| http://purl.uniprot.org/citations/21974932 | http://www.w3.org/1999/02/22-rdf-syntax-ns#type | http://purl.uniprot.org/core/Journal_Citation |
| http://purl.uniprot.org/citations/21974932 | http://www.w3.org/2000/01/rdf-schema#comment | "Type 2 diabetes is a heterogeneous disorder caused by concomitant impairment of insulin secretion by pancreatic β cells and of insulin action in peripheral target tissues. Studies with inhibitors and agonists established a role for PLC in the regulation of insulin secretion but did not distinguish between effects due to nuclear or cytoplasmic PLC signaling pathways that act in a distinct fashion. We report that in MIN6 β cells, PLCβ1 localized in both nucleus and cytoplasm, PLCδ4 in the nucleus, and PLCγ1 in the cytoplasm. By silencing each isoform, we observed that they all affected glucose-induced insulin release both at basal and high glucose concentrations. To elucidate the molecular basis of PLC regulation, we focused on peroxisome proliferator-activated receptor-γ (PPARγ), a nuclear receptor transcription factor that regulates genes critical to β-cell maintenance and functions. Silencing of PLCβ1 and PLCδ4 resulted in a decrease in the PPARγ mRNA level. By means of a PPARγ-promoter-luciferase assay, the decrease could be attributed to a PLC action on the PPARγ-promoter region. The effect was specifically observed on silencing of the nuclear and not the cytoplasmic PLC. These findings highlight a novel pathway by which nuclear PLCs affect insulin secretion and identify PPARγ as a novel molecular target of nuclear PLCs."xsd:string |
| http://purl.uniprot.org/citations/21974932 | http://purl.org/dc/terms/identifier | "doi:10.1096/fj.11-186510"xsd:string |
| http://purl.uniprot.org/citations/21974932 | http://purl.uniprot.org/core/author | "Bavelloni A."xsd:string |
| http://purl.uniprot.org/citations/21974932 | http://purl.uniprot.org/core/author | "Cocco L."xsd:string |
| http://purl.uniprot.org/citations/21974932 | http://purl.uniprot.org/core/author | "Billi A.M."xsd:string |
| http://purl.uniprot.org/citations/21974932 | http://purl.uniprot.org/core/author | "Faenza I."xsd:string |
| http://purl.uniprot.org/citations/21974932 | http://purl.uniprot.org/core/author | "Piazzi M."xsd:string |
| http://purl.uniprot.org/citations/21974932 | http://purl.uniprot.org/core/author | "Fiume R."xsd:string |
| http://purl.uniprot.org/citations/21974932 | http://purl.uniprot.org/core/author | "Ramazzotti G."xsd:string |
| http://purl.uniprot.org/citations/21974932 | http://purl.uniprot.org/core/date | "2012"xsd:gYear |
| http://purl.uniprot.org/citations/21974932 | http://purl.uniprot.org/core/name | "FASEB J"xsd:string |
| http://purl.uniprot.org/citations/21974932 | http://purl.uniprot.org/core/pages | "203-210"xsd:string |
| http://purl.uniprot.org/citations/21974932 | http://purl.uniprot.org/core/title | "Nuclear PLCs affect insulin secretion by targeting PPARgamma in pancreatic beta cells."xsd:string |
| http://purl.uniprot.org/citations/21974932 | http://purl.uniprot.org/core/volume | "26"xsd:string |
| http://purl.uniprot.org/citations/21974932 | http://www.w3.org/2004/02/skos/core#exactMatch | http://purl.uniprot.org/pubmed/21974932 |
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