| http://purl.uniprot.org/citations/21965542 | http://www.w3.org/1999/02/22-rdf-syntax-ns#type | http://purl.uniprot.org/core/Journal_Citation |
| http://purl.uniprot.org/citations/21965542 | http://www.w3.org/1999/02/22-rdf-syntax-ns#type | http://purl.uniprot.org/core/Journal_Citation |
| http://purl.uniprot.org/citations/21965542 | http://www.w3.org/2000/01/rdf-schema#comment | "Metazoan spliced leader (SL) trans-splicing generates mRNAs with an m(2,2,7)G-cap and a common downstream SL RNA sequence. The mechanism for eIF4E binding an m²²⁷G-cap is unknown. Here, we describe the first structure of an eIF4E with an m(2,2,7)G-cap and compare it to the cognate m⁷G-eIF4E complex. These structures and Nuclear Magnetic Resonance (NMR) data indicate that the nematode Ascaris suum eIF4E binds the two different caps in a similar manner except for the loss of a single hydrogen bond on binding the m(2,2,7)G-cap. Nematode and mammalian eIF4E both have a low affinity for m(2,2,7)G-cap compared with the m⁷G-cap. Nematode eIF4E binding to the m⁷G-cap, m(2,2,7)G-cap and the m(2,2,7)G-SL 22-nt RNA leads to distinct eIF4E conformational changes. Additional interactions occur between Ascaris eIF4E and the SL on binding the m(2,2,7)G-SL. We propose interactions between Ascaris eIF4E and the SL impact eIF4G and contribute to translation initiation, whereas these interactions do not occur when only the m(2,2,7)G-cap is present. These data have implications for the contribution of 5'-UTRs in mRNA translation and the function of different eIF4E isoforms."xsd:string |
| http://purl.uniprot.org/citations/21965542 | http://purl.org/dc/terms/identifier | "doi:10.1093/nar/gkr650"xsd:string |
| http://purl.uniprot.org/citations/21965542 | http://purl.org/dc/terms/identifier | "doi:10.1093/nar/gkr650"xsd:string |
| http://purl.uniprot.org/citations/21965542 | http://purl.uniprot.org/core/author | "Liu W."xsd:string |
| http://purl.uniprot.org/citations/21965542 | http://purl.uniprot.org/core/author | "Liu W."xsd:string |
| http://purl.uniprot.org/citations/21965542 | http://purl.uniprot.org/core/author | "Zhao R."xsd:string |
| http://purl.uniprot.org/citations/21965542 | http://purl.uniprot.org/core/author | "Zhao R."xsd:string |
| http://purl.uniprot.org/citations/21965542 | http://purl.uniprot.org/core/author | "Darzynkiewicz E."xsd:string |
| http://purl.uniprot.org/citations/21965542 | http://purl.uniprot.org/core/author | "Darzynkiewicz E."xsd:string |
| http://purl.uniprot.org/citations/21965542 | http://purl.uniprot.org/core/author | "Stepinski J."xsd:string |
| http://purl.uniprot.org/citations/21965542 | http://purl.uniprot.org/core/author | "Stepinski J."xsd:string |
| http://purl.uniprot.org/citations/21965542 | http://purl.uniprot.org/core/author | "Davis R.E."xsd:string |
| http://purl.uniprot.org/citations/21965542 | http://purl.uniprot.org/core/author | "Davis R.E."xsd:string |
| http://purl.uniprot.org/citations/21965542 | http://purl.uniprot.org/core/author | "Jones D.N."xsd:string |
| http://purl.uniprot.org/citations/21965542 | http://purl.uniprot.org/core/author | "Jones D.N."xsd:string |
| http://purl.uniprot.org/citations/21965542 | http://purl.uniprot.org/core/author | "Wallace A."xsd:string |
| http://purl.uniprot.org/citations/21965542 | http://purl.uniprot.org/core/author | "Wallace A."xsd:string |
| http://purl.uniprot.org/citations/21965542 | http://purl.uniprot.org/core/author | "Jankowska-Anyszka M."xsd:string |
| http://purl.uniprot.org/citations/21965542 | http://purl.uniprot.org/core/author | "Jankowska-Anyszka M."xsd:string |
| http://purl.uniprot.org/citations/21965542 | http://purl.uniprot.org/core/author | "Stolarski R."xsd:string |
| http://purl.uniprot.org/citations/21965542 | http://purl.uniprot.org/core/author | "Stolarski R."xsd:string |
| http://purl.uniprot.org/citations/21965542 | http://purl.uniprot.org/core/author | "Dickson L."xsd:string |
| http://purl.uniprot.org/citations/21965542 | http://purl.uniprot.org/core/author | "Dickson L."xsd:string |