| http://purl.uniprot.org/citations/21990363 | http://www.w3.org/1999/02/22-rdf-syntax-ns#type | http://purl.uniprot.org/core/Journal_Citation |
| http://purl.uniprot.org/citations/21990363 | http://www.w3.org/1999/02/22-rdf-syntax-ns#type | http://purl.uniprot.org/core/Journal_Citation |
| http://purl.uniprot.org/citations/21990363 | http://www.w3.org/2000/01/rdf-schema#comment | "The enzyme carnitine palmitoyltransferase 1 (CPT1), which is anchored in the outer mitochondrial membrane (OMM), controls the rate-limiting step in fatty acid β-oxidation in mammalian tissues. It is inhibited by malonyl-CoA, the first intermediate of fatty acid synthesis, and it responds to OMM curvature and lipid characteristics, which reflect long term nutrient/hormone availability. Here, we show that the N-terminal regulatory domain (N) of CPT1A can adopt two complex amphiphilic structural states, termed Nα and Nβ, that interchange in a switch-like manner in response to offered binding surface curvature. Structure-based site-directed mutageneses of native CPT1A suggest Nα to be inhibitory and Nβ to be noninhibitory, with the relative Nα/Nβ ratio setting the prevalent malonyl-CoA sensitivity of the enzyme. Based on the amphiphilic nature of N and molecular modeling, we propose malonyl-CoA sensitivity to be coupled to the properties of the OMM by Nα-OMM associations that alter the Nα/Nβ ratio. For enzymes residing at the membrane-water interface, this constitutes an integrative regulatory mechanism of exceptional sophistication."xsd:string |
| http://purl.uniprot.org/citations/21990363 | http://purl.org/dc/terms/identifier | "doi:10.1074/jbc.m111.306951"xsd:string |
| http://purl.uniprot.org/citations/21990363 | http://purl.org/dc/terms/identifier | "doi:10.1074/jbc.m111.306951"xsd:string |
| http://purl.uniprot.org/citations/21990363 | http://purl.uniprot.org/core/author | "Ulmer T.S."xsd:string |
| http://purl.uniprot.org/citations/21990363 | http://purl.uniprot.org/core/author | "Ulmer T.S."xsd:string |
| http://purl.uniprot.org/citations/21990363 | http://purl.uniprot.org/core/author | "Estolt-Povedano S."xsd:string |
| http://purl.uniprot.org/citations/21990363 | http://purl.uniprot.org/core/author | "Estolt-Povedano S."xsd:string |
| http://purl.uniprot.org/citations/21990363 | http://purl.uniprot.org/core/author | "Rao J.N."xsd:string |
| http://purl.uniprot.org/citations/21990363 | http://purl.uniprot.org/core/author | "Rao J.N."xsd:string |
| http://purl.uniprot.org/citations/21990363 | http://purl.uniprot.org/core/author | "Warren G.Z."xsd:string |
| http://purl.uniprot.org/citations/21990363 | http://purl.uniprot.org/core/author | "Warren G.Z."xsd:string |
| http://purl.uniprot.org/citations/21990363 | http://purl.uniprot.org/core/author | "Zammit V.A."xsd:string |
| http://purl.uniprot.org/citations/21990363 | http://purl.uniprot.org/core/author | "Zammit V.A."xsd:string |
| http://purl.uniprot.org/citations/21990363 | http://purl.uniprot.org/core/date | "2011"xsd:gYear |
| http://purl.uniprot.org/citations/21990363 | http://purl.uniprot.org/core/date | "2011"xsd:gYear |
| http://purl.uniprot.org/citations/21990363 | http://purl.uniprot.org/core/name | "J. Biol. Chem."xsd:string |
| http://purl.uniprot.org/citations/21990363 | http://purl.uniprot.org/core/name | "J. Biol. Chem."xsd:string |
| http://purl.uniprot.org/citations/21990363 | http://purl.uniprot.org/core/pages | "42545-42554"xsd:string |
| http://purl.uniprot.org/citations/21990363 | http://purl.uniprot.org/core/pages | "42545-42554"xsd:string |
| http://purl.uniprot.org/citations/21990363 | http://purl.uniprot.org/core/title | "An environment-dependent structural switch underlies the regulation of carnitine palmitoyltransferase 1A."xsd:string |
| http://purl.uniprot.org/citations/21990363 | http://purl.uniprot.org/core/title | "An environment-dependent structural switch underlies the regulation of carnitine palmitoyltransferase 1A."xsd:string |
| http://purl.uniprot.org/citations/21990363 | http://purl.uniprot.org/core/volume | "286"xsd:string |
| http://purl.uniprot.org/citations/21990363 | http://purl.uniprot.org/core/volume | "286"xsd:string |