverse cellular processes, including cell migration, differentiation and neuronal circuit assembly. The actin-binding transcriptional coactivator MAL (also known as MRTF-A/MKL1/BSAC) senses G-actin concentration and transduces Rho GTPase signals to serum response factor (SRF). MAL rapidly shuttles between the cytoplasm and the nucleus in unstimulated cells but Rho-induced depletion of G-actin leads to MAL nuclear accumulation and activation of transcription of SRF:MAL-target genes. Although the molecular and structural basis of actin-regulated nucleocytoplasmic shuttling of MAL is not understood fully, it is proposed that nuclear import of MAL is mediated by importin α/β heterodimer, and that G-actin competes with importin α/β for the binding to MAL. Here we present structural, biochemical and cell biological evidence that MAL has a classical bipartite nuclear localization signal (NLS) in the N-terminal 'RPEL' domain containing Arg-Pro-X-X-X-Glu-Leu (RPEL) motifs. The NLS residues of MAL adopt an extended conformation and bind along the surface groove of importin-α, interacting with the major- and minor-NLS binding sites. We also present a crystal structure of wild-type MAL RPEL domain in complex with five G-actins. Comparison of the importin-α- and actin-complexes revealed that the binding of G-actins to MAL is associated with folding of NLS residues into a helical conformation that is inappropriate for importin-α recognition."xsd:string |
| http://purl.uniprot.org/citations/21964294 | http://purl.org/dc/terms/identifier | "doi:10.1016/j.bbrc.2011.09.079"xsd:string |
| http://purl.uniprot.org/citations/21964294 | http://purl.uniprot.org/core/author | "Hirano H."xsd:string |
| http://purl.uniprot.org/citations/21964294 | http://purl.uniprot.org/core/author | "Matsuura Y."xsd:string |
| http://purl.uniprot.org/citations/21964294 | http://purl.uniprot.org/core/date | "2011"xsd:gYear |
| http://purl.uniprot.org/citations/21964294 | http://purl.uniprot.org/core/name | "Biochem Biophys Res Commun"xsd:string |
| http://purl.uniprot.org/citations/21964294 | http://purl.uniprot.org/core/pages | "373-378"xsd:string |
| http://purl.uniprot.org/citations/21964294 | http://purl.uniprot.org/core/title | "Sensing actin dynamics: structural basis for G-actin-sensitive nuclear import of MAL."xsd:string |
| http://purl.uniprot.org/citations/21964294 | http://purl.uniprot.org/core/volume | "414"xsd:string |
| http://purl.uniprot.org/citations/21964294 | http://www.w3.org/2004/02/skos/core#exactMatch | http://purl.uniprot.org/pubmed/21964294 |
| http://purl.uniprot.org/citations/21964294 | http://xmlns.com/foaf/0.1/primaryTopicOf | https://pubmed.ncbi.nlm.nih.gov/21964294 |
| http://purl.uniprot.org/uniprot/#_P52293-mappedCitation-21964294 | http://www.w3.org/1999/02/22-rdf-syntax-ns#object | http://purl.uniprot.org/citations/21964294 |
| http://purl.uniprot.org/uniprot/#_P68135-mappedCitation-21964294 | http://www.w3.org/1999/02/22-rdf-syntax-ns#object | http://purl.uniprot.org/citations/21964294 |
| http://purl.uniprot.org/uniprot/#_Q3U1I6-mappedCitation-21964294 | http://www.w3.org/1999/02/22-rdf-syntax-ns#object | http://purl.uniprot.org/citations/21964294 |
| http://purl.uniprot.org/uniprot/#_Q3U3V7-mappedCitation-21964294 | http://www.w3.org/1999/02/22-rdf-syntax-ns#object | http://purl.uniprot.org/citations/21964294 |
| http://purl.uniprot.org/uniprot/#_Q8K4J6-mappedCitation-21964294 | http://www.w3.org/1999/02/22-rdf-syntax-ns#object | http://purl.uniprot.org/citations/21964294 |
| http://purl.uniprot.org/uniprot/Q8K4J6 | http://purl.uniprot.org/core/mappedCitation | http://purl.uniprot.org/citations/21964294 |
| http://purl.uniprot.org/uniprot/Q3U3V7 | http://purl.uniprot.org/core/mappedCitation | http://purl.uniprot.org/citations/21964294 |
| http://purl.uniprot.org/uniprot/Q3U1I6 | http://purl.uniprot.org/core/mappedCitation | http://purl.uniprot.org/citations/21964294 |
| http://purl.uniprot.org/uniprot/P68135 | http://purl.uniprot.org/core/mappedCitation | http://purl.uniprot.org/citations/21964294 |
| http://purl.uniprot.org/uniprot/P52293 | http://purl.uniprot.org/core/mappedCitation | http://purl.uniprot.org/citations/21964294 |